Optimization of Alkaline Extraction of Polysaccharides from Ganoderma lucidum and Their Effect on Immune Function in Mice

Optimization of Alkaline Extraction of Polysaccharides from Ganoderma lucidum and Their Effect on Immune Function in Mice

Response surface methodology was employed to optimize the conditions for alkaline extraction of polysaccharides from Ganoderma lucidum. The results indicated that the optimum conditions were an extraction temperature of 60.1 ºC, an extraction time of 77.3 min, a sodium hydroxide (NaOH) concentration...

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Main Authors: Sheng-Quan Huang, Jin-Wei Li, Zhou Wang, Hua-Xin Pan, Jiang-Xu Chen, Zheng-Xiang Ning
Format: Article
Language:English
Published: MDPI AG 2010-05-01
Series:Molecules
Subjects:
Ganoderma lucidum
alkaline extraction
polysaccharides
yield
immune activity
Online Access:http://www.mdpi.com/1420-3049/15/5/3694/
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spelling doaj-f0988a1a864249d0b421eba9ac0de5b22020-11-24T23:48:08ZengMDPI AGMolecules1420-30492010-05-011553694370810.3390/molecules15053694Optimization of Alkaline Extraction of Polysaccharides from Ganoderma lucidum and Their Effect on Immune Function in MiceSheng-Quan HuangJin-Wei LiZhou WangHua-Xin PanJiang-Xu ChenZheng-Xiang NingResponse surface methodology was employed to optimize the conditions for alkaline extraction of polysaccharides from Ganoderma lucidum. The results indicated that the optimum conditions were an extraction temperature of 60.1 ºC, an extraction time of 77.3 min, a sodium hydroxide (NaOH) concentration of 5.1% and a substrate/liquid ratio of 1:21.4. Immunological assays results have shown that the alkaline soluble polysaccharides have no noticeable effects on monocyte phagocytosis and immune organ (spleen, thymus) weight of of immunocompromised mice at the tested dosages. However, they could restore delayed type hypersensitivity reaction to dinitrofluorobenzene (DFNB), hemolysis antibody levels at the three doses applied, and improve the natural killer cell activity at the high-dose and medium dose. http://www.mdpi.com/1420-3049/15/5/3694/Ganoderma lucidumalkaline extractionpolysaccharidesyieldimmune activity
collection DOAJ
language English
format Article
sources DOAJ
author Sheng-Quan Huang
Jin-Wei Li
Zhou Wang
Hua-Xin Pan
Jiang-Xu Chen
Zheng-Xiang Ning
spellingShingle Sheng-Quan Huang
Jin-Wei Li
Zhou Wang
Hua-Xin Pan
Jiang-Xu Chen
Zheng-Xiang Ning
Optimization of Alkaline Extraction of Polysaccharides from Ganoderma lucidum and Their Effect on Immune Function in Mice
Molecules
Ganoderma lucidum
alkaline extraction
polysaccharides
yield
immune activity
author_facet Sheng-Quan Huang
Jin-Wei Li
Zhou Wang
Hua-Xin Pan
Jiang-Xu Chen
Zheng-Xiang Ning
author_sort Sheng-Quan Huang
title Optimization of Alkaline Extraction of Polysaccharides from Ganoderma lucidum and Their Effect on Immune Function in Mice
title_short Optimization of Alkaline Extraction of Polysaccharides from Ganoderma lucidum and Their Effect on Immune Function in Mice
title_full Optimization of Alkaline Extraction of Polysaccharides from Ganoderma lucidum and Their Effect on Immune Function in Mice
title_fullStr Optimization of Alkaline Extraction of Polysaccharides from Ganoderma lucidum and Their Effect on Immune Function in Mice
title_full_unstemmed Optimization of Alkaline Extraction of Polysaccharides from Ganoderma lucidum and Their Effect on Immune Function in Mice
title_sort optimization of alkaline extraction of polysaccharides from ganoderma lucidum and their effect on immune function in mice
publisher MDPI AG
series Molecules
issn 1420-3049
publishDate 2010-05-01
description Response surface methodology was employed to optimize the conditions for alkaline extraction of polysaccharides from Ganoderma lucidum. The results indicated that the optimum conditions were an extraction temperature of 60.1 ºC, an extraction time of 77.3 min, a sodium hydroxide (NaOH) concentration of 5.1% and a substrate/liquid ratio of 1:21.4. Immunological assays results have shown that the alkaline soluble polysaccharides have no noticeable effects on monocyte phagocytosis and immune organ (spleen, thymus) weight of of immunocompromised mice at the tested dosages. However, they could restore delayed type hypersensitivity reaction to dinitrofluorobenzene (DFNB), hemolysis antibody levels at the three doses applied, and improve the natural killer cell activity at the high-dose and medium dose.
topic Ganoderma lucidum
alkaline extraction
polysaccharides
yield
immune activity
url http://www.mdpi.com/1420-3049/15/5/3694/
work_keys_str_mv AT shengquanhuang optimizationofalkalineextractionofpolysaccharidesfromganodermalucidumandtheireffectonimmunefunctioninmice
AT jinweili optimizationofalkalineextractionofpolysaccharidesfromganodermalucidumandtheireffectonimmunefunctioninmice
AT zhouwang optimizationofalkalineextractionofpolysaccharidesfromganodermalucidumandtheireffectonimmunefunctioninmice
AT huaxinpan optimizationofalkalineextractionofpolysaccharidesfromganodermalucidumandtheireffectonimmunefunctioninmice
AT jiangxuchen optimizationofalkalineextractionofpolysaccharidesfromganodermalucidumandtheireffectonimmunefunctioninmice
AT zhengxiangning optimizationofalkalineextractionofpolysaccharidesfromganodermalucidumandtheireffectonimmunefunctioninmice
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