The dynamic changes of X chromosome inactivation during early culture of human embryonic stem cells
X chromosome inactivation (XCI) is required for dosage compensation of X-linked genes in human female cells. Several previous reports have described the promiscuous XCI status in long-term cultured female human embryonic stem cells (hESCs), and the majority of them exhibit non-random XCI. However, w...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2016-07-01
|
Series: | Stem Cell Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506116300496 |
id |
doaj-f101b803f0d14d1abb39aae00a4ecd28 |
---|---|
record_format |
Article |
spelling |
doaj-f101b803f0d14d1abb39aae00a4ecd282020-11-24T21:29:57ZengElsevierStem Cell Research1873-50611876-77532016-07-01171849210.1016/j.scr.2016.05.011The dynamic changes of X chromosome inactivation during early culture of human embryonic stem cellsPingyuan Xie0Qi Ouyang1Lizhi Leng2Liang Hu3Dehua Cheng4Yueqiu Tan5Guangxiu Lu6Ge Lin7Institute of Reproductive & Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha 410078, ChinaInstitute of Reproductive & Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha 410078, ChinaInstitute of Reproductive & Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha 410078, ChinaInstitute of Reproductive & Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha 410078, ChinaInstitute of Reproductive & Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha 410078, ChinaInstitute of Reproductive & Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha 410078, ChinaNational Engineering and Research Center of Human Stem Cell, Changsha 410078, ChinaInstitute of Reproductive & Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha 410078, ChinaX chromosome inactivation (XCI) is required for dosage compensation of X-linked genes in human female cells. Several previous reports have described the promiscuous XCI status in long-term cultured female human embryonic stem cells (hESCs), and the majority of them exhibit non-random XCI. However, when and how such female hESCs acquire the aberrant XCI states during culture is unknown. Herein, through comparing the XCI states in 18 paired hES cell lines throughout early culture, we revealed a uniform dynamic change during this culture period under a widely used culture condition. The female initial hESCs (ihESCs, P4-P9) expressed XIST RNA, H3K27me3 punctate enrichment and displayed random XCI pattern. By further culturing, the female early hESCs (ehESCs, P20–P30) lost the expression of XIST RNA, H3K27me3 punctate enrichment and exhibited a completely skewed XCI pattern. Importantly, a subset of X-linked genes was up-regulated in ehESCs, including some cancer-related genes. At last, we found 5% physiological oxygen was beneficial for the expression of XIST and H3K27me3 punctate enrichment, but not for the XCI pattern. We conclude that the XCI dynamic change is a frequent epigenetic instability event during early culture, which is accompanied by the up-regulation of some X-linked genes. Furthermore, we emphasize that physiological oxygen is beneficial for XCI fidelity.http://www.sciencedirect.com/science/article/pii/S1873506116300496Human embryonic stem cellsX chromosome inactivationEpigeneticsGene expression regulationCulture alteration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pingyuan Xie Qi Ouyang Lizhi Leng Liang Hu Dehua Cheng Yueqiu Tan Guangxiu Lu Ge Lin |
spellingShingle |
Pingyuan Xie Qi Ouyang Lizhi Leng Liang Hu Dehua Cheng Yueqiu Tan Guangxiu Lu Ge Lin The dynamic changes of X chromosome inactivation during early culture of human embryonic stem cells Stem Cell Research Human embryonic stem cells X chromosome inactivation Epigenetics Gene expression regulation Culture alteration |
author_facet |
Pingyuan Xie Qi Ouyang Lizhi Leng Liang Hu Dehua Cheng Yueqiu Tan Guangxiu Lu Ge Lin |
author_sort |
Pingyuan Xie |
title |
The dynamic changes of X chromosome inactivation during early culture of human embryonic stem cells |
title_short |
The dynamic changes of X chromosome inactivation during early culture of human embryonic stem cells |
title_full |
The dynamic changes of X chromosome inactivation during early culture of human embryonic stem cells |
title_fullStr |
The dynamic changes of X chromosome inactivation during early culture of human embryonic stem cells |
title_full_unstemmed |
The dynamic changes of X chromosome inactivation during early culture of human embryonic stem cells |
title_sort |
dynamic changes of x chromosome inactivation during early culture of human embryonic stem cells |
publisher |
Elsevier |
series |
Stem Cell Research |
issn |
1873-5061 1876-7753 |
publishDate |
2016-07-01 |
description |
X chromosome inactivation (XCI) is required for dosage compensation of X-linked genes in human female cells. Several previous reports have described the promiscuous XCI status in long-term cultured female human embryonic stem cells (hESCs), and the majority of them exhibit non-random XCI. However, when and how such female hESCs acquire the aberrant XCI states during culture is unknown. Herein, through comparing the XCI states in 18 paired hES cell lines throughout early culture, we revealed a uniform dynamic change during this culture period under a widely used culture condition. The female initial hESCs (ihESCs, P4-P9) expressed XIST RNA, H3K27me3 punctate enrichment and displayed random XCI pattern. By further culturing, the female early hESCs (ehESCs, P20–P30) lost the expression of XIST RNA, H3K27me3 punctate enrichment and exhibited a completely skewed XCI pattern. Importantly, a subset of X-linked genes was up-regulated in ehESCs, including some cancer-related genes. At last, we found 5% physiological oxygen was beneficial for the expression of XIST and H3K27me3 punctate enrichment, but not for the XCI pattern. We conclude that the XCI dynamic change is a frequent epigenetic instability event during early culture, which is accompanied by the up-regulation of some X-linked genes. Furthermore, we emphasize that physiological oxygen is beneficial for XCI fidelity. |
topic |
Human embryonic stem cells X chromosome inactivation Epigenetics Gene expression regulation Culture alteration |
url |
http://www.sciencedirect.com/science/article/pii/S1873506116300496 |
work_keys_str_mv |
AT pingyuanxie thedynamicchangesofxchromosomeinactivationduringearlycultureofhumanembryonicstemcells AT qiouyang thedynamicchangesofxchromosomeinactivationduringearlycultureofhumanembryonicstemcells AT lizhileng thedynamicchangesofxchromosomeinactivationduringearlycultureofhumanembryonicstemcells AT lianghu thedynamicchangesofxchromosomeinactivationduringearlycultureofhumanembryonicstemcells AT dehuacheng thedynamicchangesofxchromosomeinactivationduringearlycultureofhumanembryonicstemcells AT yueqiutan thedynamicchangesofxchromosomeinactivationduringearlycultureofhumanembryonicstemcells AT guangxiulu thedynamicchangesofxchromosomeinactivationduringearlycultureofhumanembryonicstemcells AT gelin thedynamicchangesofxchromosomeinactivationduringearlycultureofhumanembryonicstemcells AT pingyuanxie dynamicchangesofxchromosomeinactivationduringearlycultureofhumanembryonicstemcells AT qiouyang dynamicchangesofxchromosomeinactivationduringearlycultureofhumanembryonicstemcells AT lizhileng dynamicchangesofxchromosomeinactivationduringearlycultureofhumanembryonicstemcells AT lianghu dynamicchangesofxchromosomeinactivationduringearlycultureofhumanembryonicstemcells AT dehuacheng dynamicchangesofxchromosomeinactivationduringearlycultureofhumanembryonicstemcells AT yueqiutan dynamicchangesofxchromosomeinactivationduringearlycultureofhumanembryonicstemcells AT guangxiulu dynamicchangesofxchromosomeinactivationduringearlycultureofhumanembryonicstemcells AT gelin dynamicchangesofxchromosomeinactivationduringearlycultureofhumanembryonicstemcells |
_version_ |
1725964853677391872 |