Quercetin protects islet β‑cells from oxidation-induced apoptosis via Sirt3 in T2DM
Objective(s): Sirt3 may regulate ROS production and might be involved in β‑cell apoptosis, which plays an important role in the progression of type 2 diabetes mellitus (T2DM). Quercetin is a potent anti-oxidative bioflavonoid, but its effects on T2DM remain to be explored. This study aimed to invest...
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doaj-f21bcb7d25b54b108f6cd083fed5ca482021-06-27T06:26:54ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences 2008-38662008-38742021-05-0124562963510.22038/ijbms.2021.52005.1179217878Quercetin protects islet β‑cells from oxidation-induced apoptosis via Sirt3 in T2DMJian-Yun Wang0Ya-Xing Nie1Bing-Zheng Dong2Zhi-Chen Cai3Xuan-Kai Zeng4Lei Du5Xia Zhu6Xiao-Xing Yin7Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221002, Jiangsu, PR ChinaJiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221002, Jiangsu, PR ChinaDepartment of Urology, Xuzhou Central Hospital, The Affiliated School of Clinical Medicine of Xuzhou Medical University, Xuzhou 221009, Jiangsu, PR ChinaJiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221002, Jiangsu, PR ChinaJiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221002, Jiangsu, PR ChinaJiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221002, Jiangsu, PR ChinaJiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221002, Jiangsu, PR ChinaJiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221002, Jiangsu, PR ChinaObjective(s): Sirt3 may regulate ROS production and might be involved in β‑cell apoptosis, which plays an important role in the progression of type 2 diabetes mellitus (T2DM). Quercetin is a potent anti-oxidative bioflavonoid, but its effects on T2DM remain to be explored. This study aimed to investigate the effects of quercetin on β‑cell apoptosis and explore its mechanisms.Materials and Methods: The effects of quercetin were conducted on db/db mice and INS1 cells. Fasting blood glucose was determined by the colorimetric method, serum insulin was measured by enzyme‑linked immunosorbent assay (ELISA). Meanwhile, Sirt3 in INS1 cells was knocked down by plasmid transfection. The antioxidant proteins (SOD2 and CAT), apoptosis proteins (cleaved Caspase-3, Bax, and BCL-2), and Sirt3 protein in pancreases and INS1 cells were determined by western blotting. Results: When INS1 cells and diabetic mice were treated with quercetin, the levels of SOD2, CAT, and Sirt3 proteins were increased, the levels of cleaved Caspase-3 and the ratio of Bax to BCL-2 were decreased at different degrees, along with reduced blood glucose levels and elevated insulin levels in diabetic mice. When Sirt3 was knocked down in INS1 cells, increase of two antioxidants and decrease of cell apoptosis generated by quercetin could not occur. Conclusion: Quercetin protected islet β‑cells from oxidation-induced apoptosis via Sirt3 in T2DM, which would be beneficial to develop new strategies for preventing β‑cell failure in T2DM.https://ijbms.mums.ac.ir/article_17878_07cdeeb64f4b5ad398be397df81700ab.pdfapoptosisβ‑celloxidative stressquercetinsirt3 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jian-Yun Wang Ya-Xing Nie Bing-Zheng Dong Zhi-Chen Cai Xuan-Kai Zeng Lei Du Xia Zhu Xiao-Xing Yin |
spellingShingle |
Jian-Yun Wang Ya-Xing Nie Bing-Zheng Dong Zhi-Chen Cai Xuan-Kai Zeng Lei Du Xia Zhu Xiao-Xing Yin Quercetin protects islet β‑cells from oxidation-induced apoptosis via Sirt3 in T2DM Iranian Journal of Basic Medical Sciences apoptosis β‑cell oxidative stress quercetin sirt3 |
author_facet |
Jian-Yun Wang Ya-Xing Nie Bing-Zheng Dong Zhi-Chen Cai Xuan-Kai Zeng Lei Du Xia Zhu Xiao-Xing Yin |
author_sort |
Jian-Yun Wang |
title |
Quercetin protects islet β‑cells from oxidation-induced apoptosis via Sirt3 in T2DM |
title_short |
Quercetin protects islet β‑cells from oxidation-induced apoptosis via Sirt3 in T2DM |
title_full |
Quercetin protects islet β‑cells from oxidation-induced apoptosis via Sirt3 in T2DM |
title_fullStr |
Quercetin protects islet β‑cells from oxidation-induced apoptosis via Sirt3 in T2DM |
title_full_unstemmed |
Quercetin protects islet β‑cells from oxidation-induced apoptosis via Sirt3 in T2DM |
title_sort |
quercetin protects islet β‑cells from oxidation-induced apoptosis via sirt3 in t2dm |
publisher |
Mashhad University of Medical Sciences |
series |
Iranian Journal of Basic Medical Sciences |
issn |
2008-3866 2008-3874 |
publishDate |
2021-05-01 |
description |
Objective(s): Sirt3 may regulate ROS production and might be involved in β‑cell apoptosis, which plays an important role in the progression of type 2 diabetes mellitus (T2DM). Quercetin is a potent anti-oxidative bioflavonoid, but its effects on T2DM remain to be explored. This study aimed to investigate the effects of quercetin on β‑cell apoptosis and explore its mechanisms.Materials and Methods: The effects of quercetin were conducted on db/db mice and INS1 cells. Fasting blood glucose was determined by the colorimetric method, serum insulin was measured by enzyme‑linked immunosorbent assay (ELISA). Meanwhile, Sirt3 in INS1 cells was knocked down by plasmid transfection. The antioxidant proteins (SOD2 and CAT), apoptosis proteins (cleaved Caspase-3, Bax, and BCL-2), and Sirt3 protein in pancreases and INS1 cells were determined by western blotting. Results: When INS1 cells and diabetic mice were treated with quercetin, the levels of SOD2, CAT, and Sirt3 proteins were increased, the levels of cleaved Caspase-3 and the ratio of Bax to BCL-2 were decreased at different degrees, along with reduced blood glucose levels and elevated insulin levels in diabetic mice. When Sirt3 was knocked down in INS1 cells, increase of two antioxidants and decrease of cell apoptosis generated by quercetin could not occur. Conclusion: Quercetin protected islet β‑cells from oxidation-induced apoptosis via Sirt3 in T2DM, which would be beneficial to develop new strategies for preventing β‑cell failure in T2DM. |
topic |
apoptosis β‑cell oxidative stress quercetin sirt3 |
url |
https://ijbms.mums.ac.ir/article_17878_07cdeeb64f4b5ad398be397df81700ab.pdf |
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