Transcriptome analysis of osmotic-responsive genes in ABA-dependent and -independent pathways in wheat (Triticum aestivum L.) roots
Bread wheat is one of the most important crops in the world. However, osmotic stress significantly inhibits wheat growth and development, and reduces crop yield and quality. Plants respond to osmotic stress mainly through abscisic acid (ABA)-dependent and -independent pathways. In this study, root t...
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doaj-f2712bd38a48494681ea08361fec0bee2020-11-25T00:24:51ZengPeerJ Inc.PeerJ2167-83592019-03-017e651910.7717/peerj.6519Transcriptome analysis of osmotic-responsive genes in ABA-dependent and -independent pathways in wheat (Triticum aestivum L.) rootsChunxi LiWenli ZhangMeng YuanLina JiangBo SunDaijing ZhangYun ShaoAnqi LiuXueqing LiuJianhui MaBread wheat is one of the most important crops in the world. However, osmotic stress significantly inhibits wheat growth and development, and reduces crop yield and quality. Plants respond to osmotic stress mainly through abscisic acid (ABA)-dependent and -independent pathways. In this study, root transcriptome profiles of wheat seedlings exposed to osmotic stress and exogenous ABA were analysed to identify osmotic-responsive genes belonging to the ABA-dependent or -independent pathways. We found that osmotic stress promoted proline biosynthesis in the ABA-dependent pathway, and trehalose biosynthesis is likely promoted among soluble sugars to maintain protein bioactivity under osmotic stress. In wheat roots subjected to osmotic stress, calcium ions, and glutathione exert their functions mainly through calcium-binding protein (CaM/CML) and glutathione-S-transferase, respectively, depending on both pathways. In addition, a complex relationship among phytohormones signal transduction was observed in response to osmotic stress. The findings of this study deepen our understanding of the molecular mechanisms of osmotic-stress resistance, and provide several candidate osmotic-responsive genes for further study.https://peerj.com/articles/6519.pdfTriticum aestivum L.Osmotic stressAbscisic acidTranscriptomic |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chunxi Li Wenli Zhang Meng Yuan Lina Jiang Bo Sun Daijing Zhang Yun Shao Anqi Liu Xueqing Liu Jianhui Ma |
spellingShingle |
Chunxi Li Wenli Zhang Meng Yuan Lina Jiang Bo Sun Daijing Zhang Yun Shao Anqi Liu Xueqing Liu Jianhui Ma Transcriptome analysis of osmotic-responsive genes in ABA-dependent and -independent pathways in wheat (Triticum aestivum L.) roots PeerJ Triticum aestivum L. Osmotic stress Abscisic acid Transcriptomic |
author_facet |
Chunxi Li Wenli Zhang Meng Yuan Lina Jiang Bo Sun Daijing Zhang Yun Shao Anqi Liu Xueqing Liu Jianhui Ma |
author_sort |
Chunxi Li |
title |
Transcriptome analysis of osmotic-responsive genes in ABA-dependent and -independent pathways in wheat (Triticum aestivum L.) roots |
title_short |
Transcriptome analysis of osmotic-responsive genes in ABA-dependent and -independent pathways in wheat (Triticum aestivum L.) roots |
title_full |
Transcriptome analysis of osmotic-responsive genes in ABA-dependent and -independent pathways in wheat (Triticum aestivum L.) roots |
title_fullStr |
Transcriptome analysis of osmotic-responsive genes in ABA-dependent and -independent pathways in wheat (Triticum aestivum L.) roots |
title_full_unstemmed |
Transcriptome analysis of osmotic-responsive genes in ABA-dependent and -independent pathways in wheat (Triticum aestivum L.) roots |
title_sort |
transcriptome analysis of osmotic-responsive genes in aba-dependent and -independent pathways in wheat (triticum aestivum l.) roots |
publisher |
PeerJ Inc. |
series |
PeerJ |
issn |
2167-8359 |
publishDate |
2019-03-01 |
description |
Bread wheat is one of the most important crops in the world. However, osmotic stress significantly inhibits wheat growth and development, and reduces crop yield and quality. Plants respond to osmotic stress mainly through abscisic acid (ABA)-dependent and -independent pathways. In this study, root transcriptome profiles of wheat seedlings exposed to osmotic stress and exogenous ABA were analysed to identify osmotic-responsive genes belonging to the ABA-dependent or -independent pathways. We found that osmotic stress promoted proline biosynthesis in the ABA-dependent pathway, and trehalose biosynthesis is likely promoted among soluble sugars to maintain protein bioactivity under osmotic stress. In wheat roots subjected to osmotic stress, calcium ions, and glutathione exert their functions mainly through calcium-binding protein (CaM/CML) and glutathione-S-transferase, respectively, depending on both pathways. In addition, a complex relationship among phytohormones signal transduction was observed in response to osmotic stress. The findings of this study deepen our understanding of the molecular mechanisms of osmotic-stress resistance, and provide several candidate osmotic-responsive genes for further study. |
topic |
Triticum aestivum L. Osmotic stress Abscisic acid Transcriptomic |
url |
https://peerj.com/articles/6519.pdf |
work_keys_str_mv |
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1725351241913991168 |