Phospholipase D1 Ablation Disrupts Mouse Longitudinal Hippocampal Axis Organization and Functioning
Summary: Phosphatidic acid (PA) is a signaling lipid involved in the modulation of synaptic structure and functioning. Based on previous work showing a decreasing PA gradient along the longitudinal axis of the rodent hippocampus, we asked whether the dorsal hippocampus (DH) and the ventral hippocamp...
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Elsevier
2020-03-01
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Series: | Cell Reports |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124720302837 |
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Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Luísa Santa-Marinha Isabel Castanho Rita Ribeiro Silva Francisca Vaz Bravo André Miguel Miranda Torcato Meira Rafaela Morais-Ribeiro Fernanda Marques Yimeng Xu Kimberly Point du Jour Markus Wenk Robin Barry Chan Gilbert Di Paolo Vítor Pinto Tiago Gil Oliveira |
spellingShingle |
Luísa Santa-Marinha Isabel Castanho Rita Ribeiro Silva Francisca Vaz Bravo André Miguel Miranda Torcato Meira Rafaela Morais-Ribeiro Fernanda Marques Yimeng Xu Kimberly Point du Jour Markus Wenk Robin Barry Chan Gilbert Di Paolo Vítor Pinto Tiago Gil Oliveira Phospholipase D1 Ablation Disrupts Mouse Longitudinal Hippocampal Axis Organization and Functioning Cell Reports |
author_facet |
Luísa Santa-Marinha Isabel Castanho Rita Ribeiro Silva Francisca Vaz Bravo André Miguel Miranda Torcato Meira Rafaela Morais-Ribeiro Fernanda Marques Yimeng Xu Kimberly Point du Jour Markus Wenk Robin Barry Chan Gilbert Di Paolo Vítor Pinto Tiago Gil Oliveira |
author_sort |
Luísa Santa-Marinha |
title |
Phospholipase D1 Ablation Disrupts Mouse Longitudinal Hippocampal Axis Organization and Functioning |
title_short |
Phospholipase D1 Ablation Disrupts Mouse Longitudinal Hippocampal Axis Organization and Functioning |
title_full |
Phospholipase D1 Ablation Disrupts Mouse Longitudinal Hippocampal Axis Organization and Functioning |
title_fullStr |
Phospholipase D1 Ablation Disrupts Mouse Longitudinal Hippocampal Axis Organization and Functioning |
title_full_unstemmed |
Phospholipase D1 Ablation Disrupts Mouse Longitudinal Hippocampal Axis Organization and Functioning |
title_sort |
phospholipase d1 ablation disrupts mouse longitudinal hippocampal axis organization and functioning |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2020-03-01 |
description |
Summary: Phosphatidic acid (PA) is a signaling lipid involved in the modulation of synaptic structure and functioning. Based on previous work showing a decreasing PA gradient along the longitudinal axis of the rodent hippocampus, we asked whether the dorsal hippocampus (DH) and the ventral hippocampus (VH) are differentially affected by PA modulation. Here, we show that phospholipase D1 (PLD1) is a major hippocampal PA source, compared to PLD2, and that PLD1 ablation affects predominantly the lipidome of the DH. Moreover, Pld1 knockout (KO) mice show specific deficits in novel object recognition and social interaction and disruption in the DH-VH dendritic arborization differentiation in CA1/CA3 pyramidal neurons. Also, Pld1 KO animals present reduced long-term depression (LTD) induction and reduced GluN2A and SNAP-25 protein levels in the DH. Overall, we observe that PLD1-derived PA reduction leads to differential lipid signatures along the longitudinal hippocampal axis, predominantly affecting DH organization and functioning. : Santa-Marinha et al. show that PLD1 and PLD2 contribute differentially to the mouse hippocampal lipidomic profile. Their data highlight PLD1-derived PA reduction as a major modulator of dorsal-ventral hippocampal organization and functioning. Keywords: phospholipase D, PLD1, PLD2, dorsal hippocampus, ventral hippocampus, longitudinal hippocampal axis, lipids, lipidomics, long-term depression, social memory |
url |
http://www.sciencedirect.com/science/article/pii/S2211124720302837 |
