Analysis of lipoprotein apoproteins by SDS-gel filtration column chromatography

Analysis of lipoprotein apoproteins by SDS-gel filtration column chromatography

Rat plasma, containing 125I-labeled triglyceride-rich lipoprotein, was mixed following lipid extraction with 10% SDS buffer and analyzed by gel filtration chromatography on columns using an elution buffer containing 1% SDS. Labeled apoproteins were separated into apo B, apo E, and apo C radioactivit...

Full description

Bibliographic Details
Main Authors: C E Sparks, J B Marsh
Format: Article
Language:English
Published: Elsevier 1981-03-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S002222752034966X
id doaj-f0732882b1fd441c9fdcbff4e0ba5079
record_format Article
spelling doaj-f0732882b1fd441c9fdcbff4e0ba50792021-04-24T05:48:41ZengElsevierJournal of Lipid Research0022-22751981-03-01223514518Analysis of lipoprotein apoproteins by SDS-gel filtration column chromatographyC E SparksJ B MarshRat plasma, containing 125I-labeled triglyceride-rich lipoprotein, was mixed following lipid extraction with 10% SDS buffer and analyzed by gel filtration chromatography on columns using an elution buffer containing 1% SDS. Labeled apoproteins were separated into apo B, apo E, and apo C radioactivity peaks. Labeled peptides, tyrosine, and iodide were also resolved by this method. Isolated lipoprotein fractions were separated into the same components. The method offers the advantages of quantitative radioactivity recovery, large sample volume, and resolution of two apo B proteins.http://www.sciencedirect.com/science/article/pii/S002222752034966X
collection DOAJ
language English
format Article
sources DOAJ
author C E Sparks
J B Marsh
spellingShingle C E Sparks
J B Marsh
Analysis of lipoprotein apoproteins by SDS-gel filtration column chromatography
Journal of Lipid Research
author_facet C E Sparks
J B Marsh
author_sort C E Sparks
title Analysis of lipoprotein apoproteins by SDS-gel filtration column chromatography
title_short Analysis of lipoprotein apoproteins by SDS-gel filtration column chromatography
title_full Analysis of lipoprotein apoproteins by SDS-gel filtration column chromatography
title_fullStr Analysis of lipoprotein apoproteins by SDS-gel filtration column chromatography
title_full_unstemmed Analysis of lipoprotein apoproteins by SDS-gel filtration column chromatography
title_sort analysis of lipoprotein apoproteins by sds-gel filtration column chromatography
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1981-03-01
description Rat plasma, containing 125I-labeled triglyceride-rich lipoprotein, was mixed following lipid extraction with 10% SDS buffer and analyzed by gel filtration chromatography on columns using an elution buffer containing 1% SDS. Labeled apoproteins were separated into apo B, apo E, and apo C radioactivity peaks. Labeled peptides, tyrosine, and iodide were also resolved by this method. Isolated lipoprotein fractions were separated into the same components. The method offers the advantages of quantitative radioactivity recovery, large sample volume, and resolution of two apo B proteins.
url http://www.sciencedirect.com/science/article/pii/S002222752034966X
work_keys_str_mv AT cesparks analysisoflipoproteinapoproteinsbysdsgelfiltrationcolumnchromatography
AT jbmarsh analysisoflipoproteinapoproteinsbysdsgelfiltrationcolumnchromatography
_version_ 1721511993048825856

Similar Items