Influenza Virus-like Particle (VLP) Vaccines Expressing the SARS-CoV-2 S Glycoprotein, S1, or S2 Domains
The ongoing severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic had brought disastrous consequences throughout the entire world. While several manufactured vaccines have been approved for emergency use, continuous efforts to generate novel vaccines are needed. In this study, we dev...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-08-01
|
Series: | Vaccines |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-393X/9/8/920 |
id |
doaj-f201233a532743bf8ddf1eeb607dc2d5 |
---|---|
record_format |
Article |
spelling |
doaj-f201233a532743bf8ddf1eeb607dc2d52021-08-26T14:25:58ZengMDPI AGVaccines2076-393X2021-08-01992092010.3390/vaccines9080920Influenza Virus-like Particle (VLP) Vaccines Expressing the SARS-CoV-2 S Glycoprotein, S1, or S2 DomainsKi-Back Chu0Hae-Ji Kang1Keon-Woong Yoon2Hae-Ahm Lee3Eun-Kyung Moon4Beom-Ku Han5Fu-Shi Quan6Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, KoreaMedical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Graduate School, Kyung Hee University, Seoul 02447, KoreaDepartment of Medical Zoology, School of Medicine, Kyung Hee University, Seoul 02447, KoreaOptipharm Inc., Cheongju 28158, KoreaMedical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Graduate School, Kyung Hee University, Seoul 02447, KoreaThe ongoing severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic had brought disastrous consequences throughout the entire world. While several manufactured vaccines have been approved for emergency use, continuous efforts to generate novel vaccines are needed. In this study, we developed SARS-CoV-2 virus-like particles (VLPs) containing the full length of spike (S) glycoprotein (S full), S1, or S2 together with the influenza matrix protein 1 (M1) as a core protein. Successfully constructed VLPs expressing the S full, S1, and S2 via Sf9 cell transfections were confirmed and characterized by Western blot and transmission electron microscopy (TEM). VLP immunization in mice induced higher levels of spike protein-specific IgG and its subclasses compared to naïve control, with IgG2a being the most predominant subclass. S full and S1 immune sera elicited virus-neutralizing activities, but these were not strong enough to fully inhibit receptor–ligand binding of the SARS-CoV-2. Neutralizing activities were not observed from the S2 VLP immune sera. Overall, our findings revealed that S full or S1 containing VLPs can be developed into effective vaccines.https://www.mdpi.com/2076-393X/9/8/920COVID-19SARS-CoV-2virus-like particlevaccineantibodyneutralization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ki-Back Chu Hae-Ji Kang Keon-Woong Yoon Hae-Ahm Lee Eun-Kyung Moon Beom-Ku Han Fu-Shi Quan |
spellingShingle |
Ki-Back Chu Hae-Ji Kang Keon-Woong Yoon Hae-Ahm Lee Eun-Kyung Moon Beom-Ku Han Fu-Shi Quan Influenza Virus-like Particle (VLP) Vaccines Expressing the SARS-CoV-2 S Glycoprotein, S1, or S2 Domains Vaccines COVID-19 SARS-CoV-2 virus-like particle vaccine antibody neutralization |
author_facet |
Ki-Back Chu Hae-Ji Kang Keon-Woong Yoon Hae-Ahm Lee Eun-Kyung Moon Beom-Ku Han Fu-Shi Quan |
author_sort |
Ki-Back Chu |
title |
Influenza Virus-like Particle (VLP) Vaccines Expressing the SARS-CoV-2 S Glycoprotein, S1, or S2 Domains |
title_short |
Influenza Virus-like Particle (VLP) Vaccines Expressing the SARS-CoV-2 S Glycoprotein, S1, or S2 Domains |
title_full |
Influenza Virus-like Particle (VLP) Vaccines Expressing the SARS-CoV-2 S Glycoprotein, S1, or S2 Domains |
title_fullStr |
Influenza Virus-like Particle (VLP) Vaccines Expressing the SARS-CoV-2 S Glycoprotein, S1, or S2 Domains |
title_full_unstemmed |
Influenza Virus-like Particle (VLP) Vaccines Expressing the SARS-CoV-2 S Glycoprotein, S1, or S2 Domains |
title_sort |
influenza virus-like particle (vlp) vaccines expressing the sars-cov-2 s glycoprotein, s1, or s2 domains |
publisher |
MDPI AG |
series |
Vaccines |
issn |
2076-393X |
publishDate |
2021-08-01 |
description |
The ongoing severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic had brought disastrous consequences throughout the entire world. While several manufactured vaccines have been approved for emergency use, continuous efforts to generate novel vaccines are needed. In this study, we developed SARS-CoV-2 virus-like particles (VLPs) containing the full length of spike (S) glycoprotein (S full), S1, or S2 together with the influenza matrix protein 1 (M1) as a core protein. Successfully constructed VLPs expressing the S full, S1, and S2 via Sf9 cell transfections were confirmed and characterized by Western blot and transmission electron microscopy (TEM). VLP immunization in mice induced higher levels of spike protein-specific IgG and its subclasses compared to naïve control, with IgG2a being the most predominant subclass. S full and S1 immune sera elicited virus-neutralizing activities, but these were not strong enough to fully inhibit receptor–ligand binding of the SARS-CoV-2. Neutralizing activities were not observed from the S2 VLP immune sera. Overall, our findings revealed that S full or S1 containing VLPs can be developed into effective vaccines. |
topic |
COVID-19 SARS-CoV-2 virus-like particle vaccine antibody neutralization |
url |
https://www.mdpi.com/2076-393X/9/8/920 |
work_keys_str_mv |
AT kibackchu influenzaviruslikeparticlevlpvaccinesexpressingthesarscov2sglycoproteins1ors2domains AT haejikang influenzaviruslikeparticlevlpvaccinesexpressingthesarscov2sglycoproteins1ors2domains AT keonwoongyoon influenzaviruslikeparticlevlpvaccinesexpressingthesarscov2sglycoproteins1ors2domains AT haeahmlee influenzaviruslikeparticlevlpvaccinesexpressingthesarscov2sglycoproteins1ors2domains AT eunkyungmoon influenzaviruslikeparticlevlpvaccinesexpressingthesarscov2sglycoproteins1ors2domains AT beomkuhan influenzaviruslikeparticlevlpvaccinesexpressingthesarscov2sglycoproteins1ors2domains AT fushiquan influenzaviruslikeparticlevlpvaccinesexpressingthesarscov2sglycoproteins1ors2domains |
_version_ |
1721189540340695040 |