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doaj-f1f21573562f4a339d1161bbd04a70402020-11-25T02:09:23ZengElsevierCell Reports2211-12472020-03-01301241974208.e6Phospholipase D1 Ablation Disrupts Mouse Longitudinal Hippocampal Axis Organization and FunctioningLuísa Santa-Marinha0Isabel Castanho1Rita Ribeiro Silva2Francisca Vaz Bravo3André Miguel Miranda4Torcato Meira5Rafaela Morais-Ribeiro6Fernanda Marques7Yimeng Xu8Kimberly Point du Jour9Markus Wenk10Robin Barry Chan11Gilbert Di Paolo12Vítor Pinto13Tiago Gil Oliveira14Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal; ICVS/3B’s - PT Government Associate Laboratory, Braga/Guimarães, PortugalLife and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal; ICVS/3B’s - PT Government Associate Laboratory, Braga/Guimarães, PortugalLife and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal; ICVS/3B’s - PT Government Associate Laboratory, Braga/Guimarães, PortugalLife and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal; ICVS/3B’s - PT Government Associate Laboratory, Braga/Guimarães, PortugalLife and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal; ICVS/3B’s - PT Government Associate Laboratory, Braga/Guimarães, PortugalLife and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal; ICVS/3B’s - PT Government Associate Laboratory, Braga/Guimarães, PortugalLife and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal; ICVS/3B’s - PT Government Associate Laboratory, Braga/Guimarães, PortugalLife and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal; ICVS/3B’s - PT Government Associate Laboratory, Braga/Guimarães, PortugalDepartment of Pathology and Cell Biology, Columbia University Medical Center, New York, NY 10032, USADepartment of Pathology and Cell Biology, Columbia University Medical Center, New York, NY 10032, USA; Taub Institute for Research on Alzheimer’s Disease and the Aging Brain, Columbia University Medical Center, New York, NY 10032, USADepartment of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117596, SingaporeDepartment of Pathology and Cell Biology, Columbia University Medical Center, New York, NY 10032, USA; Taub Institute for Research on Alzheimer’s Disease and the Aging Brain, Columbia University Medical Center, New York, NY 10032, USADepartment of Pathology and Cell Biology, Columbia University Medical Center, New York, NY 10032, USA; Taub Institute for Research on Alzheimer’s Disease and the Aging Brain, Columbia University Medical Center, New York, NY 10032, USALife and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal; ICVS/3B’s - PT Government Associate Laboratory, Braga/Guimarães, PortugalLife and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal; ICVS/3B’s - PT Government Associate Laboratory, Braga/Guimarães, Portugal; Corresponding authorSummary: Phosphatidic acid (PA) is a signaling lipid involved in the modulation of synaptic structure and functioning. Based on previous work showing a decreasing PA gradient along the longitudinal axis of the rodent hippocampus, we asked whether the dorsal hippocampus (DH) and the ventral hippocampus (VH) are differentially affected by PA modulation. Here, we show that phospholipase D1 (PLD1) is a major hippocampal PA source, compared to PLD2, and that PLD1 ablation affects predominantly the lipidome of the DH. Moreover, Pld1 knockout (KO) mice show specific deficits in novel object recognition and social interaction and disruption in the DH-VH dendritic arborization differentiation in CA1/CA3 pyramidal neurons. Also, Pld1 KO animals present reduced long-term depression (LTD) induction and reduced GluN2A and SNAP-25 protein levels in the DH. Overall, we observe that PLD1-derived PA reduction leads to differential lipid signatures along the longitudinal hippocampal axis, predominantly affecting DH organization and functioning. : Santa-Marinha et al. show that PLD1 and PLD2 contribute differentially to the mouse hippocampal lipidomic profile. Their data highlight PLD1-derived PA reduction as a major modulator of dorsal-ventral hippocampal organization and functioning. Keywords: phospholipase D, PLD1, PLD2, dorsal hippocampus, ventral hippocampus, longitudinal hippocampal axis, lipids, lipidomics, long-term depression, social memoryhttp://www.sciencedirect.com/science/article/pii/S2211124720302837 |