Identification of Novel Fusion Transcripts in High Grade Serous Ovarian Cancer
Fusion genes are structural chromosomal rearrangements resulting in the exchange of DNA sequences between genes. This results in the formation of a new combined gene. They have been implicated in carcinogenesis in a number of different cancers, though they have been understudied in high grade serous...
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doaj-f0bfb56621d44b5ea72c399b9207c0362021-04-30T23:05:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-04-01224791479110.3390/ijms22094791Identification of Novel Fusion Transcripts in High Grade Serous Ovarian CancerAndreea Newtson0Henry Reyes1Eric J. Devor2Michael J. Goodheart3Jesus Gonzalez Bosquet4Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, University of Iowa Hospitals and Clinics, Iowa City, IA 52242, USADepartment of Obstetrics and Gynecology, University of Buffalo, Buffalo, NY 14260, USAHolden Comprehensive Cancer Center, University of Iowa Hospitals and Clinics, Iowa City, IA 52242, USADepartment of Obstetrics and Gynecology, Division of Gynecologic Oncology, University of Iowa Hospitals and Clinics, Iowa City, IA 52242, USADepartment of Obstetrics and Gynecology, Division of Gynecologic Oncology, University of Iowa Hospitals and Clinics, Iowa City, IA 52242, USAFusion genes are structural chromosomal rearrangements resulting in the exchange of DNA sequences between genes. This results in the formation of a new combined gene. They have been implicated in carcinogenesis in a number of different cancers, though they have been understudied in high grade serous ovarian cancer. This study used high throughput tools to compare the transcriptome of high grade serous ovarian cancer and normal fallopian tubes in the interest of identifying unique fusion transcripts within each group. Indeed, we found that there were significantly more fusion transcripts in the cancer samples relative to the normal fallopian tubes. Following this, the role of fusion transcripts in chemo-response and overall survival was investigated. This led to the identification of fusion transcripts significantly associated with overall survival. Validation was performed with different analytical platforms and different algorithms to find fusion transcripts.https://www.mdpi.com/1422-0067/22/9/4791fusion genesfusion transcriptshigh grade serous ovarian cancerwhole transcriptome sequencing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Andreea Newtson Henry Reyes Eric J. Devor Michael J. Goodheart Jesus Gonzalez Bosquet |
spellingShingle |
Andreea Newtson Henry Reyes Eric J. Devor Michael J. Goodheart Jesus Gonzalez Bosquet Identification of Novel Fusion Transcripts in High Grade Serous Ovarian Cancer International Journal of Molecular Sciences fusion genes fusion transcripts high grade serous ovarian cancer whole transcriptome sequencing |
author_facet |
Andreea Newtson Henry Reyes Eric J. Devor Michael J. Goodheart Jesus Gonzalez Bosquet |
author_sort |
Andreea Newtson |
title |
Identification of Novel Fusion Transcripts in High Grade Serous Ovarian Cancer |
title_short |
Identification of Novel Fusion Transcripts in High Grade Serous Ovarian Cancer |
title_full |
Identification of Novel Fusion Transcripts in High Grade Serous Ovarian Cancer |
title_fullStr |
Identification of Novel Fusion Transcripts in High Grade Serous Ovarian Cancer |
title_full_unstemmed |
Identification of Novel Fusion Transcripts in High Grade Serous Ovarian Cancer |
title_sort |
identification of novel fusion transcripts in high grade serous ovarian cancer |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-04-01 |
description |
Fusion genes are structural chromosomal rearrangements resulting in the exchange of DNA sequences between genes. This results in the formation of a new combined gene. They have been implicated in carcinogenesis in a number of different cancers, though they have been understudied in high grade serous ovarian cancer. This study used high throughput tools to compare the transcriptome of high grade serous ovarian cancer and normal fallopian tubes in the interest of identifying unique fusion transcripts within each group. Indeed, we found that there were significantly more fusion transcripts in the cancer samples relative to the normal fallopian tubes. Following this, the role of fusion transcripts in chemo-response and overall survival was investigated. This led to the identification of fusion transcripts significantly associated with overall survival. Validation was performed with different analytical platforms and different algorithms to find fusion transcripts. |
topic |
fusion genes fusion transcripts high grade serous ovarian cancer whole transcriptome sequencing |
url |
https://www.mdpi.com/1422-0067/22/9/4791 |
work_keys_str_mv |
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