The effects of cigarette smoke extract on ovulation, oocyte morphology and ovarian gene expression in mice.
Cigarette smoking can harm fertility, but the existing research has targeted primarily on ovarian follicles, embryos or sex hormone. In this study, we tested cigarette smoke extract on ovulation, oocyte morphology and ovarian gene expression associated with inhibition of oxidative stress using C57BL...
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doaj-f0bcbebeb6f34af18291329eba29091a2020-11-25T00:47:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9594510.1371/journal.pone.0095945The effects of cigarette smoke extract on ovulation, oocyte morphology and ovarian gene expression in mice.Zixin MaiMing LeiBolan YuHongzi DuJianqiao LiuCigarette smoking can harm fertility, but the existing research has targeted primarily on ovarian follicles, embryos or sex hormone. In this study, we tested cigarette smoke extract on ovulation, oocyte morphology and ovarian gene expression associated with inhibition of oxidative stress using C57BL/6 mice. Mice in the experimental group were administered a cigarette smoke extract (CSE) solution (2 mg/ml) orally daily, while the blank control group was given dimethylsulfoxide (DMSO). A positive control group (menadione) was used that received an intraperitoneal injection of 15 mg/kg menadione in oil solution daily. We found that the CSE group manifested a reduced diameter of zona pellucida-free oocyte (ZP-free OD) and a morphologically misshapen first polar body (PB). Our results suggest that CSE exposure is associated with a shrink size and poor quality of oocytes. Quitting smoking is a wise choice to ensure good fertility.http://europepmc.org/articles/PMC4002431?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zixin Mai Ming Lei Bolan Yu Hongzi Du Jianqiao Liu |
spellingShingle |
Zixin Mai Ming Lei Bolan Yu Hongzi Du Jianqiao Liu The effects of cigarette smoke extract on ovulation, oocyte morphology and ovarian gene expression in mice. PLoS ONE |
author_facet |
Zixin Mai Ming Lei Bolan Yu Hongzi Du Jianqiao Liu |
author_sort |
Zixin Mai |
title |
The effects of cigarette smoke extract on ovulation, oocyte morphology and ovarian gene expression in mice. |
title_short |
The effects of cigarette smoke extract on ovulation, oocyte morphology and ovarian gene expression in mice. |
title_full |
The effects of cigarette smoke extract on ovulation, oocyte morphology and ovarian gene expression in mice. |
title_fullStr |
The effects of cigarette smoke extract on ovulation, oocyte morphology and ovarian gene expression in mice. |
title_full_unstemmed |
The effects of cigarette smoke extract on ovulation, oocyte morphology and ovarian gene expression in mice. |
title_sort |
effects of cigarette smoke extract on ovulation, oocyte morphology and ovarian gene expression in mice. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Cigarette smoking can harm fertility, but the existing research has targeted primarily on ovarian follicles, embryos or sex hormone. In this study, we tested cigarette smoke extract on ovulation, oocyte morphology and ovarian gene expression associated with inhibition of oxidative stress using C57BL/6 mice. Mice in the experimental group were administered a cigarette smoke extract (CSE) solution (2 mg/ml) orally daily, while the blank control group was given dimethylsulfoxide (DMSO). A positive control group (menadione) was used that received an intraperitoneal injection of 15 mg/kg menadione in oil solution daily. We found that the CSE group manifested a reduced diameter of zona pellucida-free oocyte (ZP-free OD) and a morphologically misshapen first polar body (PB). Our results suggest that CSE exposure is associated with a shrink size and poor quality of oocytes. Quitting smoking is a wise choice to ensure good fertility. |
url |
http://europepmc.org/articles/PMC4002431?pdf=render |
work_keys_str_mv |
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