Protective Effects of Liu Wei Di Huang Wan on the Liver, Orbitofrontal Cortex Nissl Bodies, and Neurites in MSG+PH-Induced Liver Regeneration Rat Model
Introduction. To examine the protective effects of Liu Wei Di Huang Wan formula (LWDH) on liver and orbitofrontal cortex (OFC) injuries in monosodium glutamate (MSG) and partial hepatectomy (PH) rat model. Methods. Neonatal Wistar rats were given MSG or saline on postnatal days 2, 4, 6, 8, and 10. T...
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doaj-f10549b6a2d745eba4d046596b25189d2020-11-25T01:44:38ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882018-01-01201810.1155/2018/90901289090128Protective Effects of Liu Wei Di Huang Wan on the Liver, Orbitofrontal Cortex Nissl Bodies, and Neurites in MSG+PH-Induced Liver Regeneration Rat ModelBin-Bin Zhao0Qing-hua Long1Chao-yang Wang2Lin-lin Chen3Guang-jing Xie4Wen-ji Bo5Bo Xu6Ze-fei Li7Han-Min Li8Ping Wang9Hubei University of Traditional Chinese Medicine, Wuhan 430065, Hubei Province, ChinaHubei University of Traditional Chinese Medicine, Wuhan 430065, Hubei Province, ChinaHubei University of Traditional Chinese Medicine, Wuhan 430065, Hubei Province, ChinaHubei University of Traditional Chinese Medicine, Wuhan 430065, Hubei Province, ChinaHubei University of Traditional Chinese Medicine, Wuhan 430065, Hubei Province, ChinaHubei University of Traditional Chinese Medicine, Wuhan 430065, Hubei Province, ChinaHubei University of Traditional Chinese Medicine, Wuhan 430065, Hubei Province, ChinaHubei University of Traditional Chinese Medicine, Wuhan 430065, Hubei Province, ChinaHubei Provincial Hospital of Traditional Chinese Medicine (Affiliated Hospital to Hubei University of Chinese Medicine, Hubei Institute of Traditional Chinese Medicine), Wuhan 430065, Hubei Province, ChinaHubei University of Traditional Chinese Medicine, Wuhan 430065, Hubei Province, ChinaIntroduction. To examine the protective effects of Liu Wei Di Huang Wan formula (LWDH) on liver and orbitofrontal cortex (OFC) injuries in monosodium glutamate (MSG) and partial hepatectomy (PH) rat model. Methods. Neonatal Wistar rats were given MSG or saline on postnatal days 2, 4, 6, 8, and 10. The rats were caged into five groups and treated accordingly at six weeks old as follows: Saline group, Saline+PH group, MSG group, MSG+PH group, and LWDH group (MSG+PH+LWDH). The PH was performed during week 8 by excision of the left and median hepatic lobes (occupying about 68% of whole liver).On day 8 after the PH, the rats were subjected to an inner OFT before being sacrificed. The liver and OFC were stained using H&E, ORO, or Nissl staining. The expression of neurotrophic factors (β-NGF, BDNF) was examined in the OFC lysates by ELISA. Serum levels of cytokines (IL-1β, VEGF) were examined using the Bio-Plex suspension array. Results. LWDH increased the total distance traveled by the animals (p<0.05), and LWDH improved the integrity of the Nissl bodies in the OFC (mean area of the Nissl bodies, p<0.01; mean diameter, p<0.05; mean density, p<0.05; and IOD, p<0.01). There were less white area in the liver (p>0.05) and decreased hepatic steatosis (p<0.01) in LWDH group. LWDH administration decreased the expression of serum levels of IL-1β (p>0.05), while it increased VEGF (p>0.05) expression. LWDH administration increased the expression of BDNF (p>0.05) and β-NGF (p>0.05) in the OFC, all as compared to the MSG+PH group. Conclusion. LWDH partly protected the animals from depressive-like behaviors in the MSG+PH-induced liver regeneration neonatal rat model. LWDH alleviated hepatic injury and steatosis and, furthermore, protected the Nissl body integrity and the growth of neurites.http://dx.doi.org/10.1155/2018/9090128 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bin-Bin Zhao Qing-hua Long Chao-yang Wang Lin-lin Chen Guang-jing Xie Wen-ji Bo Bo Xu Ze-fei Li Han-Min Li Ping Wang |
