Potency of an Inactivated Influenza Vaccine against a Challenge with A/Swine/Missouri/A01727926/2015 (H4N6) in Mice for Pandemic Preparedness
H4 influenza viruses have been isolated from birds across the world. In recent years, an H4 influenza virus infection has been confirmed in pigs. Pigs play an important role in the transmission of influenza viruses to human hosts. Therefore, it is important to develop a new vaccine in the case of an...
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doaj-f121f12427c843b8b5af247e02942f3b2020-12-17T00:01:38ZengMDPI AGVaccines2076-393X2020-12-01876876810.3390/vaccines8040768Potency of an Inactivated Influenza Vaccine against a Challenge with A/Swine/Missouri/A01727926/2015 (H4N6) in Mice for Pandemic PreparednessHirotaka Hayashi0Norikazu Isoda1Enkhbold Bazarragchaa2Naoki Nomura3Keita Matsuno4Masatoshi Okamatsu5Hiroshi Kida6Yoshihiro Sakoda7Laboratory of Microbiology, Faculty of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-Ku, Sapporo 060-0818, JapanLaboratory of Microbiology, Faculty of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-Ku, Sapporo 060-0818, JapanLaboratory of Microbiology, Faculty of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-Ku, Sapporo 060-0818, JapanLaboratory of Biologics Development, Research Center for Zoonosis Control, Hokkaido University, Kita 20 Nishi 10, Kita-Ku, Sapporo 001-0020, JapanLaboratory of Microbiology, Faculty of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-Ku, Sapporo 060-0818, JapanLaboratory of Microbiology, Faculty of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-Ku, Sapporo 060-0818, JapanResearch Center for Zoonosis Control, Hokkaido University, Kita 20 Nishi 10, Kita-Ku, Sapporo 001-0020, JapanLaboratory of Microbiology, Faculty of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-Ku, Sapporo 060-0818, JapanH4 influenza viruses have been isolated from birds across the world. In recent years, an H4 influenza virus infection has been confirmed in pigs. Pigs play an important role in the transmission of influenza viruses to human hosts. Therefore, it is important to develop a new vaccine in the case of an H4 influenza virus infection in humans, considering that this virus has a different antigenicity from seasonal human influenza viruses. In this study, after selecting vaccine candidate strains based on their antigenic relation to one of the pig isolates, A/swine/Missouri/A01727926/2015 (H4N6) (MO/15), an inactivated whole-particle vaccine was prepared from A/swan/Hokkaido/481102/2017 (H4N6). This vaccine showed high immunogenicity in mice, and the antibody induced by the vaccine showed high cross-reactivity to the MO/15 virus. This vaccine induced sufficient neutralizing antibodies and mitigated the effects of an MO/15 infection in a mouse model. This study is the first to suggest that an inactivated whole-particle vaccine prepared from an influenza virus isolated from wild birds is an effective countermeasure in case of a future influenza pandemic caused by the H4 influenza virus.https://www.mdpi.com/2076-393X/8/4/768antigenicityH4 influenzaimmunogenicitypandemicvaccine |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hirotaka Hayashi Norikazu Isoda Enkhbold Bazarragchaa Naoki Nomura Keita Matsuno Masatoshi Okamatsu Hiroshi Kida Yoshihiro Sakoda |
spellingShingle |
Hirotaka Hayashi Norikazu Isoda Enkhbold Bazarragchaa Naoki Nomura Keita Matsuno Masatoshi Okamatsu Hiroshi Kida Yoshihiro Sakoda Potency of an Inactivated Influenza Vaccine against a Challenge with A/Swine/Missouri/A01727926/2015 (H4N6) in Mice for Pandemic Preparedness Vaccines antigenicity H4 influenza immunogenicity pandemic vaccine |
author_facet |
Hirotaka Hayashi Norikazu Isoda Enkhbold Bazarragchaa Naoki Nomura Keita Matsuno Masatoshi Okamatsu Hiroshi Kida Yoshihiro Sakoda |
author_sort |
Hirotaka Hayashi |
title |
Potency of an Inactivated Influenza Vaccine against a Challenge with A/Swine/Missouri/A01727926/2015 (H4N6) in Mice for Pandemic Preparedness |
title_short |
Potency of an Inactivated Influenza Vaccine against a Challenge with A/Swine/Missouri/A01727926/2015 (H4N6) in Mice for Pandemic Preparedness |
title_full |
Potency of an Inactivated Influenza Vaccine against a Challenge with A/Swine/Missouri/A01727926/2015 (H4N6) in Mice for Pandemic Preparedness |
title_fullStr |
Potency of an Inactivated Influenza Vaccine against a Challenge with A/Swine/Missouri/A01727926/2015 (H4N6) in Mice for Pandemic Preparedness |
title_full_unstemmed |
Potency of an Inactivated Influenza Vaccine against a Challenge with A/Swine/Missouri/A01727926/2015 (H4N6) in Mice for Pandemic Preparedness |
title_sort |
potency of an inactivated influenza vaccine against a challenge with a/swine/missouri/a01727926/2015 (h4n6) in mice for pandemic preparedness |
publisher |
MDPI AG |
series |
Vaccines |
issn |
2076-393X |
publishDate |
2020-12-01 |
description |
H4 influenza viruses have been isolated from birds across the world. In recent years, an H4 influenza virus infection has been confirmed in pigs. Pigs play an important role in the transmission of influenza viruses to human hosts. Therefore, it is important to develop a new vaccine in the case of an H4 influenza virus infection in humans, considering that this virus has a different antigenicity from seasonal human influenza viruses. In this study, after selecting vaccine candidate strains based on their antigenic relation to one of the pig isolates, A/swine/Missouri/A01727926/2015 (H4N6) (MO/15), an inactivated whole-particle vaccine was prepared from A/swan/Hokkaido/481102/2017 (H4N6). This vaccine showed high immunogenicity in mice, and the antibody induced by the vaccine showed high cross-reactivity to the MO/15 virus. This vaccine induced sufficient neutralizing antibodies and mitigated the effects of an MO/15 infection in a mouse model. This study is the first to suggest that an inactivated whole-particle vaccine prepared from an influenza virus isolated from wild birds is an effective countermeasure in case of a future influenza pandemic caused by the H4 influenza virus. |
topic |
antigenicity H4 influenza immunogenicity pandemic vaccine |
url |
https://www.mdpi.com/2076-393X/8/4/768 |
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