Antimicrobial Resistance and Whole-Genome Characterisation of High-Level Ciprofloxacin-Resistant <i>Salmonella</i> Enterica Serovar Kentucky ST 198 Strains Isolated from Human in Poland
Background: <i>Salmonella</i> Kentucky belongs to zoonotic serotypes that demonstrate that the high antimicrobial resistance and multidrug resistance (including fluoroquinolones) is an emerging problem. To the best of our knowledge, clinical <i>S.</i> Kentucky strains isolate...
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doaj-f0cdd2652cf1472e8700b3aeafbb52652021-09-09T13:47:48ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-01229381938110.3390/ijms22179381Antimicrobial Resistance and Whole-Genome Characterisation of High-Level Ciprofloxacin-Resistant <i>Salmonella</i> Enterica Serovar Kentucky ST 198 Strains Isolated from Human in PolandTomasz Wołkowicz0Katarzyna Zacharczuk1Rafał Gierczyński2Magdalena Nowakowska3Katarzyna Piekarska4Department of Bacteriology and Biocontamination Control, National Institute of Public Health NIH—National Research Institute, 00-791 Warsaw, PolandDepartment of Bacteriology and Biocontamination Control, National Institute of Public Health NIH—National Research Institute, 00-791 Warsaw, PolandDepartment of Bacteriology and Biocontamination Control, National Institute of Public Health NIH—National Research Institute, 00-791 Warsaw, PolandDepartment of Bacteriology and Biocontamination Control, National Institute of Public Health NIH—National Research Institute, 00-791 Warsaw, PolandDepartment of Bacteriology and Biocontamination Control, National Institute of Public Health NIH—National Research Institute, 00-791 Warsaw, PolandBackground: <i>Salmonella</i> Kentucky belongs to zoonotic serotypes that demonstrate that the high antimicrobial resistance and multidrug resistance (including fluoroquinolones) is an emerging problem. To the best of our knowledge, clinical <i>S.</i> Kentucky strains isolated in Poland remain undescribed. Methods: Eighteen clinical <i>S.</i> Kentucky strains collected in the years 2018–2019 in Poland were investigated. All the strains were tested for susceptibility to 11 antimicrobials using the disc diffusion and E-test methods. Whole genome sequences were analysed for antimicrobial resistance genes, mutations, the presence and structure of SGI1-K (Salmonella Genomic Island and the genetic relationship of the isolates. Results: Sixteen of 18 isolates (88.9%) were assigned as ST198 and were found to be high-level resistant to ampicillin (>256 mg/L) and quinolones (nalidixic acid MIC ≥ 1024 mg/L, ciprofloxacin MIC range 6–16 mg/L). All the 16 strains revealed three mutations in QRDR of GyrA and ParC. The substitutions of Ser83 → Phe and Asp87 → Tyr of the GyrA subunit and Ser80→Ile of the ParC subunit were the most common. One <i>S.</i> Kentucky isolate had <i>qnrS1</i> in addition to the QRDR mutations. Five of the ST198 strains, grouped in cluster A, had multiple resistant determinants like <i>bla</i>TEM1-B, <i>aac(6′)-Iaa</i>, <i>sul1</i> or <i>tetA</i>, mostly in SGI1 K. Seven strains, grouped in cluster B, had shorter SGI1-K with deletions of many regions and with few resistance genes detected. Conclusion: The results of this study demonstrated that a significant part of <i>S.</i> Kentucky isolates from humans in Poland belonged to ST198 and were high-level resistant to ampicillin and quinolones.https://www.mdpi.com/1422-0067/22/17/9381<i>Salmonella</i> Kentuckyantibiotic resistancemultidrug resistanceciprofloxacinST198 |
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language |
English |
format |
Article |
sources |
DOAJ |
author |
Tomasz Wołkowicz Katarzyna Zacharczuk Rafał Gierczyński Magdalena Nowakowska Katarzyna Piekarska |
spellingShingle |
