Sphingosine 1-Phosphate- and C-C Chemokine Receptor 2-Dependent Activation of CD4+ Plasmacytoid Dendritic Cells in the Bone Marrow Contributes to Signs of Sepsis-Induced Immunosuppression
Sepsis is the dysregulated response of the host to systemic, mostly bacterial infection, and is associated with an enhanced susceptibility to life-threatening opportunistic infections. During polymicrobial sepsis, dendritic cells (DCs) secrete enhanced levels of interleukin (IL) 10 due to an altered...
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Format: | Article |
Language: | English |
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Frontiers Media S.A.
2017-11-01
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Series: | Frontiers in Immunology |
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Online Access: | http://journal.frontiersin.org/article/10.3389/fimmu.2017.01622/full |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anna Smirnov Stephanie Pohlmann Melanie Nehring Shafaqat Ali Shafaqat Ali Ritu Mann-Nüttel Stefanie Scheu Anne-Charlotte Antoni Wiebke Hansen Manuela Büettner Miriam J. Gardiasch Astrid M. Westendorf Florian Wirsdörfer Eva Pastille Marcel Dudda Stefanie B. Flohé |
spellingShingle |
Anna Smirnov Stephanie Pohlmann Melanie Nehring Shafaqat Ali Shafaqat Ali Ritu Mann-Nüttel Stefanie Scheu Anne-Charlotte Antoni Wiebke Hansen Manuela Büettner Miriam J. Gardiasch Astrid M. Westendorf Florian Wirsdörfer Eva Pastille Marcel Dudda Stefanie B. Flohé Sphingosine 1-Phosphate- and C-C Chemokine Receptor 2-Dependent Activation of CD4+ Plasmacytoid Dendritic Cells in the Bone Marrow Contributes to Signs of Sepsis-Induced Immunosuppression Frontiers in Immunology dendritic cells bone marrow differentiation polymicrobial sepsis immunosuppression monocytes |
author_facet |
Anna Smirnov Stephanie Pohlmann Melanie Nehring Shafaqat Ali Shafaqat Ali Ritu Mann-Nüttel Stefanie Scheu Anne-Charlotte Antoni Wiebke Hansen Manuela Büettner Miriam J. Gardiasch Astrid M. Westendorf Florian Wirsdörfer Eva Pastille Marcel Dudda Stefanie B. Flohé |
author_sort |
Anna Smirnov |
title |
Sphingosine 1-Phosphate- and C-C Chemokine Receptor 2-Dependent Activation of CD4+ Plasmacytoid Dendritic Cells in the Bone Marrow Contributes to Signs of Sepsis-Induced Immunosuppression |
title_short |
Sphingosine 1-Phosphate- and C-C Chemokine Receptor 2-Dependent Activation of CD4+ Plasmacytoid Dendritic Cells in the Bone Marrow Contributes to Signs of Sepsis-Induced Immunosuppression |
title_full |
Sphingosine 1-Phosphate- and C-C Chemokine Receptor 2-Dependent Activation of CD4+ Plasmacytoid Dendritic Cells in the Bone Marrow Contributes to Signs of Sepsis-Induced Immunosuppression |
title_fullStr |
Sphingosine 1-Phosphate- and C-C Chemokine Receptor 2-Dependent Activation of CD4+ Plasmacytoid Dendritic Cells in the Bone Marrow Contributes to Signs of Sepsis-Induced Immunosuppression |
title_full_unstemmed |
Sphingosine 1-Phosphate- and C-C Chemokine Receptor 2-Dependent Activation of CD4+ Plasmacytoid Dendritic Cells in the Bone Marrow Contributes to Signs of Sepsis-Induced Immunosuppression |
title_sort |
sphingosine 1-phosphate- and c-c chemokine receptor 2-dependent activation of cd4+ plasmacytoid dendritic cells in the bone marrow contributes to signs of sepsis-induced immunosuppression |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2017-11-01 |
description |
Sepsis is the dysregulated response of the host to systemic, mostly bacterial infection, and is associated with an enhanced susceptibility to life-threatening opportunistic infections. During polymicrobial sepsis, dendritic cells (DCs) secrete enhanced levels of interleukin (IL) 10 due to an altered differentiation in the bone marrow and contribute to the development of immunosuppression. We investigated the origin of the altered DC differentiation using murine cecal ligation and puncture (CLP), a model for human polymicrobial sepsis. Bone marrow cells (BMC) were isolated after sham or CLP operation, the cellular composition was analyzed, and bone marrow-derived DCs (BMDCs) were generated in vitro. From 24 h on after CLP, BMC gave rise to BMDC that released enhanced levels of IL-10. In parallel, a population of CD11chiMHCII+CD4+ DCs expanded in the bone marrow in a MyD88-dependent manner. Prior depletion of the CD11chiMHCII+CD4+ DCs from BMC in vitro reversed the increased IL-10 secretion of subsequently differentiating BMDC. The expansion of the CD11chiMHCII+CD4+ DC population in the bone marrow after CLP required the function of sphingosine 1-phosphate receptors and C-C chemokine receptor (CCR) 2, the receptor for C-C chemokine ligand (CCL) 2, but was not associated with monocyte mobilization. CD11chiMHCII+CD4+ DCs were identified as plasmacytoid DCs (pDCs) that had acquired an activated phenotype according to their increased expression of MHC class II and CD86. A redistribution of CD4+ pDCs from MHC class II− to MHC class II+ cells concomitant with enhanced expression of CD11c finally led to the rise in the number of CD11chiMHCII+CD4+ DCs. Enhanced levels of CCL2 were found in the bone marrow of septic mice and the inhibition of CCR2 dampened the expression of CD86 on CD4+ pDCs after CLP in vitro. Depletion of pDCs reversed the bias of splenic DCs toward increased IL-10 synthesis after CLP in vivo. Thus, during polymicrobial sepsis, CD4+ pDCs are activated in the bone marrow and induce functional reprogramming of differentiating BMDC toward an immunosuppressive phenotype. |
