Plasma Treatment of Fish Cells: The Importance of Defining Cell Culture Conditions in Comparative Studies
The present study provides the fundamental results for the treatment of marine organisms with cold atmospheric pressure plasma. In farmed fish, skin lesions may occur as a result of intensive fish farming. Cold atmospheric plasma offers promising medical potential in wound healing processes. Since t...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-03-01
|
Series: | Applied Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-3417/11/6/2534 |
id |
doaj-f0fa2bfcb6714f419ae448ce68b08eaf |
---|---|
record_format |
Article |
spelling |
doaj-f0fa2bfcb6714f419ae448ce68b08eaf2021-03-13T00:01:09ZengMDPI AGApplied Sciences2076-34172021-03-01112534253410.3390/app11062534Plasma Treatment of Fish Cells: The Importance of Defining Cell Culture Conditions in Comparative StudiesHenrike Rebl0Claudia Bergemann1Sebastian Rakers2Barbara Nebe3Alexander Rebl4Department of Cell Biology, Rostock University Medical Center, Schillingallee 69, 18057 Rostock, GermanyDepartment of Cell Biology, Rostock University Medical Center, Schillingallee 69, 18057 Rostock, GermanyFraunhofer-Research Institution for Marine Biotechnology and Cell Technology, Mönkhofer Weg 239a, 23562 Lübeck, GermanyDepartment of Cell Biology, Rostock University Medical Center, Schillingallee 69, 18057 Rostock, GermanyInstitute of Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, GermanyThe present study provides the fundamental results for the treatment of marine organisms with cold atmospheric pressure plasma. In farmed fish, skin lesions may occur as a result of intensive fish farming. Cold atmospheric plasma offers promising medical potential in wound healing processes. Since the underlying plasma-mediated mechanisms at the physical and cellular level are yet to be fully understood, we investigated the sensitivity of three fish cell lines to plasma treatment in comparison with mammalian cells. We varied (I) cell density, (II) culture medium, and (III) pyruvate concentration in the medium as experimental parameters. Depending on the experimental setup, the plasma treatment affected the viability of the different cell lines to varying degrees. We conclude that it is mandatory to use similar cell densities and an identical medium, or at least a medium with identical antioxidant capacity, when studying plasma effects on different cell lines. Altogether, fish cells showed a higher sensitivity towards plasma treatment than mammalian cells in most of our setups. These results should increase the understanding of the future treatment of fish.https://www.mdpi.com/2076-3417/11/6/2534cold atmospheric pressure plasmafish cellsviabilityplasma-activated liquids |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Henrike Rebl Claudia Bergemann Sebastian Rakers Barbara Nebe Alexander Rebl |
spellingShingle |
Henrike Rebl Claudia Bergemann Sebastian Rakers Barbara Nebe Alexander Rebl Plasma Treatment of Fish Cells: The Importance of Defining Cell Culture Conditions in Comparative Studies Applied Sciences cold atmospheric pressure plasma fish cells viability plasma-activated liquids |
author_facet |
Henrike Rebl Claudia Bergemann Sebastian Rakers Barbara Nebe Alexander Rebl |
author_sort |
Henrike Rebl |
title |
Plasma Treatment of Fish Cells: The Importance of Defining Cell Culture Conditions in Comparative Studies |
title_short |
Plasma Treatment of Fish Cells: The Importance of Defining Cell Culture Conditions in Comparative Studies |
title_full |
Plasma Treatment of Fish Cells: The Importance of Defining Cell Culture Conditions in Comparative Studies |
title_fullStr |
Plasma Treatment of Fish Cells: The Importance of Defining Cell Culture Conditions in Comparative Studies |
title_full_unstemmed |
Plasma Treatment of Fish Cells: The Importance of Defining Cell Culture Conditions in Comparative Studies |
title_sort |
plasma treatment of fish cells: the importance of defining cell culture conditions in comparative studies |
publisher |
MDPI AG |
series |
Applied Sciences |
issn |
2076-3417 |
publishDate |
2021-03-01 |
description |
The present study provides the fundamental results for the treatment of marine organisms with cold atmospheric pressure plasma. In farmed fish, skin lesions may occur as a result of intensive fish farming. Cold atmospheric plasma offers promising medical potential in wound healing processes. Since the underlying plasma-mediated mechanisms at the physical and cellular level are yet to be fully understood, we investigated the sensitivity of three fish cell lines to plasma treatment in comparison with mammalian cells. We varied (I) cell density, (II) culture medium, and (III) pyruvate concentration in the medium as experimental parameters. Depending on the experimental setup, the plasma treatment affected the viability of the different cell lines to varying degrees. We conclude that it is mandatory to use similar cell densities and an identical medium, or at least a medium with identical antioxidant capacity, when studying plasma effects on different cell lines. Altogether, fish cells showed a higher sensitivity towards plasma treatment than mammalian cells in most of our setups. These results should increase the understanding of the future treatment of fish. |
topic |
cold atmospheric pressure plasma fish cells viability plasma-activated liquids |
url |
https://www.mdpi.com/2076-3417/11/6/2534 |
work_keys_str_mv |
AT henrikerebl plasmatreatmentoffishcellstheimportanceofdefiningcellcultureconditionsincomparativestudies AT claudiabergemann plasmatreatmentoffishcellstheimportanceofdefiningcellcultureconditionsincomparativestudies AT sebastianrakers plasmatreatmentoffishcellstheimportanceofdefiningcellcultureconditionsincomparativestudies AT barbaranebe plasmatreatmentoffishcellstheimportanceofdefiningcellcultureconditionsincomparativestudies AT alexanderrebl plasmatreatmentoffishcellstheimportanceofdefiningcellcultureconditionsincomparativestudies |
_version_ |
1724222640012394496 |