Tomasz Wołkowicz Katarzyna Zacharczuk Rafał Gierczyński Magdalena Nowakowska Katarzyna Piekarska Antimicrobial Resistance and Whole-Genome Characterisation of High-Level Ciprofloxacin-Resistant <i>Salmonella</i> Enterica Serovar Kentucky ST 198 Strains Isolated from Human in Poland International Journal of Molecular Sciences <i>Salmonella</i> Kentucky antibiotic resistance multidrug resistance ciprofloxacin ST198 |
author_facet |
Tomasz Wołkowicz Katarzyna Zacharczuk Rafał Gierczyński Magdalena Nowakowska Katarzyna Piekarska |
author_sort |
Tomasz Wołkowicz |
title |
Antimicrobial Resistance and Whole-Genome Characterisation of High-Level Ciprofloxacin-Resistant <i>Salmonella</i> Enterica Serovar Kentucky ST 198 Strains Isolated from Human in Poland |
title_short |
Antimicrobial Resistance and Whole-Genome Characterisation of High-Level Ciprofloxacin-Resistant <i>Salmonella</i> Enterica Serovar Kentucky ST 198 Strains Isolated from Human in Poland |
title_full |
Antimicrobial Resistance and Whole-Genome Characterisation of High-Level Ciprofloxacin-Resistant <i>Salmonella</i> Enterica Serovar Kentucky ST 198 Strains Isolated from Human in Poland |
title_fullStr |
Antimicrobial Resistance and Whole-Genome Characterisation of High-Level Ciprofloxacin-Resistant <i>Salmonella</i> Enterica Serovar Kentucky ST 198 Strains Isolated from Human in Poland |
title_full_unstemmed |
Antimicrobial Resistance and Whole-Genome Characterisation of High-Level Ciprofloxacin-Resistant <i>Salmonella</i> Enterica Serovar Kentucky ST 198 Strains Isolated from Human in Poland |
title_sort |
antimicrobial resistance and whole-genome characterisation of high-level ciprofloxacin-resistant <i>salmonella</i> enterica serovar kentucky st 198 strains isolated from human in poland |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-08-01 |
description |
Background: <i>Salmonella</i> Kentucky belongs to zoonotic serotypes that demonstrate that the high antimicrobial resistance and multidrug resistance (including fluoroquinolones) is an emerging problem. To the best of our knowledge, clinical <i>S.</i> Kentucky strains isolated in Poland remain undescribed. Methods: Eighteen clinical <i>S.</i> Kentucky strains collected in the years 2018–2019 in Poland were investigated. All the strains were tested for susceptibility to 11 antimicrobials using the disc diffusion and E-test methods. Whole genome sequences were analysed for antimicrobial resistance genes, mutations, the presence and structure of SGI1-K (Salmonella Genomic Island and the genetic relationship of the isolates. Results: Sixteen of 18 isolates (88.9%) were assigned as ST198 and were found to be high-level resistant to ampicillin (>256 mg/L) and quinolones (nalidixic acid MIC ≥ 1024 mg/L, ciprofloxacin MIC range 6–16 mg/L). All the 16 strains revealed three mutations in QRDR of GyrA and ParC. The substitutions of Ser83 → Phe and Asp87 → Tyr of the GyrA subunit and Ser80→Ile of the ParC subunit were the most common. One <i>S.</i> Kentucky isolate had <i>qnrS1</i> in addition to the QRDR mutations. Five of the ST198 strains, grouped in cluster A, had multiple resistant determinants like <i>bla</i>TEM1-B, <i>aac(6′)-Iaa</i>, <i>sul1</i> or <i>tetA</i>, mostly in SGI1 K. Seven strains, grouped in cluster B, had shorter SGI1-K with deletions of many regions and with few resistance genes detected. Conclusion: The results of this study demonstrated that a significant part of <i>S.</i> Kentucky isolates from humans in Poland belonged to ST198 and were high-level resistant to ampicillin and quinolones. |
topic |
<i>Salmonella</i> Kentucky antibiotic resistance multidrug resistance ciprofloxacin ST198 |
url |
https://www.mdpi.com/1422-0067/22/17/9381 |
work_keys_str_mv |
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