Population structure and association mapping to detect QTL controlling tomato spotted wilt virus resistance in cultivated peanuts
Tomato spotted wilt (TSW) is a serious virus disease of peanut in the United States. Breeding for TSWV resistance would be facilitated by the implementation of marker-assisted selection in breeding programs; however, genes associated with resistance have not been identified. Association mapping is a...
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2018-10-01
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doaj-f1acc6a0c92342b1bbc96d7a4f4599f42021-02-02T06:07:58ZengKeAi Communications Co., Ltd.Crop Journal2214-51412018-10-0165516526Population structure and association mapping to detect QTL controlling tomato spotted wilt virus resistance in cultivated peanutsJing Li0Yueyi Tang1Alana L. Jacobson2Phat M. Dang3Xiao Li4Ming Li Wang5Austin Hagan6Charles Y. Chen7Department of Crop, Soil and Environmental Sciences, Auburn University, Auburn, AL 36849, USADepartment of Crop, Soil and Environmental Sciences, Auburn University, Auburn, AL 36849, USA; Shandong Peanut Research Institute, Qingdao 266100, ChinaDepartment of Entomology and Plant Pathology, Auburn University, Auburn, AL 36849, USAUSDA-ARS National Peanut Research Laboratory, Dawson, GA 39842, USADepartment of Crop, Soil and Environmental Sciences, Auburn University, Auburn, AL 36849, USAUSDA-ARS Plant Genetic Resources Conservation Unit, Griffin, GA 30223, USADepartment of Entomology and Plant Pathology, Auburn University, Auburn, AL 36849, USADepartment of Crop, Soil and Environmental Sciences, Auburn University, Auburn, AL 36849, USA; Corresponding author.Tomato spotted wilt (TSW) is a serious virus disease of peanut in the United States. Breeding for TSWV resistance would be facilitated by the implementation of marker-assisted selection in breeding programs; however, genes associated with resistance have not been identified. Association mapping is a type of genetic mapping that can exploit relationships between markers and traits in many lineages. The objectives of this study were to examine genetic diversity and population structure in the U.S. peanut mini-core collection using simple sequence repeat (SSR) markers, and to conduct association mapping between SSR markers and TSWV resistance in cultivated peanuts. One hundred and thirty-three SSR markers were used for genotyping 104 accessions. Four subpopulations, generally corresponding to botanical varieties, were classified by population structure analysis. Association mapping analysis indicated that five markers: pPGPseq5D5, GM1135, GM1991, TC23C08, and TC24C06, were consistently associated with TSW resistance by the Q, PCA, Q+K, and PCA+K models. These markers together explained 36.4% of the phenotypic variance. Moreover, pPGPseq5D5 and GM1991 were associated with both visual symptoms of TSWV and ELISA values with a high R2. The potential of these markers for use in a marker-assisted selection program to breed peanut for resistance to TSWV is discussed. Keywords: Association mapping, SSR markers, Tomato spotted wilt virus, Peanutshttp://www.sciencedirect.com/science/article/pii/S2214514118300588 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jing Li Yueyi Tang Alana L. Jacobson Phat M. Dang Xiao Li Ming Li Wang Austin Hagan Charles Y. Chen |
spellingShingle |
Jing Li Yueyi Tang Alana L. Jacobson Phat M. Dang Xiao Li Ming Li Wang Austin Hagan Charles Y. Chen Population structure and association mapping to detect QTL controlling tomato spotted wilt virus resistance in cultivated peanuts Crop Journal |
author_facet |
Jing Li Yueyi Tang Alana L. Jacobson Phat M. Dang Xiao Li Ming Li Wang Austin Hagan Charles Y. Chen |
author_sort |
Jing Li |
title |
Population structure and association mapping to detect QTL controlling tomato spotted wilt virus resistance in cultivated peanuts |
title_short |
Population structure and association mapping to detect QTL controlling tomato spotted wilt virus resistance in cultivated peanuts |
title_full |
Population structure and association mapping to detect QTL controlling tomato spotted wilt virus resistance in cultivated peanuts |
title_fullStr |
Population structure and association mapping to detect QTL controlling tomato spotted wilt virus resistance in cultivated peanuts |
title_full_unstemmed |
Population structure and association mapping to detect QTL controlling tomato spotted wilt virus resistance in cultivated peanuts |
title_sort |
population structure and association mapping to detect qtl controlling tomato spotted wilt virus resistance in cultivated peanuts |
publisher |
KeAi Communications Co., Ltd. |
series |
Crop Journal |
issn |
2214-5141 |
publishDate |
2018-10-01 |
description |
Tomato spotted wilt (TSW) is a serious virus disease of peanut in the United States. Breeding for TSWV resistance would be facilitated by the implementation of marker-assisted selection in breeding programs; however, genes associated with resistance have not been identified. Association mapping is a type of genetic mapping that can exploit relationships between markers and traits in many lineages. The objectives of this study were to examine genetic diversity and population structure in the U.S. peanut mini-core collection using simple sequence repeat (SSR) markers, and to conduct association mapping between SSR markers and TSWV resistance in cultivated peanuts. One hundred and thirty-three SSR markers were used for genotyping 104 accessions. Four subpopulations, generally corresponding to botanical varieties, were classified by population structure analysis. Association mapping analysis indicated that five markers: pPGPseq5D5, GM1135, GM1991, TC23C08, and TC24C06, were consistently associated with TSW resistance by the Q, PCA, Q+K, and PCA+K models. These markers together explained 36.4% of the phenotypic variance. Moreover, pPGPseq5D5 and GM1991 were associated with both visual symptoms of TSWV and ELISA values with a high R2. The potential of these markers for use in a marker-assisted selection program to breed peanut for resistance to TSWV is discussed. Keywords: Association mapping, SSR markers, Tomato spotted wilt virus, Peanuts |
url |
http://www.sciencedirect.com/science/article/pii/S2214514118300588 |
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