Evaluation of the Liaison® C. difficile GDH System
<strong>Objectives</strong>. The aim of our study was to evaluate the LIAISON® system for the detection of GDH of C. difficile by comparing the results with those obtained with the our routine EIA test, from both of unselected samples and selected samples according to the Bristol scale f...
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doaj-f1659a76887a4acbb2b7771248426a6a2020-11-25T03:58:34ZengPAGEPress PublicationsMicrobiologia Medica 2280-64232013-08-0128210.4081/mm.2013.22551514Evaluation of the Liaison® C. difficile GDH SystemMassimo De Paschale0Maria Teresa Manco1Emanuela Vasconi2Alessia Paganini3Monica Barzani4Carlo Agrappi5Paola Mirri6Pierangelo Clerici7U.O.C. Microbiologia, A.O. Ospedale Civile di Legnano, Legnano (Mi)U.O.C. Microbiologia, A.O. Ospedale Civile di Legnano, Legnano (Mi)U.O.C. Microbiologia, A.O. Ospedale Civile di Legnano, Legnano (Mi)U.O.C. Microbiologia, A.O. Ospedale Civile di Legnano, Legnano (Mi)U.O.C. Microbiologia, A.O. Ospedale Civile di Legnano, Legnano (Mi)U.O.C. Microbiologia, A.O. Ospedale Civile di Legnano, Legnano (Mi)U.O.C. Microbiologia, A.O. Ospedale Civile di Legnano, Legnano (Mi)U.O.C. Microbiologia, A.O. Ospedale Civile di Legnano, Legnano (Mi)<strong>Objectives</strong>. The aim of our study was to evaluate the LIAISON® system for the detection of GDH of C. difficile by comparing the results with those obtained with the our routine EIA test, from both of unselected samples and selected samples according to the Bristol scale for stool consistency. <strong>Study Design</strong>. We examined 114 unselected stool samples (Group 1) and 104 selected samples according to the Bristol scale (type 4-7: Group 2) for the detection of GDH with enzyme immunoassay (EIA) and chemiluminescence LIAISON® system. In case of positivity for one or both tests toxins A and B were searched, with EIA, and, in case of negativity of the toxins, the test of gene amplification for the search of the locus of pathogenicity for toxins A and B was performed. <strong>Results</strong>. In Group 1, sixteen samples were positive for both tests for GDH, 93 negative for both tests, and five discordant (three EIA positive and LIAISON® negative; two EIA negative and LIAISON® positive): total concordance between the two methods: 95.6%. Of the five discordant samples, all were toxin A & B negative, of these only one was positive to molecular biology. In Group 2, fifteen samples were positive for both tests for GDH, 89 negative for both tests and no discordant: total concordance between the two methods: 100%. <strong>Conclusions</strong>. Our data indicate a perfect correlation between LIAISON® and EIA, when samples are chosen according to stool consistency at least grade 4 of the Bristol scale.The possibility to use an automatic and fast system, like LIAISON®, also allows an optimization of workflows.http://www.pagepressjournals.org/index.php/mm/article/view/2255C. difficile, GDH, Chemiluminescence, Bristol scale |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Massimo De Paschale Maria Teresa Manco Emanuela Vasconi Alessia Paganini Monica Barzani Carlo Agrappi Paola Mirri Pierangelo Clerici |
spellingShingle |
Massimo De Paschale Maria Teresa Manco Emanuela Vasconi Alessia Paganini Monica Barzani Carlo Agrappi Paola Mirri Pierangelo Clerici Evaluation of the Liaison® C. difficile GDH System Microbiologia Medica C. difficile, GDH, Chemiluminescence, Bristol scale |
author_facet |
Massimo De Paschale Maria Teresa Manco Emanuela Vasconi Alessia Paganini Monica Barzani Carlo Agrappi Paola Mirri Pierangelo Clerici |
author_sort |
Massimo De Paschale |
title |
Evaluation of the Liaison® C. difficile GDH System |
title_short |
Evaluation of the Liaison® C. difficile GDH System |
title_full |
Evaluation of the Liaison® C. difficile GDH System |
title_fullStr |
Evaluation of the Liaison® C. difficile GDH System |
title_full_unstemmed |
Evaluation of the Liaison® C. difficile GDH System |
title_sort |
evaluation of the liaison® c. difficile gdh system |
publisher |
PAGEPress Publications |
series |
Microbiologia Medica |
issn |
2280-6423 |
publishDate |
2013-08-01 |
description |
<strong>Objectives</strong>. The aim of our study was to evaluate the LIAISON® system for the detection of GDH of C. difficile by comparing the results with those obtained with the our routine EIA test, from both of unselected samples and selected samples according to the Bristol scale for stool consistency. <strong>Study Design</strong>. We examined 114 unselected stool samples (Group 1) and 104 selected samples according to the Bristol scale (type 4-7: Group 2) for the detection of GDH with enzyme immunoassay (EIA) and chemiluminescence LIAISON® system. In case of positivity for one or both tests toxins A and B were searched, with EIA, and, in case of negativity of the toxins, the test of gene amplification for the search of the locus of pathogenicity for toxins A and B was performed. <strong>Results</strong>. In Group 1, sixteen samples were positive for both tests for GDH, 93 negative for both tests, and five discordant (three EIA positive and LIAISON® negative; two EIA negative and LIAISON® positive): total concordance between the two methods: 95.6%. Of the five discordant samples, all were toxin A & B negative, of these only one was positive to molecular biology. In Group 2, fifteen samples were positive for both tests for GDH, 89 negative for both tests and no discordant: total concordance between the two methods: 100%. <strong>Conclusions</strong>. Our data indicate a perfect correlation between LIAISON® and EIA, when samples are chosen according to stool consistency at least grade 4 of the Bristol scale.The possibility to use an automatic and fast system, like LIAISON®, also allows an optimization of workflows. |
topic |
C. difficile, GDH, Chemiluminescence, Bristol scale |
url |
http://www.pagepressjournals.org/index.php/mm/article/view/2255 |
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