spellingShingle |
Bin-Bin Zhao Qing-hua Long Chao-yang Wang Lin-lin Chen Guang-jing Xie Wen-ji Bo Bo Xu Ze-fei Li Han-Min Li Ping Wang Protective Effects of Liu Wei Di Huang Wan on the Liver, Orbitofrontal Cortex Nissl Bodies, and Neurites in MSG+PH-Induced Liver Regeneration Rat Model Evidence-Based Complementary and Alternative Medicine |
author_facet |
Bin-Bin Zhao Qing-hua Long Chao-yang Wang Lin-lin Chen Guang-jing Xie Wen-ji Bo Bo Xu Ze-fei Li Han-Min Li Ping Wang |
author_sort |
Bin-Bin Zhao |
title |
Protective Effects of Liu Wei Di Huang Wan on the Liver, Orbitofrontal Cortex Nissl Bodies, and Neurites in MSG+PH-Induced Liver Regeneration Rat Model |
title_short |
Protective Effects of Liu Wei Di Huang Wan on the Liver, Orbitofrontal Cortex Nissl Bodies, and Neurites in MSG+PH-Induced Liver Regeneration Rat Model |
title_full |
Protective Effects of Liu Wei Di Huang Wan on the Liver, Orbitofrontal Cortex Nissl Bodies, and Neurites in MSG+PH-Induced Liver Regeneration Rat Model |
title_fullStr |
Protective Effects of Liu Wei Di Huang Wan on the Liver, Orbitofrontal Cortex Nissl Bodies, and Neurites in MSG+PH-Induced Liver Regeneration Rat Model |
title_full_unstemmed |
Protective Effects of Liu Wei Di Huang Wan on the Liver, Orbitofrontal Cortex Nissl Bodies, and Neurites in MSG+PH-Induced Liver Regeneration Rat Model |
title_sort |
protective effects of liu wei di huang wan on the liver, orbitofrontal cortex nissl bodies, and neurites in msg+ph-induced liver regeneration rat model |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2018-01-01 |
description |
Introduction. To examine the protective effects of Liu Wei Di Huang Wan formula (LWDH) on liver and orbitofrontal cortex (OFC) injuries in monosodium glutamate (MSG) and partial hepatectomy (PH) rat model. Methods. Neonatal Wistar rats were given MSG or saline on postnatal days 2, 4, 6, 8, and 10. The rats were caged into five groups and treated accordingly at six weeks old as follows: Saline group, Saline+PH group, MSG group, MSG+PH group, and LWDH group (MSG+PH+LWDH). The PH was performed during week 8 by excision of the left and median hepatic lobes (occupying about 68% of whole liver).On day 8 after the PH, the rats were subjected to an inner OFT before being sacrificed. The liver and OFC were stained using H&E, ORO, or Nissl staining. The expression of neurotrophic factors (β-NGF, BDNF) was examined in the OFC lysates by ELISA. Serum levels of cytokines (IL-1β, VEGF) were examined using the Bio-Plex suspension array. Results. LWDH increased the total distance traveled by the animals (p<0.05), and LWDH improved the integrity of the Nissl bodies in the OFC (mean area of the Nissl bodies, p<0.01; mean diameter, p<0.05; mean density, p<0.05; and IOD, p<0.01). There were less white area in the liver (p>0.05) and decreased hepatic steatosis (p<0.01) in LWDH group. LWDH administration decreased the expression of serum levels of IL-1β (p>0.05), while it increased VEGF (p>0.05) expression. LWDH administration increased the expression of BDNF (p>0.05) and β-NGF (p>0.05) in the OFC, all as compared to the MSG+PH group. Conclusion. LWDH partly protected the animals from depressive-like behaviors in the MSG+PH-induced liver regeneration neonatal rat model. LWDH alleviated hepatic injury and steatosis and, furthermore, protected the Nissl body integrity and the growth of neurites. |
url |
http://dx.doi.org/10.1155/2018/9090128 |
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