DIAGNOSTIC ACCURACY OF IGA ANTI-TISSUE TRANSGLUTAMINASE ANTIBODY IN THE DIAGNOSIS OF CELIAC DISEASE TAKING HISTOPATHOLOGY AS GOLD STANDARD
Objective: To determine the diagnostic accuracy of IgA anti-tissue transglutaminase antibody in the diagnosis of celiac disease taking histopathology as gold standard. Study Design: Cross-sectional validation study. Place and Duration of Study: Department of Pediatrics, Pak Emirates Military H...
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doaj-f248bd5cb3e24451b3b01c653c429ecb2021-08-26T09:58:01ZengThe Army PressPakistan Armed Forces Medical Journal0030-96482411-88422021-04-01712418216655DIAGNOSTIC ACCURACY OF IGA ANTI-TISSUE TRANSGLUTAMINASE ANTIBODY IN THE DIAGNOSIS OF CELIAC DISEASE TAKING HISTOPATHOLOGY AS GOLD STANDARDMunir Akmal Lodhi0Zeeshan Saleem1Ammarah Ayub2Tehmina Munir3Shamama Hassan4Pak Emirates Military Hospital/National University of Medical Sciences (NUMS) Rawalpindi PakistanPak Emirates Military Hospital/National University of Medical Sciences (NUMS) Rawalpindi PakistanPak Emirates Military Hospital/National University of Medical Sciences (NUMS) Rawalpindi PakistanArmy Medical College/National University of Medical Sciences (NUMS) Rawalpindi PakistanPak Emirates Military Hospital/National University of Medical Sciences (NUMS) Rawalpindi PakistanObjective: To determine the diagnostic accuracy of IgA anti-tissue transglutaminase antibody in the diagnosis of celiac disease taking histopathology as gold standard. Study Design: Cross-sectional validation study. Place and Duration of Study: Department of Pediatrics, Pak Emirates Military Hospital Rawalpindi, from Apr 2015 to Jul 2016. Methodology: Ninety five consecutive children presenting with suspicion of celiac disease were included into this study after taking written informed consent. A predesigned proforma was used to record patient’s demographic details. Anti-tTG level of ≥25 U/ml was taken as diagnostic of celiac disease while results of histopathology on endoscopic biopsy were taken as gold standard. Results: The mean age of the patients was 6.48 ± 3.20 years and majority 53 (55.8%) of the children were aged between 5-10 years. There were 47 (49.5%) male and 48 (50.5%) female children in the study group. The serum Anti-tTG level ranged from 8.0 U/ml to 759.0 U/ml with a mean of 298.75 ± 225.51 U/ml. Taking a cut-off value of ≥25 U/ml for anti-tTG, 81 (85.3%) children were suspected of celiac disease. Histopathology of endoscopic biopsy confirmed celiac disease in 68 (71.6%) children with 62 true positive, 19 false positive, 6 false negative and 8 true negative cases. It yielded 91.18% sensitivity, 29.63% specificity and 73.68% accuracy for anti-tTG (≥25 U/ml) in the diagnosis of celiac disease with positive and negative predictive values of 76.54% and 57.14% respectively. Conclusion: IgA anti-tissue transglutaminase antibody (≥25 U/ml) was found to be 91.18% sensitive, 29.63% specific and 73.68% accurate in the diagnosis of celiac disease taking histopathology as gold standard with positive and negative predictive values of 76.54% and 57.14% respectively.https://pafmj.org/index.php/PAFMJ/article/view/6655anti-tissue transglutaminase antibodyceliac diseasediagnostic accuracy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Munir Akmal Lodhi Zeeshan Saleem Ammarah Ayub Tehmina Munir Shamama Hassan |
spellingShingle |
