Expression and Functional Characterization of <i>c-Fos</i> Gene in Chinese Fire-Bellied Newt <i>Cynops Orientalis</i>
<i>c-Fos</i> is an immediate-early gene that modulates cellular responses to a wide variety of stimuli and also plays an important role in tissue regeneration. However, the sequence and functions of <i>c-Fos</i> are still poorly understood in newts. This study describes the m...
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doaj-f0961822d2f94ae0bcc7a2d36e883b282021-01-31T00:04:25ZengMDPI AGGenes2073-44252021-01-011220520510.3390/genes12020205Expression and Functional Characterization of <i>c-Fos</i> Gene in Chinese Fire-Bellied Newt <i>Cynops Orientalis</i>Gang Ye0Yalong Feng1Zhaoxiang Mi2Du Wang3Shuai Lin4Fulin Chen5Jihong Cui6Yuan Yu7Lab of Tissue Engineering, College of Life Sciences, Northwest University, Xi’an 710069, ChinaLab of Tissue Engineering, College of Life Sciences, Northwest University, Xi’an 710069, ChinaLab of Tissue Engineering, College of Life Sciences, Northwest University, Xi’an 710069, ChinaLab of Tissue Engineering, College of Life Sciences, Northwest University, Xi’an 710069, ChinaLab of Tissue Engineering, College of Life Sciences, Northwest University, Xi’an 710069, ChinaLab of Tissue Engineering, College of Life Sciences, Northwest University, Xi’an 710069, ChinaLab of Tissue Engineering, College of Life Sciences, Northwest University, Xi’an 710069, ChinaLab of Tissue Engineering, College of Life Sciences, Northwest University, Xi’an 710069, China<i>c-Fos</i> is an immediate-early gene that modulates cellular responses to a wide variety of stimuli and also plays an important role in tissue regeneration. However, the sequence and functions of <i>c-Fos</i> are still poorly understood in newts. This study describes the molecular cloning and characterization of the <i>c-Fos</i> gene (<i>Co-c-Fos</i>) of the Chinese fire-bellied newt, <i>Cynops orientalis</i>. The full-length <i>Co-c-Fos</i> cDNA sequence consists of a 1290 bp coding sequence that encoded 429 amino acids. The alignment and phylogenetic analyses reveal that the amino acid sequence of <i>Co-c-Fos</i> shared a conserved basic leucine zipper domain, including a nuclear localization sequence and a leucine heptad repeat. The <i>Co-c-Fos</i> mRNA is widely expressed in various tissues and is highly and uniformly expressed along the newt limb. After limb amputation, the expression of <i>Co-c-Fos</i> mRNA was immediately upregulated, but rapidly declined. However, the significant upregulation of Co-c-Fos protein expression was sustained for 24 h, overlapping with the wound healing stage of <i>C. orientalis</i> limb regeneration. To investigate if <i>Co-c-Fos</i> participate in newt wound healing, a skin wound healing model is employed. The results show that the treatment of T-5224, a selective c-Fos inhibitor, could largely impair the healing process of newt’s skin wound, as well as the injury-induced <i>matrix metalloproteinase-3</i> upregulation, which is fundamental to wound epithelium formation. These data suggest that <i>Co-c-Fos</i> might participate in wound healing by modulating the expression of its potential target gene <i>matrix metalloproteinase-3</i>. Our study provides important insights into mechanisms that are responsible for the initiation of newt limb regeneration.https://www.mdpi.com/2073-4425/12/2/205<i>Cynops orientalis</i><i>c-Fos</i>expression patternslimb regenerationskin wound healing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gang Ye Yalong Feng Zhaoxiang Mi Du Wang Shuai Lin Fulin Chen Jihong Cui Yuan Yu |
spellingShingle |
Gang Ye Yalong Feng Zhaoxiang Mi Du Wang Shuai Lin Fulin Chen Jihong Cui Yuan Yu Expression and Functional Characterization of <i>c-Fos</i> Gene in Chinese Fire-Bellied Newt <i>Cynops Orientalis</i> Genes <i>Cynops orientalis</i> <i>c-Fos</i> expression patterns limb regeneration skin wound healing |
author_facet |
Gang Ye Yalong Feng Zhaoxiang Mi Du Wang Shuai Lin Fulin Chen Jihong Cui Yuan Yu |
author_sort |
Gang Ye |
title |
Expression and Functional Characterization of <i>c-Fos</i> Gene in Chinese Fire-Bellied Newt <i>Cynops Orientalis</i> |
title_short |
Expression and Functional Characterization of <i>c-Fos</i> Gene in Chinese Fire-Bellied Newt <i>Cynops Orientalis</i> |
title_full |
Expression and Functional Characterization of <i>c-Fos</i> Gene in Chinese Fire-Bellied Newt <i>Cynops Orientalis</i> |
title_fullStr |
Expression and Functional Characterization of <i>c-Fos</i> Gene in Chinese Fire-Bellied Newt <i>Cynops Orientalis</i> |
title_full_unstemmed |
Expression and Functional Characterization of <i>c-Fos</i> Gene in Chinese Fire-Bellied Newt <i>Cynops Orientalis</i> |
title_sort |
expression and functional characterization of <i>c-fos</i> gene in chinese fire-bellied newt <i>cynops orientalis</i> |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2021-01-01 |
description |
<i>c-Fos</i> is an immediate-early gene that modulates cellular responses to a wide variety of stimuli and also plays an important role in tissue regeneration. However, the sequence and functions of <i>c-Fos</i> are still poorly understood in newts. This study describes the molecular cloning and characterization of the <i>c-Fos</i> gene (<i>Co-c-Fos</i>) of the Chinese fire-bellied newt, <i>Cynops orientalis</i>. The full-length <i>Co-c-Fos</i> cDNA sequence consists of a 1290 bp coding sequence that encoded 429 amino acids. The alignment and phylogenetic analyses reveal that the amino acid sequence of <i>Co-c-Fos</i> shared a conserved basic leucine zipper domain, including a nuclear localization sequence and a leucine heptad repeat. The <i>Co-c-Fos</i> mRNA is widely expressed in various tissues and is highly and uniformly expressed along the newt limb. After limb amputation, the expression of <i>Co-c-Fos</i> mRNA was immediately upregulated, but rapidly declined. However, the significant upregulation of Co-c-Fos protein expression was sustained for 24 h, overlapping with the wound healing stage of <i>C. orientalis</i> limb regeneration. To investigate if <i>Co-c-Fos</i> participate in newt wound healing, a skin wound healing model is employed. The results show that the treatment of T-5224, a selective c-Fos inhibitor, could largely impair the healing process of newt’s skin wound, as well as the injury-induced <i>matrix metalloproteinase-3</i> upregulation, which is fundamental to wound epithelium formation. These data suggest that <i>Co-c-Fos</i> might participate in wound healing by modulating the expression of its potential target gene <i>matrix metalloproteinase-3</i>. Our study provides important insights into mechanisms that are responsible for the initiation of newt limb regeneration. |
topic |
<i>Cynops orientalis</i> <i>c-Fos</i> expression patterns limb regeneration skin wound healing |
url |
https://www.mdpi.com/2073-4425/12/2/205 |
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