Molecular and Serological Footprints of <i>Mycobacterium avium</i> Subspecies Infections in Zoo Animals
Background: Mycobacteria of the <i>Mycobacterium avium</i> complex (MAC) pose a significant risk to zoological collections. <i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i> (MAP) is a member of MAC and the causative agent of Johne’s disease. Despite m...
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doaj-f152837361cf40f1aa57a8b05b4d83be2021-04-02T15:38:33ZengMDPI AGVeterinary Sciences2306-73812020-08-01711711710.3390/vetsci7030117Molecular and Serological Footprints of <i>Mycobacterium avium</i> Subspecies Infections in Zoo AnimalsMarco Roller0Sören Hansen1Susanne Böhlken-Fascher2Tobias Knauf-Witzens3Claus-Peter Czerny4Ralph Goethe5Ahmed Abd El Wahed6Wilhelma Zoological-Botanical Gardens Stuttgart, Wilhelma 13, D-70376 Stuttgart, GermanyDepartment of Animal Sciences, Division of Microbiology and Animal Hygiene, Faculty of Agricultural Science, Georg-August-University, Burckhardtweg 2, D-37077 Göttingen, GermanyDepartment of Animal Sciences, Division of Microbiology and Animal Hygiene, Faculty of Agricultural Science, Georg-August-University, Burckhardtweg 2, D-37077 Göttingen, GermanyWilhelma Zoological-Botanical Gardens Stuttgart, Wilhelma 13, D-70376 Stuttgart, GermanyDepartment of Animal Sciences, Division of Microbiology and Animal Hygiene, Faculty of Agricultural Science, Georg-August-University, Burckhardtweg 2, D-37077 Göttingen, GermanyInstitute for Microbiology, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, D-30173 Hannover, GermanyDepartment of Animal Sciences, Division of Microbiology and Animal Hygiene, Faculty of Agricultural Science, Georg-August-University, Burckhardtweg 2, D-37077 Göttingen, GermanyBackground: Mycobacteria of the <i>Mycobacterium avium</i> complex (MAC) pose a significant risk to zoological collections. <i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i> (MAP) is a member of MAC and the causative agent of Johne’s disease. Despite many reports in animals kept in zoological gardens, systemic surveillance has rarely been reported. Methods: In this study, archived serum samples collected from animal species at the Wilhelma Zoological and Botanical Gardens in Stuttgart, Germany, were screened for the presence of antibodies against MAC and MAP. In addition, molecular investigations were performed on necropsy, fecal, and environmental samples. Results: In total, 30/381 serum samples of various mammalian species were positive for MAC antibodies in ELISA, while one sample of a reticulated giraffe (<i>Giraffa camelopardalis reticulata</i>) was positive in MAP-specific ELISA. Samples from many species were positive in pan-<i>Mycobacterium</i> real-time PCR (40/43 fecal samples, 27/43 environmental samples, and 31/90 necropsy samples). Surprisingly, no sample was positive in the MAP-specific molecular assays. However, two environmental samples from primate enclosures were positive in <i>Mycobacterium avium</i> subspecies <i>hominissuis</i> (MAH)-specific real-time PCR. Conclusions: The results reveal serological indications of MAC infections in the zoological collection. However, the presence of a MAP-contaminated environment by a high-shedding individual animal or MAP-infected population is unlikely.https://www.mdpi.com/2306-7381/7/3/117<i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i>Johne’s diseaseparatuberculosis<i>Mycobacterium avium</i> complexzoo animalsserological assays |
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language |
English |
format |
Article |
sources |
DOAJ |
author |
Marco Roller Sören Hansen Susanne Böhlken-Fascher Tobias Knauf-Witzens Claus-Peter Czerny Ralph Goethe Ahmed Abd El Wahed |
spellingShingle |
Marco Roller Sören Hansen Susanne Böhlken-Fascher Tobias Knauf-Witzens Claus-Peter Czerny Ralph Goethe Ahmed Abd El Wahed Molecular and Serological Footprints of <i>Mycobacterium avium</i> Subspecies Infections in Zoo Animals Veterinary Sciences <i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i> Johne’s disease paratuberculosis <i>Mycobacterium avium</i> complex zoo animals serological assays |
author_facet |
Marco Roller Sören Hansen Susanne Böhlken-Fascher Tobias Knauf-Witzens Claus-Peter Czerny Ralph Goethe Ahmed Abd El Wahed |
author_sort |
Marco Roller |
title |
Molecular and Serological Footprints of <i>Mycobacterium avium</i> Subspecies Infections in Zoo Animals |
title_short |
Molecular and Serological Footprints of <i>Mycobacterium avium</i> Subspecies Infections in Zoo Animals |
title_full |
Molecular and Serological Footprints of <i>Mycobacterium avium</i> Subspecies Infections in Zoo Animals |
title_fullStr |
Molecular and Serological Footprints of <i>Mycobacterium avium</i> Subspecies Infections in Zoo Animals |
title_full_unstemmed |
Molecular and Serological Footprints of <i>Mycobacterium avium</i> Subspecies Infections in Zoo Animals |
title_sort |
molecular and serological footprints of <i>mycobacterium avium</i> subspecies infections in zoo animals |
publisher |
MDPI AG |
series |
Veterinary Sciences |
issn |
2306-7381 |
publishDate |
2020-08-01 |
description |
Background: Mycobacteria of the <i>Mycobacterium avium</i> complex (MAC) pose a significant risk to zoological collections. <i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i> (MAP) is a member of MAC and the causative agent of Johne’s disease. Despite many reports in animals kept in zoological gardens, systemic surveillance has rarely been reported. Methods: In this study, archived serum samples collected from animal species at the Wilhelma Zoological and Botanical Gardens in Stuttgart, Germany, were screened for the presence of antibodies against MAC and MAP. In addition, molecular investigations were performed on necropsy, fecal, and environmental samples. Results: In total, 30/381 serum samples of various mammalian species were positive for MAC antibodies in ELISA, while one sample of a reticulated giraffe (<i>Giraffa camelopardalis reticulata</i>) was positive in MAP-specific ELISA. Samples from many species were positive in pan-<i>Mycobacterium</i> real-time PCR (40/43 fecal samples, 27/43 environmental samples, and 31/90 necropsy samples). Surprisingly, no sample was positive in the MAP-specific molecular assays. However, two environmental samples from primate enclosures were positive in <i>Mycobacterium avium</i> subspecies <i>hominissuis</i> (MAH)-specific real-time PCR. Conclusions: The results reveal serological indications of MAC infections in the zoological collection. However, the presence of a MAP-contaminated environment by a high-shedding individual animal or MAP-infected population is unlikely. |
topic |
<i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i> Johne’s disease paratuberculosis <i>Mycobacterium avium</i> complex zoo animals serological assays |
url |
https://www.mdpi.com/2306-7381/7/3/117 |
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