topic |
dendritic cells bone marrow differentiation polymicrobial sepsis immunosuppression monocytes |
url |
http://journal.frontiersin.org/article/10.3389/fimmu.2017.01622/full |
work_keys_str_mv |
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doaj-f25aa22df371424487fd5f724ddd67b22020-11-24T21:46:02ZengFrontiers Media S.A.Frontiers in Immunology1664-32242017-11-01810.3389/fimmu.2017.01622286396Sphingosine 1-Phosphate- and C-C Chemokine Receptor 2-Dependent Activation of CD4+ Plasmacytoid Dendritic Cells in the Bone Marrow Contributes to Signs of Sepsis-Induced ImmunosuppressionAnna Smirnov0Stephanie Pohlmann1Melanie Nehring2Shafaqat Ali3Shafaqat Ali4Ritu Mann-Nüttel5Stefanie Scheu6Anne-Charlotte Antoni7Wiebke Hansen8Manuela Büettner9Miriam J. Gardiasch10Astrid M. Westendorf11Florian Wirsdörfer12Eva Pastille13Marcel Dudda14Stefanie B. Flohé15Department of Orthopedics and Trauma Surgery, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyDepartment of Orthopedics and Trauma Surgery, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyDepartment of Orthopedics and Trauma Surgery, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyInstitute of Medical Microbiology and Hospital Hygiene, University of Düsseldorf, Düsseldorf, GermanyCells in Motion, Cluster of Excellence, University of Münster, Münster, GermanyInstitute of Medical Microbiology and Hospital Hygiene, University of Düsseldorf, Düsseldorf, GermanyInstitute of Medical Microbiology and Hospital Hygiene, University of Düsseldorf, Düsseldorf, GermanyDepartment of Orthopedics and Trauma Surgery, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyInstitute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyInstitute of Functional and Applied Anatomy, Hannover Medical School, Hannover, GermanyDepartment of Orthopedics and Trauma Surgery, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyInstitute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyMedical Faculty, Institute of Cell Biology (Cancer Research), University of Duisburg-Essen, Essen, GermanyInstitute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyDepartment of Orthopedics and Trauma Surgery, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyDepartment of Orthopedics and Trauma Surgery, University Hospital Essen, University of Duisburg-Essen, Essen, GermanySepsis is the dysregulated response of the host to systemic, mostly bacterial infection, and is associated with an enhanced susceptibility to life-threatening opportunistic infections. During polymicrobial sepsis, dendritic cells (DCs) secrete enhanced levels of interleukin (IL) 10 due to an altered differentiation in the bone marrow and contribute to the development of immunosuppression. We investigated the origin of the altered DC differentiation using murine cecal ligation and puncture (CLP), a model for human polymicrobial sepsis. Bone marrow cells (BMC) were isolated after sham or CLP operation, the cellular composition was analyzed, and bone marrow-derived DCs (BMDCs) were generated in vitro. From 24 h on after CLP, BMC gave rise to BMDC that released enhanced levels of IL-10. In parallel, a population of CD11chiMHCII+CD4+ DCs expanded in the bone marrow in a MyD88-dependent manner. Prior depletion of the CD11chiMHCII+CD4+ DCs from BMC in vitro reversed the increased IL-10 secretion of subsequently differentiating BMDC. The expansion of the CD11chiMHCII+CD4+ DC population in the bone marrow after CLP required the function of sphingosine 1-phosphate receptors and C-C chemokine receptor (CCR) 2, the receptor for C-C chemokine ligand (CCL) 2, but was not associated with monocyte mobilization. CD11chiMHCII+CD4+ DCs were identified as plasmacytoid DCs (pDCs) that had acquired an activated phenotype according to their increased expression of MHC class II and CD86. A redistribution of CD4+ pDCs from MHC class II− to MHC class II+ cells concomitant with enhanced expression of CD11c finally led to the rise in the number of CD11chiMHCII+CD4+ DCs. Enhanced levels of CCL2 were found in the bone marrow of septic mice and the inhibition of CCR2 dampened the expression of CD86 on CD4+ pDCs after CLP in vitro. Depletion of pDCs reversed the bias of splenic DCs toward increased IL-10 synthesis after CLP in vivo. Thus, during polymicrobial sepsis, CD4+ pDCs are activated in the bone marrow and induce functional reprogramming of differentiating BMDC toward an immunosuppressive phenotype.http://journal.frontiersin.org/article/10.3389/fimmu.2017.01622/fulldendritic cellsbone marrowdifferentiationpolymicrobial sepsisimmunosuppressionmonocytes |