Towards PCB-Based Miniaturized Thermocyclers for DNA Amplification
In recent years, printed circuit board (PCB)-based microfluidics have been explored as a means to achieve standardization, seamless integration, and large-scale manufacturing of microfluidics, thus paving the way for widespread commercialization of developed prototypes. In this work, static micro po...
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doaj-f1a26c9a90c14240a724701fb1e8150b2020-11-25T02:56:04ZengMDPI AGMicromachines2072-666X2020-02-0111325810.3390/mi11030258mi11030258Towards PCB-Based Miniaturized Thermocyclers for DNA AmplificationGeorgia D. Kaprou0Vasileios Papadopoulos1Christos-Moritz Loukas2George Kokkoris3Angeliki Tserepi4Institute of Nanoscience and Nanotechnology, National Center for Scientific Research “Demokritos”, Patr. Gregoriou E’ and 27 Neapoleos str., 15341 Aghia Paraskevi, Attiki, GreeceInstitute of Nanoscience and Nanotechnology, National Center for Scientific Research “Demokritos”, Patr. Gregoriou E’ and 27 Neapoleos str., 15341 Aghia Paraskevi, Attiki, GreeceInstitute of Nanoscience and Nanotechnology, National Center for Scientific Research “Demokritos”, Patr. Gregoriou E’ and 27 Neapoleos str., 15341 Aghia Paraskevi, Attiki, GreeceInstitute of Nanoscience and Nanotechnology, National Center for Scientific Research “Demokritos”, Patr. Gregoriou E’ and 27 Neapoleos str., 15341 Aghia Paraskevi, Attiki, GreeceInstitute of Nanoscience and Nanotechnology, National Center for Scientific Research “Demokritos”, Patr. Gregoriou E’ and 27 Neapoleos str., 15341 Aghia Paraskevi, Attiki, GreeceIn recent years, printed circuit board (PCB)-based microfluidics have been explored as a means to achieve standardization, seamless integration, and large-scale manufacturing of microfluidics, thus paving the way for widespread commercialization of developed prototypes. In this work, static micro polymerase chain reaction (microPCR) devices comprising resistive microheaters integrated on PCBs are introduced as miniaturized thermocyclers for efficient DNA amplification. Their performance is compared to that of conventional thermocyclers, in terms of amplification efficiency, power consumption and duration. Exhibiting similar efficiency to conventional thermocyclers, PCB-based miniaturized thermocycling achieves faster DNA amplification, with significantly smaller power consumption. Simulations guide the design of such devices and propose means for further improvement of their performance.https://www.mdpi.com/2072-666X/11/3/258printed circuit board (pcb)microfluidicsmicro polymerase chain reaction (micropcr)staticmulti-well pcrsimulationgenomic <i>salmonella</i> dna |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Georgia D. Kaprou Vasileios Papadopoulos Christos-Moritz Loukas George Kokkoris Angeliki Tserepi |
spellingShingle |
Georgia D. Kaprou Vasileios Papadopoulos Christos-Moritz Loukas George Kokkoris Angeliki Tserepi Towards PCB-Based Miniaturized Thermocyclers for DNA Amplification Micromachines printed circuit board (pcb) microfluidics micro polymerase chain reaction (micropcr) static multi-well pcr simulation genomic <i>salmonella</i> dna |
author_facet |
Georgia D. Kaprou Vasileios Papadopoulos Christos-Moritz Loukas George Kokkoris Angeliki Tserepi |
author_sort |
Georgia D. Kaprou |
title |
Towards PCB-Based Miniaturized Thermocyclers for DNA Amplification |
title_short |
Towards PCB-Based Miniaturized Thermocyclers for DNA Amplification |
title_full |
Towards PCB-Based Miniaturized Thermocyclers for DNA Amplification |
title_fullStr |
Towards PCB-Based Miniaturized Thermocyclers for DNA Amplification |
title_full_unstemmed |
Towards PCB-Based Miniaturized Thermocyclers for DNA Amplification |
title_sort |
towards pcb-based miniaturized thermocyclers for dna amplification |
publisher |
MDPI AG |
series |
Micromachines |
issn |
2072-666X |
publishDate |
2020-02-01 |
description |
In recent years, printed circuit board (PCB)-based microfluidics have been explored as a means to achieve standardization, seamless integration, and large-scale manufacturing of microfluidics, thus paving the way for widespread commercialization of developed prototypes. In this work, static micro polymerase chain reaction (microPCR) devices comprising resistive microheaters integrated on PCBs are introduced as miniaturized thermocyclers for efficient DNA amplification. Their performance is compared to that of conventional thermocyclers, in terms of amplification efficiency, power consumption and duration. Exhibiting similar efficiency to conventional thermocyclers, PCB-based miniaturized thermocycling achieves faster DNA amplification, with significantly smaller power consumption. Simulations guide the design of such devices and propose means for further improvement of their performance. |
topic |
printed circuit board (pcb) microfluidics micro polymerase chain reaction (micropcr) static multi-well pcr simulation genomic <i>salmonella</i> dna |
url |
https://www.mdpi.com/2072-666X/11/3/258 |
work_keys_str_mv |
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