Ginsenoside Rg1 augments oxidative metabolism and anabolic response of skeletal muscle in mice
Background: The ginsenoside Rg1 has been shown to exert various pharmacological activities with health benefits. Previously, we have reported that Rg1 promoted myogenic differentiation and myotube growth in C2C12 myoblasts. In this study, the in vivo effect of Rg1 on fiber-type composition and oxida...
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doaj-f08cc5f30efb4f8ab0a78d960a10fdbd2020-11-24T21:48:40ZengElsevierJournal of Ginseng Research1226-84532019-07-01433475481Ginsenoside Rg1 augments oxidative metabolism and anabolic response of skeletal muscle in miceHyeon-Ju Jeong0Hyun-Kyung So1Ayoung Jo2Hye-Been Kim3Sang-Jin Lee4Gyu-Un Bae5Jong-Sun Kang6Department of Molecular Cell Biology, Samsung Medical Center, Single Cell Network Research Center, Sungkyunkwan University School of Medicine, Suwon, Republic of KoreaDepartment of Molecular Cell Biology, Samsung Medical Center, Single Cell Network Research Center, Sungkyunkwan University School of Medicine, Suwon, Republic of KoreaResearch Center for Cell Fate Control, Research Institute of Pharmaceutical Science, College of Pharmacy, Sookmyung Women's University, Seoul, KoreaDepartment of Molecular Cell Biology, Samsung Medical Center, Single Cell Network Research Center, Sungkyunkwan University School of Medicine, Suwon, Republic of KoreaResearch Center for Cell Fate Control, Research Institute of Pharmaceutical Science, College of Pharmacy, Sookmyung Women's University, Seoul, KoreaResearch Center for Cell Fate Control, Research Institute of Pharmaceutical Science, College of Pharmacy, Sookmyung Women's University, Seoul, Korea; Corresponding author. Research Center for Cell Fate Control, Research Institute of Pharmaceutical Science, College of Pharmacy, Sookmyung Women's University, Cheongpa-ro 47-gil 100, Yongsan-Gu, Seoul 04310, Republic of Korea.Department of Molecular Cell Biology, Samsung Medical Center, Single Cell Network Research Center, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea; Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, Republic of Korea; Corresponding author. Department of Molecular Cell Biology, Samsung Medical Center, Single Cell Network Research Center, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.Background: The ginsenoside Rg1 has been shown to exert various pharmacological activities with health benefits. Previously, we have reported that Rg1 promoted myogenic differentiation and myotube growth in C2C12 myoblasts. In this study, the in vivo effect of Rg1 on fiber-type composition and oxidative metabolism in skeletal muscle was examined. Methods: To examine the effect of Rg1 on skeletal muscle, 3-month-old mice were treated with Rg1 for 5 weeks. To assess muscle strength, grip strength tests were performed, and the lower hind limb muscles were harvested, followed by various detailed analysis, such as histological staining, immunoblotting, immunostaining, and real-time quantitative reverse transcription polymerase chain reaction. In addition, to verify the in vivo data, primary myoblasts isolated from mice were treated with Rg1, and the Rg1 effect on myotube growth was examined by immunoblotting and immunostaining analysis. Results: Rg1 treatment increased the expression of myosin heavy chain isoforms characteristic for both oxidative and glycolytic muscle fibers; increased myofiber sizes were accompanied by enhanced muscle strength. Rg1 treatment also enhanced oxidative muscle metabolism with elevated oxidative phosphorylation proteins. Furthermore, Rg1-treated muscles exhibited increased levels of anabolic S6 kinase signaling. Conclusion: Rg1 improves muscle functionality via enhancing muscle gene expression and oxidative muscle metabolism in mice. Keywords: Atrophy, Exercise-specific signaling, Ginsenoside, Rg1, Skeletal musclehttp://www.sciencedirect.com/science/article/pii/S1226845318300083 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hyeon-Ju Jeong Hyun-Kyung So Ayoung Jo Hye-Been Kim Sang-Jin Lee Gyu-Un Bae Jong-Sun Kang |
spellingShingle |
Hyeon-Ju Jeong Hyun-Kyung So Ayoung Jo Hye-Been Kim Sang-Jin Lee Gyu-Un Bae Jong-Sun Kang Ginsenoside Rg1 augments oxidative metabolism and anabolic response of skeletal muscle in mice Journal of Ginseng Research |
author_facet |
Hyeon-Ju Jeong Hyun-Kyung So Ayoung Jo Hye-Been Kim Sang-Jin Lee Gyu-Un Bae Jong-Sun Kang |
author_sort |
Hyeon-Ju Jeong |
title |
Ginsenoside Rg1 augments oxidative metabolism and anabolic response of skeletal muscle in mice |
title_short |
Ginsenoside Rg1 augments oxidative metabolism and anabolic response of skeletal muscle in mice |
title_full |
Ginsenoside Rg1 augments oxidative metabolism and anabolic response of skeletal muscle in mice |
title_fullStr |
Ginsenoside Rg1 augments oxidative metabolism and anabolic response of skeletal muscle in mice |
title_full_unstemmed |
Ginsenoside Rg1 augments oxidative metabolism and anabolic response of skeletal muscle in mice |
title_sort |
ginsenoside rg1 augments oxidative metabolism and anabolic response of skeletal muscle in mice |
publisher |
Elsevier |
series |
Journal of Ginseng Research |
issn |
1226-8453 |
publishDate |
2019-07-01 |
description |
Background: The ginsenoside Rg1 has been shown to exert various pharmacological activities with health benefits. Previously, we have reported that Rg1 promoted myogenic differentiation and myotube growth in C2C12 myoblasts. In this study, the in vivo effect of Rg1 on fiber-type composition and oxidative metabolism in skeletal muscle was examined. Methods: To examine the effect of Rg1 on skeletal muscle, 3-month-old mice were treated with Rg1 for 5 weeks. To assess muscle strength, grip strength tests were performed, and the lower hind limb muscles were harvested, followed by various detailed analysis, such as histological staining, immunoblotting, immunostaining, and real-time quantitative reverse transcription polymerase chain reaction. In addition, to verify the in vivo data, primary myoblasts isolated from mice were treated with Rg1, and the Rg1 effect on myotube growth was examined by immunoblotting and immunostaining analysis. Results: Rg1 treatment increased the expression of myosin heavy chain isoforms characteristic for both oxidative and glycolytic muscle fibers; increased myofiber sizes were accompanied by enhanced muscle strength. Rg1 treatment also enhanced oxidative muscle metabolism with elevated oxidative phosphorylation proteins. Furthermore, Rg1-treated muscles exhibited increased levels of anabolic S6 kinase signaling. Conclusion: Rg1 improves muscle functionality via enhancing muscle gene expression and oxidative muscle metabolism in mice. Keywords: Atrophy, Exercise-specific signaling, Ginsenoside, Rg1, Skeletal muscle |
url |
http://www.sciencedirect.com/science/article/pii/S1226845318300083 |
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