Assessment of global DNA methylation in children with tuberculosis disease
Background: Studying DNA methylation changes in disease condition provides the basis of disease pathogenesis or host immune response to infection. Evidences suggest that pathogen-mediated DNA methylation changes influences the expression pattern of genes contributing to disease condition to avert ho...
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doaj-f22a298eb19641ccb7e2b2be047918182020-11-25T00:40:27ZengWolters Kluwer Medknow PublicationsInternational Journal of Mycobacteriology2212-55312212-554X2018-01-017433834210.4103/ijmy.ijmy_107_18Assessment of global DNA methylation in children with tuberculosis diseaseKathirvel MaruthaiEllappan KalaiarasanNoyal Mariya JosephSubhash Chandra ParijaSubramanian MahadevanBackground: Studying DNA methylation changes in disease condition provides the basis of disease pathogenesis or host immune response to infection. Evidences suggest that pathogen-mediated DNA methylation changes influences the expression pattern of genes contributing to disease condition to avert host immune response. Hence, we attempted to study the association between tubercle bacilli-mediated global DNA methylation changes in children with tuberculosis (TB) disease and healthy controls. Methods: Forty-three children diagnosed with TB and 33 healthy children were enrolled in this study. ELISA-based global DNA methylation quantification was performed to measure the changes in percentage of global genomic DNA methylation level. Results: Highly significant difference in global DNA methylation level was found between cases and controls and median global DNA methylation level was 6.25% (interquartile range [IQR] 2.5%–10%) in cases and 25% (IQR, 12.5%–25%) in controls (P < 0.001). Significant difference was found in GeneXpert-positive cases (P < 0.01). Receiver operating curve analysis showed that area under curve 0.81 for the total study population and 0.76 for GeneXpert-positive cases. Conclusion: The results show the significant difference in global DNA methylation level in children with TB disease that can serve as a potential biomarker in early diagnosis of TB disease. Measuring global DNA methylation level, however, not an accurate or sensitive diagnostic method but evaluating active demethylation at genome-wide level can be used to monitor disease progression and treatment efficacy.http://www.ijmyco.org/article.asp?issn=2212-5531;year=2018;volume=7;issue=4;spage=338;epage=342;aulast=Maruthai5-mC quantificationDNA Methylationhypomethylationtuberculosis diagnosistuberculosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kathirvel Maruthai Ellappan Kalaiarasan Noyal Mariya Joseph Subhash Chandra Parija Subramanian Mahadevan |
spellingShingle |
Kathirvel Maruthai Ellappan Kalaiarasan Noyal Mariya Joseph Subhash Chandra Parija Subramanian Mahadevan Assessment of global DNA methylation in children with tuberculosis disease International Journal of Mycobacteriology 5-mC quantification DNA Methylation hypomethylation tuberculosis diagnosis tuberculosis |
author_facet |
Kathirvel Maruthai Ellappan Kalaiarasan Noyal Mariya Joseph Subhash Chandra Parija Subramanian Mahadevan |
author_sort |
Kathirvel Maruthai |
title |
Assessment of global DNA methylation in children with tuberculosis disease |
title_short |
Assessment of global DNA methylation in children with tuberculosis disease |
title_full |
Assessment of global DNA methylation in children with tuberculosis disease |
title_fullStr |
Assessment of global DNA methylation in children with tuberculosis disease |
title_full_unstemmed |
Assessment of global DNA methylation in children with tuberculosis disease |
title_sort |
assessment of global dna methylation in children with tuberculosis disease |
publisher |
Wolters Kluwer Medknow Publications |
series |
International Journal of Mycobacteriology |
issn |
2212-5531 2212-554X |
publishDate |
2018-01-01 |
description |
Background: Studying DNA methylation changes in disease condition provides the basis of disease pathogenesis or host immune response to infection. Evidences suggest that pathogen-mediated DNA methylation changes influences the expression pattern of genes contributing to disease condition to avert host immune response. Hence, we attempted to study the association between tubercle bacilli-mediated global DNA methylation changes in children with tuberculosis (TB) disease and healthy controls. Methods: Forty-three children diagnosed with TB and 33 healthy children were enrolled in this study. ELISA-based global DNA methylation quantification was performed to measure the changes in percentage of global genomic DNA methylation level. Results: Highly significant difference in global DNA methylation level was found between cases and controls and median global DNA methylation level was 6.25% (interquartile range [IQR] 2.5%–10%) in cases and 25% (IQR, 12.5%–25%) in controls (P < 0.001). Significant difference was found in GeneXpert-positive cases (P < 0.01). Receiver operating curve analysis showed that area under curve 0.81 for the total study population and 0.76 for GeneXpert-positive cases. Conclusion: The results show the significant difference in global DNA methylation level in children with TB disease that can serve as a potential biomarker in early diagnosis of TB disease. Measuring global DNA methylation level, however, not an accurate or sensitive diagnostic method but evaluating active demethylation at genome-wide level can be used to monitor disease progression and treatment efficacy. |
topic |
5-mC quantification DNA Methylation hypomethylation tuberculosis diagnosis tuberculosis |
url |
http://www.ijmyco.org/article.asp?issn=2212-5531;year=2018;volume=7;issue=4;spage=338;epage=342;aulast=Maruthai |
work_keys_str_mv |
AT kathirvelmaruthai assessmentofglobaldnamethylationinchildrenwithtuberculosisdisease AT ellappankalaiarasan assessmentofglobaldnamethylationinchildrenwithtuberculosisdisease AT noyalmariyajoseph assessmentofglobaldnamethylationinchildrenwithtuberculosisdisease AT subhashchandraparija assessmentofglobaldnamethylationinchildrenwithtuberculosisdisease AT subramanianmahadevan assessmentofglobaldnamethylationinchildrenwithtuberculosisdisease |
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