Analysis of lipoprotein apoproteins by SDS-gel filtration column chromatography
Rat plasma, containing 125I-labeled triglyceride-rich lipoprotein, was mixed following lipid extraction with 10% SDS buffer and analyzed by gel filtration chromatography on columns using an elution buffer containing 1% SDS. Labeled apoproteins were separated into apo B, apo E, and apo C radioactivit...
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1981-03-01
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Series: | Journal of Lipid Research |
Online Access: | http://www.sciencedirect.com/science/article/pii/S002222752034966X |
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doaj-f0732882b1fd441c9fdcbff4e0ba50792021-04-24T05:48:41ZengElsevierJournal of Lipid Research0022-22751981-03-01223514518Analysis of lipoprotein apoproteins by SDS-gel filtration column chromatographyC E SparksJ B MarshRat plasma, containing 125I-labeled triglyceride-rich lipoprotein, was mixed following lipid extraction with 10% SDS buffer and analyzed by gel filtration chromatography on columns using an elution buffer containing 1% SDS. Labeled apoproteins were separated into apo B, apo E, and apo C radioactivity peaks. Labeled peptides, tyrosine, and iodide were also resolved by this method. Isolated lipoprotein fractions were separated into the same components. The method offers the advantages of quantitative radioactivity recovery, large sample volume, and resolution of two apo B proteins.http://www.sciencedirect.com/science/article/pii/S002222752034966X |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
C E Sparks J B Marsh |
spellingShingle |
C E Sparks J B Marsh Analysis of lipoprotein apoproteins by SDS-gel filtration column chromatography Journal of Lipid Research |
author_facet |
C E Sparks J B Marsh |
author_sort |
C E Sparks |
title |
Analysis of lipoprotein apoproteins by SDS-gel filtration column chromatography |
title_short |
Analysis of lipoprotein apoproteins by SDS-gel filtration column chromatography |
title_full |
Analysis of lipoprotein apoproteins by SDS-gel filtration column chromatography |
title_fullStr |
Analysis of lipoprotein apoproteins by SDS-gel filtration column chromatography |
title_full_unstemmed |
Analysis of lipoprotein apoproteins by SDS-gel filtration column chromatography |
title_sort |
analysis of lipoprotein apoproteins by sds-gel filtration column chromatography |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
1981-03-01 |
description |
Rat plasma, containing 125I-labeled triglyceride-rich lipoprotein, was mixed following lipid extraction with 10% SDS buffer and analyzed by gel filtration chromatography on columns using an elution buffer containing 1% SDS. Labeled apoproteins were separated into apo B, apo E, and apo C radioactivity peaks. Labeled peptides, tyrosine, and iodide were also resolved by this method. Isolated lipoprotein fractions were separated into the same components. The method offers the advantages of quantitative radioactivity recovery, large sample volume, and resolution of two apo B proteins. |
url |
http://www.sciencedirect.com/science/article/pii/S002222752034966X |
work_keys_str_mv |
AT cesparks analysisoflipoproteinapoproteinsbysdsgelfiltrationcolumnchromatography AT jbmarsh analysisoflipoproteinapoproteinsbysdsgelfiltrationcolumnchromatography |
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1721511993048825856 |