Non-genetic Purification of Ventricular Cardiomyocytes from Differentiating Embryonic Stem Cells through Molecular Beacons Targeting IRX-4

Non-genetic Purification of Ventricular Cardiomyocytes from Differentiating Embryonic Stem Cells through Molecular Beacons Targeting IRX-4

Isolation of ventricular cardiomyocytes (vCMs) has been challenging due to the lack of specific surface markers. Here we show that vCMs can be purified from differentiating mouse embryonic stem cells (mESCs) using molecular beacons (MBs) targeting specific intracellular mRNAs. We designed MBs (IRX4...

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Bibliographic Details
Main Authors: Kiwon Ban, Brian Wile, Kyu-Won Cho, Sangsung Kim, Ming-Ke Song, Sang Yoon Kim, Jason Singer, Anum Syed, Shan Ping Yu, Mary Wagner, Gang Bao, Young-sup Yoon
Format: Article
Language:English
Published: Elsevier 2015-12-01
Series:Stem Cell Reports
Subjects:
pluripotent stem cell
ventricular cardiomyocyte
molecular beacons
cell selection
IRX4
Online Access:http://www.sciencedirect.com/science/article/pii/S2213671115003185

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http://www.sciencedirect.com/science/article/pii/S2213671115003185

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