Major constituents of Cistanche tubulosa, echinacoside and acteoside, inhibit sodium-dependent glucose cotransporter 1-mediated glucose uptake by intestinal epithelial cells
Echinacoside (ECH) and acteoside (ACT), the major constituents of Cistanche tubulosa, suppress the increase in postprandial blood glucose level. Although ECH and ACT have been reported to weakly inhibit α-glucosidases, the underlying mechanism remains unclear. Therefore, we focused on the regulatory...
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doaj-f0974283c15b499d9c9ec4836a80de662021-04-30T07:12:00ZengElsevierJournal of Functional Foods1756-46462017-12-01399195Major constituents of Cistanche tubulosa, echinacoside and acteoside, inhibit sodium-dependent glucose cotransporter 1-mediated glucose uptake by intestinal epithelial cellsHiroaki Shimada0Yuichi Urabe1Yuhei Okamoto2Zheng Li3Atsushi Kawase4Toshio Morikawa5Pengfei Tu6Osamu Muraoka7Masahiro Iwaki8Department of Pharmacy, Faculty of Pharmacy, Kindai University, Kowakae, Higashi-osaka 577-8502, JapanDepartment of Pharmacy, Faculty of Pharmacy, Kindai University, Kowakae, Higashi-osaka 577-8502, JapanDepartment of Pharmacy, Faculty of Pharmacy, Kindai University, Kowakae, Higashi-osaka 577-8502, JapanEishin Pharmaceutical Co., Ltd., 2-9-1 Higashi-tenma, Kita-ku, Osaka 530-0044, JapanDepartment of Pharmacy, Faculty of Pharmacy, Kindai University, Kowakae, Higashi-osaka 577-8502, JapanPharmaceutical Research and Technology Institute, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka 577-8502, Japan; Antiaging Center, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka 577-8502, JapanModern Research Centre for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, ChinaDepartment of Pharmacy, Faculty of Pharmacy, Kindai University, Kowakae, Higashi-osaka 577-8502, Japan; Pharmaceutical Research and Technology Institute, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka 577-8502, Japan; Antiaging Center, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka 577-8502, JapanDepartment of Pharmacy, Faculty of Pharmacy, Kindai University, Kowakae, Higashi-osaka 577-8502, Japan; Pharmaceutical Research and Technology Institute, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka 577-8502, Japan; Antiaging Center, Kindai University, 3-4-1 Kowakae, Higashi-osaka, Osaka 577-8502, Japan; Corresponding author at: Department of Pharmacy, Faculty of Pharmacy, Kindai University, Kowakae, Higashi-osaka 577-8502, Japan.Echinacoside (ECH) and acteoside (ACT), the major constituents of Cistanche tubulosa, suppress the increase in postprandial blood glucose level. Although ECH and ACT have been reported to weakly inhibit α-glucosidases, the underlying mechanism remains unclear. Therefore, we focused on the regulatory mechanism of dietary glucose absorption. In this study, we aimed to clarify the inhibitory effects of ECH and ACT on sodium-dependent glucose cotransporter (SGLT) 1-mediated gastrointestinal glucose absorption. Uptake experiments were performed using human intestinal Caco-2 cells and the fluorescence glucose analogue, 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-d-glucose (2-NBDG). Sodium-dependent 2-NBDG uptake was successfully estimated and this uptake was completely inhibited by an SGLT inhibitor phlorizin. ECH and ACT inhibited sodium-dependent 2-NBDG uptake in a concentration-dependent manner. However, this inhibition was not observed under sodium-free condition. This study suggested that the inhibitory effects of ECH and ACT on SGLT1-mediated glucose uptake contribute to suppression of increased postprandial blood glucose level.http://www.sciencedirect.com/science/article/pii/S1756464617305996Cistanche tubulosaSodium-dependent glucose cotransporter 1Glucose uptakeTraditional Chinese medicineAntidiabetic agent |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hiroaki Shimada Yuichi Urabe Yuhei Okamoto Zheng Li Atsushi Kawase Toshio Morikawa Pengfei Tu Osamu Muraoka Masahiro Iwaki |
spellingShingle |
Hiroaki Shimada Yuichi Urabe Yuhei Okamoto Zheng Li Atsushi Kawase Toshio Morikawa Pengfei Tu Osamu Muraoka Masahiro Iwaki Major constituents of Cistanche tubulosa, echinacoside and acteoside, inhibit sodium-dependent glucose cotransporter 1-mediated glucose uptake by intestinal epithelial cells Journal of Functional Foods Cistanche tubulosa Sodium-dependent glucose cotransporter 1 Glucose uptake Traditional Chinese medicine Antidiabetic agent |
author_facet |
Hiroaki Shimada Yuichi Urabe Yuhei Okamoto Zheng Li Atsushi Kawase Toshio Morikawa Pengfei Tu Osamu Muraoka Masahiro Iwaki |
author_sort |
Hiroaki Shimada |
title |
Major constituents of Cistanche tubulosa, echinacoside and acteoside, inhibit sodium-dependent glucose cotransporter 1-mediated glucose uptake by intestinal epithelial cells |
title_short |
Major constituents of Cistanche tubulosa, echinacoside and acteoside, inhibit sodium-dependent glucose cotransporter 1-mediated glucose uptake by intestinal epithelial cells |
title_full |
Major constituents of Cistanche tubulosa, echinacoside and acteoside, inhibit sodium-dependent glucose cotransporter 1-mediated glucose uptake by intestinal epithelial cells |
title_fullStr |
Major constituents of Cistanche tubulosa, echinacoside and acteoside, inhibit sodium-dependent glucose cotransporter 1-mediated glucose uptake by intestinal epithelial cells |
title_full_unstemmed |
Major constituents of Cistanche tubulosa, echinacoside and acteoside, inhibit sodium-dependent glucose cotransporter 1-mediated glucose uptake by intestinal epithelial cells |
title_sort |
major constituents of cistanche tubulosa, echinacoside and acteoside, inhibit sodium-dependent glucose cotransporter 1-mediated glucose uptake by intestinal epithelial cells |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2017-12-01 |
description |
Echinacoside (ECH) and acteoside (ACT), the major constituents of Cistanche tubulosa, suppress the increase in postprandial blood glucose level. Although ECH and ACT have been reported to weakly inhibit α-glucosidases, the underlying mechanism remains unclear. Therefore, we focused on the regulatory mechanism of dietary glucose absorption. In this study, we aimed to clarify the inhibitory effects of ECH and ACT on sodium-dependent glucose cotransporter (SGLT) 1-mediated gastrointestinal glucose absorption. Uptake experiments were performed using human intestinal Caco-2 cells and the fluorescence glucose analogue, 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-d-glucose (2-NBDG). Sodium-dependent 2-NBDG uptake was successfully estimated and this uptake was completely inhibited by an SGLT inhibitor phlorizin. ECH and ACT inhibited sodium-dependent 2-NBDG uptake in a concentration-dependent manner. However, this inhibition was not observed under sodium-free condition. This study suggested that the inhibitory effects of ECH and ACT on SGLT1-mediated glucose uptake contribute to suppression of increased postprandial blood glucose level. |
topic |
Cistanche tubulosa Sodium-dependent glucose cotransporter 1 Glucose uptake Traditional Chinese medicine Antidiabetic agent |
url |
http://www.sciencedirect.com/science/article/pii/S1756464617305996 |
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