Association Study of Estrogen Receptor Alpha Gene Polymorphisms with Spontaneous Abortion: Is This a Possible Reason for Unexplained Spontaneous Abortion?
Estrogen plays a crucial role in fetal and placental development through estrogen receptors. Association of estrogen receptor alpha gene (ESR1) polymorphisms with spontaneous abortion has been shown in some studies. Our main goal was to study the potential association of spontaneous abortion with th...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2013-01-01
|
Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2013/256470 |
id |
doaj-f1bf8d06573d43b899c6f3ccbf0aaeb1 |
---|---|
record_format |
Article |
spelling |
doaj-f1bf8d06573d43b899c6f3ccbf0aaeb12020-11-25T01:20:30ZengHindawi LimitedBioMed Research International2314-61332314-61412013-01-01201310.1155/2013/256470256470Association Study of Estrogen Receptor Alpha Gene Polymorphisms with Spontaneous Abortion: Is This a Possible Reason for Unexplained Spontaneous Abortion?Negin Anousha0Arash Hossein-Nezhad1Firouzeh Biramijamal2Ali Rahmani3Zhila Maghbooli4Elahe Aghababaei5Shahram Nemati6Department of Genetics, Ahar Branch, Islamic Azad University, Ahar 54511, IranDepartment of Medicine, Section of Endocrinology, Nutrition, and Diabetes, Vitamin D, Skin and Bone Research Laboratory, Boston University Medical Center, Boston, MA 02118, USADepartment of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran 14178, IranDepartment of Genetics, Ahar Branch, Islamic Azad University, Ahar 54511, IranEndocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran 14114, IranEndocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran 14114, IranEndocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran 14114, IranEstrogen plays a crucial role in fetal and placental development through estrogen receptors. Association of estrogen receptor alpha gene (ESR1) polymorphisms with spontaneous abortion has been shown in some studies. Our main goal was to study the potential association of spontaneous abortion with the ESR1 gene variations (PvuII and XbaI) in fetal tissue. Totally, 161 samples were recruited including 80 samples of formalin-fixed paraffin-embedded fetal tissue from spontaneous abortion and 81 samples of normal term placental tissue. The restriction fragment length polymorphism (RFLP) method was performed for genotyping the rs2234693 (A/G XbaI) and rs9340799 (T/C PvuII) single nucleotide polymorphisms located in intron 1 of ESR1. The results have been confirmed by DNA sequencing analysis. The different genotypes distribution was detected in two study groups. Haplotype analysis indicated that ppxx is protective genotype against spontaneous abortion (P = 0.01). In conclusion, the potential role of ESR1 genetic variation in spontaneous abortion might be valuable in high-risk subjects, and that needs to be confirmed with future studies.http://dx.doi.org/10.1155/2013/256470 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Negin Anousha Arash Hossein-Nezhad Firouzeh Biramijamal Ali Rahmani Zhila Maghbooli Elahe Aghababaei Shahram Nemati |
spellingShingle |
Negin Anousha Arash Hossein-Nezhad Firouzeh Biramijamal Ali Rahmani Zhila Maghbooli Elahe Aghababaei Shahram Nemati Association Study of Estrogen Receptor Alpha Gene Polymorphisms with Spontaneous Abortion: Is This a Possible Reason for Unexplained Spontaneous Abortion? BioMed Research International |
author_facet |
Negin Anousha Arash Hossein-Nezhad Firouzeh Biramijamal Ali Rahmani Zhila Maghbooli Elahe Aghababaei Shahram Nemati |
author_sort |
Negin Anousha |
title |
Association Study of Estrogen Receptor Alpha Gene Polymorphisms with Spontaneous Abortion: Is This a Possible Reason for Unexplained Spontaneous Abortion? |
title_short |
Association Study of Estrogen Receptor Alpha Gene Polymorphisms with Spontaneous Abortion: Is This a Possible Reason for Unexplained Spontaneous Abortion? |
title_full |
Association Study of Estrogen Receptor Alpha Gene Polymorphisms with Spontaneous Abortion: Is This a Possible Reason for Unexplained Spontaneous Abortion? |
title_fullStr |
Association Study of Estrogen Receptor Alpha Gene Polymorphisms with Spontaneous Abortion: Is This a Possible Reason for Unexplained Spontaneous Abortion? |
title_full_unstemmed |
Association Study of Estrogen Receptor Alpha Gene Polymorphisms with Spontaneous Abortion: Is This a Possible Reason for Unexplained Spontaneous Abortion? |
title_sort |
association study of estrogen receptor alpha gene polymorphisms with spontaneous abortion: is this a possible reason for unexplained spontaneous abortion? |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2013-01-01 |
description |
Estrogen plays a crucial role in fetal and placental development through estrogen receptors. Association of estrogen receptor alpha gene (ESR1) polymorphisms with spontaneous abortion has been shown in some studies. Our main goal was to study the potential association of spontaneous abortion with the ESR1 gene variations (PvuII and XbaI) in fetal tissue. Totally, 161 samples were recruited including 80 samples of formalin-fixed paraffin-embedded fetal tissue from spontaneous abortion and 81 samples of normal term placental tissue. The restriction fragment length polymorphism (RFLP) method was performed for genotyping the rs2234693 (A/G XbaI) and rs9340799 (T/C PvuII) single nucleotide polymorphisms located in intron 1 of ESR1. The results have been confirmed by DNA sequencing analysis. The different genotypes distribution was detected in two study groups. Haplotype analysis indicated that ppxx is protective genotype against spontaneous abortion (P = 0.01). In conclusion, the potential role of ESR1 genetic variation in spontaneous abortion might be valuable in high-risk subjects, and that needs to be confirmed with future studies. |
url |
http://dx.doi.org/10.1155/2013/256470 |
work_keys_str_mv |
AT neginanousha associationstudyofestrogenreceptoralphagenepolymorphismswithspontaneousabortionisthisapossiblereasonforunexplainedspontaneousabortion AT arashhosseinnezhad associationstudyofestrogenreceptoralphagenepolymorphismswithspontaneousabortionisthisapossiblereasonforunexplainedspontaneousabortion AT firouzehbiramijamal associationstudyofestrogenreceptoralphagenepolymorphismswithspontaneousabortionisthisapossiblereasonforunexplainedspontaneousabortion AT alirahmani associationstudyofestrogenreceptoralphagenepolymorphismswithspontaneousabortionisthisapossiblereasonforunexplainedspontaneousabortion AT zhilamaghbooli associationstudyofestrogenreceptoralphagenepolymorphismswithspontaneousabortionisthisapossiblereasonforunexplainedspontaneousabortion AT elaheaghababaei associationstudyofestrogenreceptoralphagenepolymorphismswithspontaneousabortionisthisapossiblereasonforunexplainedspontaneousabortion AT shahramnemati associationstudyofestrogenreceptoralphagenepolymorphismswithspontaneousabortionisthisapossiblereasonforunexplainedspontaneousabortion |
_version_ |
1725133918924963840 |