Characteristics of microbial communities and intestinal pathogenic bacteria for migrated Larus ridibundus in southwest China
Abstract Larus ridibundus, a migratory wild bird, has become one of the most popular gull species in southwest China in recent years. There has been no information on the gut microbiota and intestinal pathogenic bacteria configuration in wild L. ridibundus, even though the public are in close contac...
Main Authors: | , , , , , , , , , |
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Format: | Article |
Language: | English |
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Wiley
2019-04-01
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Series: | MicrobiologyOpen |
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Online Access: | https://doi.org/10.1002/mbo3.693 |
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doaj-f0942c51c62f46e38780ac55c53a87f3 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Feng Liao Wenpeng Gu Duo Li Junrong Liang Xiaoqing Fu Wen Xu Ran Duan Xin Wang Huaiqi Jing Jiejie Dai |
spellingShingle |
Feng Liao Wenpeng Gu Duo Li Junrong Liang Xiaoqing Fu Wen Xu Ran Duan Xin Wang Huaiqi Jing Jiejie Dai Characteristics of microbial communities and intestinal pathogenic bacteria for migrated Larus ridibundus in southwest China MicrobiologyOpen gut microbiota intestinal pathogenic bacteria Larus ridibundus southwest China |
author_facet |
Feng Liao Wenpeng Gu Duo Li Junrong Liang Xiaoqing Fu Wen Xu Ran Duan Xin Wang Huaiqi Jing Jiejie Dai |
author_sort |
Feng Liao |
title |
Characteristics of microbial communities and intestinal pathogenic bacteria for migrated Larus ridibundus in southwest China |
title_short |
Characteristics of microbial communities and intestinal pathogenic bacteria for migrated Larus ridibundus in southwest China |
title_full |
Characteristics of microbial communities and intestinal pathogenic bacteria for migrated Larus ridibundus in southwest China |
title_fullStr |
Characteristics of microbial communities and intestinal pathogenic bacteria for migrated Larus ridibundus in southwest China |
title_full_unstemmed |
Characteristics of microbial communities and intestinal pathogenic bacteria for migrated Larus ridibundus in southwest China |
title_sort |
characteristics of microbial communities and intestinal pathogenic bacteria for migrated larus ridibundus in southwest china |
publisher |
Wiley |
series |
MicrobiologyOpen |
issn |
2045-8827 |
publishDate |
2019-04-01 |
description |
Abstract Larus ridibundus, a migratory wild bird, has become one of the most popular gull species in southwest China in recent years. There has been no information on the gut microbiota and intestinal pathogenic bacteria configuration in wild L. ridibundus, even though the public are in close contact with this bird. In this study, 16S rRNA amplicon‐sequencing methods were used to describe the microbial community structure and intestinal pathogenic bacteria were isolated to identify their characteristics. The taxonomic results revealed that Firmicutes (86%), Proteobacteria (10%), and Tenericutes (3%) were the three most abundant phyla in the gut of L. ridibundus. Enterococcaceae, Enterobacteriaceae, and Mycoplasmataceae were the most predominant families, respectively. The number of operational taxonomic units (OTUs), the richness estimates and diversity indices of microbiota, was statistically different (p < 0.05). However, beta diversity showed that no statistical significance (p > 0.05) between all the fecal samples. The most frequently isolated intestinal pathogenic bacteria from L. ridibundus were enteropathogenic Escherichia coli (32%) and Salmonella (21%). Pulsed‐field gel electrophoresis (PFGE) results of Salmonella species revealed a high degree of similarity between isolates, which was not observed for other species. None of the potentially pathogenic isolates were identical to human‐isolated counterparts suggesting that there was little cross‐infection between humans and gulls, despite close proximity. In brief, this study provided a baseline for future L. ridibundus microbiology analysis, and made an understanding of the intestinal bacterial community structure and diversity. |
topic |
gut microbiota intestinal pathogenic bacteria Larus ridibundus southwest China |
url |
https://doi.org/10.1002/mbo3.693 |
work_keys_str_mv |
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spelling |
doaj-f0942c51c62f46e38780ac55c53a87f32020-11-25T00:53:57ZengWileyMicrobiologyOpen2045-88272019-04-0184n/an/a10.1002/mbo3.693Characteristics of microbial communities and intestinal pathogenic bacteria for migrated Larus ridibundus in southwest ChinaFeng Liao0Wenpeng Gu1Duo Li2Junrong Liang3Xiaoqing Fu4Wen Xu5Ran Duan6Xin Wang7Huaiqi Jing8Jiejie Dai9Department of Respiratory Medicine The First People's Hospital of Yunnan Province Kunming Yunnan ChinaCenter of Tree Shrew Germplasm Resources Institute of Medical Biology The Chinese Academy of Medical Science and Peking Union Medical College Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases Yunnan Innovation Team of Standardization and Application Research in Tree Shrew Kunming ChinaDepartment of Acute Infectious Diseases Control and Prevention Yunnan Provincial Centre for Disease Control and Prevention Kunming ChinaNational Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention State Key Laboratory for Infectious Disease Prevention and Control Beijing ChinaDepartment of Acute Infectious Diseases Control and Prevention Yunnan Provincial Centre for Disease Control and Prevention Kunming ChinaDepartment of Acute Infectious Diseases Control and Prevention Yunnan Provincial Centre for Disease Control and Prevention Kunming ChinaNational Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention State Key Laboratory for Infectious Disease Prevention and Control Beijing ChinaNational Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention State Key Laboratory for Infectious Disease Prevention and Control Beijing ChinaNational Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention State Key Laboratory for Infectious Disease Prevention and Control Beijing ChinaCenter of Tree Shrew Germplasm Resources Institute of Medical Biology The Chinese Academy of Medical Science and Peking Union Medical College Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases Yunnan Innovation Team of Standardization and Application Research in Tree Shrew Kunming ChinaAbstract Larus ridibundus, a migratory wild bird, has become one of the most popular gull species in southwest China in recent years. There has been no information on the gut microbiota and intestinal pathogenic bacteria configuration in wild L. ridibundus, even though the public are in close contact with this bird. In this study, 16S rRNA amplicon‐sequencing methods were used to describe the microbial community structure and intestinal pathogenic bacteria were isolated to identify their characteristics. The taxonomic results revealed that Firmicutes (86%), Proteobacteria (10%), and Tenericutes (3%) were the three most abundant phyla in the gut of L. ridibundus. Enterococcaceae, Enterobacteriaceae, and Mycoplasmataceae were the most predominant families, respectively. The number of operational taxonomic units (OTUs), the richness estimates and diversity indices of microbiota, was statistically different (p < 0.05). However, beta diversity showed that no statistical significance (p > 0.05) between all the fecal samples. The most frequently isolated intestinal pathogenic bacteria from L. ridibundus were enteropathogenic Escherichia coli (32%) and Salmonella (21%). Pulsed‐field gel electrophoresis (PFGE) results of Salmonella species revealed a high degree of similarity between isolates, which was not observed for other species. None of the potentially pathogenic isolates were identical to human‐isolated counterparts suggesting that there was little cross‐infection between humans and gulls, despite close proximity. In brief, this study provided a baseline for future L. ridibundus microbiology analysis, and made an understanding of the intestinal bacterial community structure and diversity.https://doi.org/10.1002/mbo3.693gut microbiotaintestinal pathogenic bacteriaLarus ridibundussouthwest China |