THE EFFECT OF ERYTHROPOIETIN SUPPLEMENTATION ON SPERM MOTILITY AND MORPHOLOGY IN WISTAR RAT AFTER LIGATION RELEASE OF THE VAS DEFERENS
Objective: The patency rates after vasectomy reversal ranges from 71-97%, but there is 26-72% possibility of persistent infertility. Dysfunction or obstruction of the epididymis and oxidative stress are thought to be the important cause of male infertility by disrupting spermatozoa maturation proces...
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IKATAN AHLI UROLOGI INDONESIA
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doaj-f1bb5ebdd3ae4f23b7025a90c3bc08e02020-11-25T03:38:37ZengIKATAN AHLI UROLOGI INDONESIAJurnal Urologi Indonesia 0853-442X2355-14022019-07-0126210.32421/juri.v26i2.501501THE EFFECT OF ERYTHROPOIETIN SUPPLEMENTATION ON SPERM MOTILITY AND MORPHOLOGY IN WISTAR RAT AFTER LIGATION RELEASE OF THE VAS DEFERENSAditya Pramanta0Johan Renaldo1Doddy M Soebadi2Department of Urology, Faculty of Medicine/Universitas Airlangga, Soetomo General Hospital, Surabaya.Department of Urology, Faculty of Medicine/Universitas Airlangga, Soetomo General Hospital, Surabaya.Department of Urology, Faculty of Medicine/Universitas Airlangga, Soetomo General Hospital, Surabaya.Objective: The patency rates after vasectomy reversal ranges from 71-97%, but there is 26-72% possibility of persistent infertility. Dysfunction or obstruction of the epididymis and oxidative stress are thought to be the important cause of male infertility by disrupting spermatozoa maturation process, causing poor sperm quality. Human erythropoietin or better known as EPO is a glycoprotein hormone that has been purified since three decades ago. Research on the EPO has evolved and become a major research topic the researchers aimed as a therapeutic agent. The cloning and expression of erythropoietin has developed recombinant erythropoietin as a drug that serves as an anti-oxidant, anti-apoptotic and anti-inflammatory. This study aimed to determine the effect of erythropoietin supplementation on sperm motility and morphology in Wistar rat after the release of the vas deferens’ ligation. Material & Methods: Twenty four male Wistar rats were randomly divided into four groups (6 each). On the vasectomy group, the vas deferens were serially ligated for 7 weeks using a non-absorbable suture. The vasectomy reversal group get the same surgical treatment and after 7 weeks the ligation were released. While as in the erythropoietin group, recombinant eryhtropoietin (1000 IU/kg) was administered intraperitoneally three times for 1 week after releasing the ligation. Normal control animals received no surgical manipulation and followed by sperm retrieval for analysis. Eosin-stained slides were prepared to assess the motility and morphology of sperm cells and observed under a light microscope. Results: Ligation of vas deferens significantly decreased sperm motility and morphology. Releasing the ligation of the vas deferens did not improve the sperm motility and morphology. Supplementing eryhtropoietin 1000 IU/kg 3 times for a week after releasing the ligation did not improve the sperm motility and morphology. Conclusion: Erythropoietin supplementation did not improve the sperm motility and morphology in Wistar rat after releasing the ligation of vas deferens. http://juri.urologi.or.id/juri/article/view/501Vasectomyreversaleryhtropoietinsperm motilitysperm morphology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aditya Pramanta Johan Renaldo Doddy M Soebadi |
spellingShingle |
Aditya Pramanta Johan Renaldo Doddy M Soebadi THE EFFECT OF ERYTHROPOIETIN SUPPLEMENTATION ON SPERM MOTILITY AND MORPHOLOGY IN WISTAR RAT AFTER LIGATION RELEASE OF THE VAS DEFERENS Jurnal Urologi Indonesia Vasectomy reversal eryhtropoietin sperm motility sperm morphology |
author_facet |
Aditya Pramanta Johan Renaldo Doddy M Soebadi |
author_sort |
Aditya Pramanta |
title |
THE EFFECT OF ERYTHROPOIETIN SUPPLEMENTATION ON SPERM MOTILITY AND MORPHOLOGY IN WISTAR RAT AFTER LIGATION RELEASE OF THE VAS DEFERENS |
title_short |
THE EFFECT OF ERYTHROPOIETIN SUPPLEMENTATION ON SPERM MOTILITY AND MORPHOLOGY IN WISTAR RAT AFTER LIGATION RELEASE OF THE VAS DEFERENS |
title_full |
THE EFFECT OF ERYTHROPOIETIN SUPPLEMENTATION ON SPERM MOTILITY AND MORPHOLOGY IN WISTAR RAT AFTER LIGATION RELEASE OF THE VAS DEFERENS |
title_fullStr |
THE EFFECT OF ERYTHROPOIETIN SUPPLEMENTATION ON SPERM MOTILITY AND MORPHOLOGY IN WISTAR RAT AFTER LIGATION RELEASE OF THE VAS DEFERENS |
title_full_unstemmed |
THE EFFECT OF ERYTHROPOIETIN SUPPLEMENTATION ON SPERM MOTILITY AND MORPHOLOGY IN WISTAR RAT AFTER LIGATION RELEASE OF THE VAS DEFERENS |
title_sort |
effect of erythropoietin supplementation on sperm motility and morphology in wistar rat after ligation release of the vas deferens |
publisher |
IKATAN AHLI UROLOGI INDONESIA |
series |
Jurnal Urologi Indonesia |
issn |
0853-442X 2355-1402 |
publishDate |
2019-07-01 |
description |
Objective: The patency rates after vasectomy reversal ranges from 71-97%, but there is 26-72% possibility of persistent infertility. Dysfunction or obstruction of the epididymis and oxidative stress are thought to be the important cause of male infertility by disrupting spermatozoa maturation process, causing poor sperm quality. Human erythropoietin or better known as EPO is a glycoprotein hormone that has been purified since three decades ago. Research on the EPO has evolved and become a major research topic the researchers aimed as a therapeutic agent. The cloning and expression of erythropoietin has developed recombinant erythropoietin as a drug that serves as an anti-oxidant, anti-apoptotic and anti-inflammatory. This study aimed to determine the effect of erythropoietin supplementation on sperm motility and morphology in Wistar rat after the release of the vas deferens’ ligation. Material & Methods: Twenty four male Wistar rats were randomly divided into four groups (6 each). On the vasectomy group, the vas deferens were serially ligated for 7 weeks using a non-absorbable suture. The vasectomy reversal group get the same surgical treatment and after 7 weeks the ligation were released. While as in the erythropoietin group, recombinant eryhtropoietin (1000 IU/kg) was administered intraperitoneally three times for 1 week after releasing the ligation. Normal control animals received no surgical manipulation and followed by sperm retrieval for analysis. Eosin-stained slides were prepared to assess the motility and morphology of sperm cells and observed under a light microscope. Results: Ligation of vas deferens significantly decreased sperm motility and morphology. Releasing the ligation of the vas deferens did not improve the sperm motility and morphology. Supplementing eryhtropoietin 1000 IU/kg 3 times for a week after releasing the ligation did not improve the sperm motility and morphology. Conclusion: Erythropoietin supplementation did not improve the sperm motility and morphology in Wistar rat after releasing the ligation of vas deferens.
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topic |
Vasectomy reversal eryhtropoietin sperm motility sperm morphology |
url |
http://juri.urologi.or.id/juri/article/view/501 |
work_keys_str_mv |
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