Identification and abiotic stress response of a glutamine synthetase gene (AccGS) from the Asiatic honeybee, Apis cerana cerana (Hymenoptera: Apidae)
Glutamine synthetase (GS) is an essential detoxification enzyme that plays an important role in stress responses; however, little information regarding the function of this enzyme in hymenopteran insects is available. In the present study, we isolated and characterized the gene encoding GS in the As...
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Institute of Entomology, Biology Centre, Czech Academy of Science
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doaj-f2531efb4e474672b88f245863750c5c2021-04-16T20:35:23ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292014-01-0111111910.14411/eje.2014.001eje-201401-0001Identification and abiotic stress response of a glutamine synthetase gene (AccGS) from the Asiatic honeybee, Apis cerana cerana (Hymenoptera: Apidae)Xiuling WANG0Yuzhen LI1Yan YAN2Baohua XU3Xingqi GUO4College of Life Sciences andCollege of Life Sciences andCollege of Life Sciences andCollege of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, 271018, P.R. China; e-mails: seabiscuit_616@126.com (X. Wang), liyuzhen1988@163.com (Y. Li), sdytyanyan@163.com (Y. Yan), xqguo@sdau.edu.cn (X. Guo), bhxu@sdau.edu.cn (B. Xu)College of Life Sciences andGlutamine synthetase (GS) is an essential detoxification enzyme that plays an important role in stress responses; however, little information regarding the function of this enzyme in hymenopteran insects is available. In the present study, we isolated and characterized the gene encoding GS in the Asiatic honeybee, Apis cerana cerana. Multiple alignments and a phylogenetic analysis of GS sequences showed that AccGS belongs to the GSII superfamily and clusters with invertebrate GSs. Real-time quantitative PCR data demonstrated that AccGS is expressed at all developmental stages and in all tissues, with the highest expression observed in the sixth larval instar and in the brain. Moreover, AccGS expression is highly regulated by environmental stress, including xenobiotic, temperature, and ultraviolet light stresses. A disc diffusion assay showed that the recombinant AccGS protein confers resistance to mercuric chloride (HgCl2) stress in E. coli. This suggests that AccGS may play multiple roles in early development and in environmental stress responses.https://www.eje.cz/artkey/eje-201401-0001_Identification_and_abiotic_stress_response_of_a_glutamine_synthetase_gene_AccGS_from_the_Asiatic_honeybee_Ap.phphymenopteraapidaeapis cerana ceranaglutamine synthetaseexpression analysisstress response |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiuling WANG Yuzhen LI Yan YAN Baohua XU Xingqi GUO |
spellingShingle |
Xiuling WANG Yuzhen LI Yan YAN Baohua XU Xingqi GUO Identification and abiotic stress response of a glutamine synthetase gene (AccGS) from the Asiatic honeybee, Apis cerana cerana (Hymenoptera: Apidae) European Journal of Entomology hymenoptera apidae apis cerana cerana glutamine synthetase expression analysis stress response |
author_facet |
Xiuling WANG Yuzhen LI Yan YAN Baohua XU Xingqi GUO |
author_sort |
Xiuling WANG |
title |
Identification and abiotic stress response of a glutamine synthetase gene (AccGS) from the Asiatic honeybee, Apis cerana cerana (Hymenoptera: Apidae) |
title_short |
Identification and abiotic stress response of a glutamine synthetase gene (AccGS) from the Asiatic honeybee, Apis cerana cerana (Hymenoptera: Apidae) |
title_full |
Identification and abiotic stress response of a glutamine synthetase gene (AccGS) from the Asiatic honeybee, Apis cerana cerana (Hymenoptera: Apidae) |
title_fullStr |
Identification and abiotic stress response of a glutamine synthetase gene (AccGS) from the Asiatic honeybee, Apis cerana cerana (Hymenoptera: Apidae) |
title_full_unstemmed |
Identification and abiotic stress response of a glutamine synthetase gene (AccGS) from the Asiatic honeybee, Apis cerana cerana (Hymenoptera: Apidae) |
title_sort |
identification and abiotic stress response of a glutamine synthetase gene (accgs) from the asiatic honeybee, apis cerana cerana (hymenoptera: apidae) |
publisher |
Institute of Entomology, Biology Centre, Czech Academy of Science |
series |
European Journal of Entomology |
issn |
1210-5759 1802-8829 |
publishDate |
2014-01-01 |
description |
Glutamine synthetase (GS) is an essential detoxification enzyme that plays an important role in stress responses; however, little information regarding the function of this enzyme in hymenopteran insects is available. In the present study, we isolated and characterized the gene encoding GS in the Asiatic honeybee, Apis cerana cerana. Multiple alignments and a phylogenetic analysis of GS sequences showed that AccGS belongs to the GSII superfamily and clusters with invertebrate GSs. Real-time quantitative PCR data demonstrated that AccGS is expressed at all developmental stages and in all tissues, with the highest expression observed in the sixth larval instar and in the brain. Moreover, AccGS expression is highly regulated by environmental stress, including xenobiotic, temperature, and ultraviolet light stresses. A disc diffusion assay showed that the recombinant AccGS protein confers resistance to mercuric chloride (HgCl2) stress in E. coli. This suggests that AccGS may play multiple roles in early development and in environmental stress responses. |
topic |
hymenoptera apidae apis cerana cerana glutamine synthetase expression analysis stress response |
url |
https://www.eje.cz/artkey/eje-201401-0001_Identification_and_abiotic_stress_response_of_a_glutamine_synthetase_gene_AccGS_from_the_Asiatic_honeybee_Ap.php |
work_keys_str_mv |
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