Munir Akmal Lodhi Zeeshan Saleem Ammarah Ayub Tehmina Munir Shamama Hassan DIAGNOSTIC ACCURACY OF IGA ANTI-TISSUE TRANSGLUTAMINASE ANTIBODY IN THE DIAGNOSIS OF CELIAC DISEASE TAKING HISTOPATHOLOGY AS GOLD STANDARD Pakistan Armed Forces Medical Journal anti-tissue transglutaminase antibody celiac disease diagnostic accuracy |
author_facet |
Munir Akmal Lodhi Zeeshan Saleem Ammarah Ayub Tehmina Munir Shamama Hassan |
author_sort |
Munir Akmal Lodhi |
title |
DIAGNOSTIC ACCURACY OF IGA ANTI-TISSUE TRANSGLUTAMINASE ANTIBODY IN THE DIAGNOSIS OF CELIAC DISEASE TAKING HISTOPATHOLOGY AS GOLD STANDARD |
title_short |
DIAGNOSTIC ACCURACY OF IGA ANTI-TISSUE TRANSGLUTAMINASE ANTIBODY IN THE DIAGNOSIS OF CELIAC DISEASE TAKING HISTOPATHOLOGY AS GOLD STANDARD |
title_full |
DIAGNOSTIC ACCURACY OF IGA ANTI-TISSUE TRANSGLUTAMINASE ANTIBODY IN THE DIAGNOSIS OF CELIAC DISEASE TAKING HISTOPATHOLOGY AS GOLD STANDARD |
title_fullStr |
DIAGNOSTIC ACCURACY OF IGA ANTI-TISSUE TRANSGLUTAMINASE ANTIBODY IN THE DIAGNOSIS OF CELIAC DISEASE TAKING HISTOPATHOLOGY AS GOLD STANDARD |
title_full_unstemmed |
DIAGNOSTIC ACCURACY OF IGA ANTI-TISSUE TRANSGLUTAMINASE ANTIBODY IN THE DIAGNOSIS OF CELIAC DISEASE TAKING HISTOPATHOLOGY AS GOLD STANDARD |
title_sort |
diagnostic accuracy of iga anti-tissue transglutaminase antibody in the diagnosis of celiac disease taking histopathology as gold standard |
publisher |
The Army Press |
series |
Pakistan Armed Forces Medical Journal |
issn |
0030-9648 2411-8842 |
publishDate |
2021-04-01 |
description |
Objective: To determine the diagnostic accuracy of IgA anti-tissue transglutaminase antibody in the diagnosis of celiac disease taking histopathology as gold standard.
Study Design: Cross-sectional validation study.
Place and Duration of Study: Department of Pediatrics, Pak Emirates Military Hospital Rawalpindi, from Apr 2015 to Jul 2016.
Methodology: Ninety five consecutive children presenting with suspicion of celiac disease were included into this study after taking written informed consent. A predesigned proforma was used to record patient’s demographic details. Anti-tTG level of ≥25 U/ml was taken as diagnostic of celiac disease while results of histopathology on endoscopic biopsy were taken as gold standard.
Results: The mean age of the patients was 6.48 ± 3.20 years and majority 53 (55.8%) of the children were aged between 5-10 years. There were 47 (49.5%) male and 48 (50.5%) female children in the study group. The serum Anti-tTG level ranged from 8.0 U/ml to 759.0 U/ml with a mean of 298.75 ± 225.51 U/ml. Taking a cut-off value of ≥25 U/ml for anti-tTG, 81 (85.3%)
children were suspected of celiac disease. Histopathology of endoscopic biopsy confirmed celiac disease in 68 (71.6%) children with 62 true positive, 19 false positive, 6 false negative and 8 true negative cases. It yielded 91.18% sensitivity, 29.63% specificity and 73.68% accuracy for anti-tTG (≥25 U/ml) in the diagnosis of celiac disease with positive and negative predictive
values of 76.54% and 57.14% respectively.
Conclusion: IgA anti-tissue transglutaminase antibody (≥25 U/ml) was found to be 91.18% sensitive, 29.63% specific and 73.68% accurate in the diagnosis of celiac disease taking histopathology as gold standard with positive and negative predictive values of 76.54% and 57.14% respectively. |
topic |
anti-tissue transglutaminase antibody celiac disease diagnostic accuracy |
url |
https://pafmj.org/index.php/PAFMJ/article/view/6655 |
work_keys_str_mv |
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