Development of Microsatellite Markers Derived from Expressed Sequence Tags of Polyporales for Genetic Diversity Analysis of Endangered Polyporus umbellatus
A large scale of EST sequences of Polyporales was screened in this investigation in order to identify EST-SSR markers for various applications. The distribution of EST sequences and SSRs in five families of Polyporales was analyzed, respectively. Mononucleotide was the most abundant type, followed b...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2015-01-01
|
Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2015/941357 |
id |
doaj-f2634a6af2314074877221b56a518c05 |
---|---|
record_format |
Article |
spelling |
doaj-f2634a6af2314074877221b56a518c052020-11-25T01:56:40ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/941357941357Development of Microsatellite Markers Derived from Expressed Sequence Tags of Polyporales for Genetic Diversity Analysis of Endangered Polyporus umbellatusYuejin Zhang0Yuanyuan Chen1Ruihong Wang2Ailin Zeng3Michael K. Deyholos4Jia Shu5Hongbo Guo6State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A&F University, Yangling 712100, ChinaDepartment of Biology, The University of British Columbia Okanagan, Kelowna, BC, V1V 1V7, CanadaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Sciences, Northwest A&F University, Yangling 712100, ChinaA large scale of EST sequences of Polyporales was screened in this investigation in order to identify EST-SSR markers for various applications. The distribution of EST sequences and SSRs in five families of Polyporales was analyzed, respectively. Mononucleotide was the most abundant type, followed by trinucleotide. Among five families, Ganodermataceae occupied the most SSR markers, followed by Coriolaceae. Functional prediction of SSR marker-containing EST sequences in Ganoderma lucidum obtained three main groups, namely, cellular component, biological process, and molecular function. Thirty EST-SSR primers were designed to evaluate the genetic diversity of 13 natural Polyporus umbellatus accessions. Twenty one EST-SSRs were polymorphic with average PIC value of 0.33 and transferability rate of 71%. These 13 P. umbellatus accessions showed relatively high genetic diversity. The expected heterozygosity, Nei’s gene diversity, and Shannon information index were 0.41, 0.39, and 0.57, respectively. Both UPGMA dendrogram and principal coordinate analysis (PCA) showed the same cluster result that divided the 13 accessions into three or four groups.http://dx.doi.org/10.1155/2015/941357 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuejin Zhang Yuanyuan Chen Ruihong Wang Ailin Zeng Michael K. Deyholos Jia Shu Hongbo Guo |
spellingShingle |
Yuejin Zhang Yuanyuan Chen Ruihong Wang Ailin Zeng Michael K. Deyholos Jia Shu Hongbo Guo Development of Microsatellite Markers Derived from Expressed Sequence Tags of Polyporales for Genetic Diversity Analysis of Endangered Polyporus umbellatus BioMed Research International |
author_facet |
Yuejin Zhang Yuanyuan Chen Ruihong Wang Ailin Zeng Michael K. Deyholos Jia Shu Hongbo Guo |
author_sort |
Yuejin Zhang |
title |
Development of Microsatellite Markers Derived from Expressed Sequence Tags of Polyporales for Genetic Diversity Analysis of Endangered Polyporus umbellatus |
title_short |
Development of Microsatellite Markers Derived from Expressed Sequence Tags of Polyporales for Genetic Diversity Analysis of Endangered Polyporus umbellatus |
title_full |
Development of Microsatellite Markers Derived from Expressed Sequence Tags of Polyporales for Genetic Diversity Analysis of Endangered Polyporus umbellatus |
title_fullStr |
Development of Microsatellite Markers Derived from Expressed Sequence Tags of Polyporales for Genetic Diversity Analysis of Endangered Polyporus umbellatus |
title_full_unstemmed |
Development of Microsatellite Markers Derived from Expressed Sequence Tags of Polyporales for Genetic Diversity Analysis of Endangered Polyporus umbellatus |
title_sort |
development of microsatellite markers derived from expressed sequence tags of polyporales for genetic diversity analysis of endangered polyporus umbellatus |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2015-01-01 |
description |
A large scale of EST sequences of Polyporales was screened in this investigation in order to identify EST-SSR markers for various applications. The distribution of EST sequences and SSRs in five families of Polyporales was analyzed, respectively. Mononucleotide was the most abundant type, followed by trinucleotide. Among five families, Ganodermataceae occupied the most SSR markers, followed by Coriolaceae. Functional prediction of SSR marker-containing EST sequences in Ganoderma lucidum obtained three main groups, namely, cellular component, biological process, and molecular function. Thirty EST-SSR primers were designed to evaluate the genetic diversity of 13 natural Polyporus umbellatus accessions. Twenty one EST-SSRs were polymorphic with average PIC value of 0.33 and transferability rate of 71%. These 13 P. umbellatus accessions showed relatively high genetic diversity. The expected heterozygosity, Nei’s gene diversity, and Shannon information index were 0.41, 0.39, and 0.57, respectively. Both UPGMA dendrogram and principal coordinate analysis (PCA) showed the same cluster result that divided the 13 accessions into three or four groups. |
url |
http://dx.doi.org/10.1155/2015/941357 |
work_keys_str_mv |
AT yuejinzhang developmentofmicrosatellitemarkersderivedfromexpressedsequencetagsofpolyporalesforgeneticdiversityanalysisofendangeredpolyporusumbellatus AT yuanyuanchen developmentofmicrosatellitemarkersderivedfromexpressedsequencetagsofpolyporalesforgeneticdiversityanalysisofendangeredpolyporusumbellatus AT ruihongwang developmentofmicrosatellitemarkersderivedfromexpressedsequencetagsofpolyporalesforgeneticdiversityanalysisofendangeredpolyporusumbellatus AT ailinzeng developmentofmicrosatellitemarkersderivedfromexpressedsequencetagsofpolyporalesforgeneticdiversityanalysisofendangeredpolyporusumbellatus AT michaelkdeyholos developmentofmicrosatellitemarkersderivedfromexpressedsequencetagsofpolyporalesforgeneticdiversityanalysisofendangeredpolyporusumbellatus AT jiashu developmentofmicrosatellitemarkersderivedfromexpressedsequencetagsofpolyporalesforgeneticdiversityanalysisofendangeredpolyporusumbellatus AT hongboguo developmentofmicrosatellitemarkersderivedfromexpressedsequencetagsofpolyporalesforgeneticdiversityanalysisofendangeredpolyporusumbellatus |
_version_ |
1724978717185277952 |