Monitoring Autophagy in the Model Green Microalga Chlamydomonas reinhardtii
Autophagy is an intracellular catabolic system that delivers cytoplasmic constituents and organelles in the vacuole. This degradative process is mediated by a group of proteins coded by autophagy-related (ATG) genes that are widely conserved from yeasts to plants and mammals. Homologs of ATG genes h...
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doaj-f1fa2880f6df4ac3bc07424a917d8b1f2020-11-24T21:48:04ZengMDPI AGCells2073-44092017-10-01643610.3390/cells6040036cells6040036Monitoring Autophagy in the Model Green Microalga Chlamydomonas reinhardtiiMaría Esther Pérez-Pérez0Inmaculada Couso1Luis G. Heredia-Martínez2José L. Crespo3Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas (CSIC)—Universidad de Sevilla; Avda. Américo Vespucio, 49, 41092 Sevilla, SpainInstituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas (CSIC)—Universidad de Sevilla; Avda. Américo Vespucio, 49, 41092 Sevilla, SpainInstituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas (CSIC)—Universidad de Sevilla; Avda. Américo Vespucio, 49, 41092 Sevilla, SpainInstituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas (CSIC)—Universidad de Sevilla; Avda. Américo Vespucio, 49, 41092 Sevilla, SpainAutophagy is an intracellular catabolic system that delivers cytoplasmic constituents and organelles in the vacuole. This degradative process is mediated by a group of proteins coded by autophagy-related (ATG) genes that are widely conserved from yeasts to plants and mammals. Homologs of ATG genes have been also identified in algal genomes including the unicellular model green alga Chlamydomonas reinhardtii. The development of specific tools to monitor autophagy in Chlamydomonas has expanded our current knowledge about the regulation and function of this process in algae. Recent findings indicated that autophagy is regulated by redox signals and the TOR network in Chlamydomonas and revealed that this process may play in important role in the control of lipid metabolism and ribosomal protein turnover in this alga. Here, we will describe the different techniques and approaches that have been reported to study autophagy and autophagic flux in Chlamydomonas.https://www.mdpi.com/2073-4409/6/4/36Chlamydomonasgreen algaautophagyautophagic fluxATG8redoxstress |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
María Esther Pérez-Pérez Inmaculada Couso Luis G. Heredia-Martínez José L. Crespo |
spellingShingle |
María Esther Pérez-Pérez Inmaculada Couso Luis G. Heredia-Martínez José L. Crespo Monitoring Autophagy in the Model Green Microalga Chlamydomonas reinhardtii Cells Chlamydomonas green alga autophagy autophagic flux ATG8 redox stress |
author_facet |
María Esther Pérez-Pérez Inmaculada Couso Luis G. Heredia-Martínez José L. Crespo |
author_sort |
María Esther Pérez-Pérez |
title |
Monitoring Autophagy in the Model Green Microalga Chlamydomonas reinhardtii |
title_short |
Monitoring Autophagy in the Model Green Microalga Chlamydomonas reinhardtii |
title_full |
Monitoring Autophagy in the Model Green Microalga Chlamydomonas reinhardtii |
title_fullStr |
Monitoring Autophagy in the Model Green Microalga Chlamydomonas reinhardtii |
title_full_unstemmed |
Monitoring Autophagy in the Model Green Microalga Chlamydomonas reinhardtii |
title_sort |
monitoring autophagy in the model green microalga chlamydomonas reinhardtii |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2017-10-01 |
description |
Autophagy is an intracellular catabolic system that delivers cytoplasmic constituents and organelles in the vacuole. This degradative process is mediated by a group of proteins coded by autophagy-related (ATG) genes that are widely conserved from yeasts to plants and mammals. Homologs of ATG genes have been also identified in algal genomes including the unicellular model green alga Chlamydomonas reinhardtii. The development of specific tools to monitor autophagy in Chlamydomonas has expanded our current knowledge about the regulation and function of this process in algae. Recent findings indicated that autophagy is regulated by redox signals and the TOR network in Chlamydomonas and revealed that this process may play in important role in the control of lipid metabolism and ribosomal protein turnover in this alga. Here, we will describe the different techniques and approaches that have been reported to study autophagy and autophagic flux in Chlamydomonas. |
topic |
Chlamydomonas green alga autophagy autophagic flux ATG8 redox stress |
url |
https://www.mdpi.com/2073-4409/6/4/36 |
work_keys_str_mv |
AT mariaestherperezperez monitoringautophagyinthemodelgreenmicroalgachlamydomonasreinhardtii AT inmaculadacouso monitoringautophagyinthemodelgreenmicroalgachlamydomonasreinhardtii AT luisgherediamartinez monitoringautophagyinthemodelgreenmicroalgachlamydomonasreinhardtii AT joselcrespo monitoringautophagyinthemodelgreenmicroalgachlamydomonasreinhardtii |
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