Tomatidine Improves Pulmonary Inflammation in Mice with Acute Lung Injury
Tomatidine, which is isolated from green tomato, can ameliorate inflammation and oxidative stress in cells and animal experiments and has been shown to improve airway inflammation in a murine model of asthma. Here, we investigated whether tomatidine can ameliorate acute lung injury in mice. Mice wer...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2021-01-01
|
Series: | Mediators of Inflammation |
Online Access: | http://dx.doi.org/10.1155/2021/4544294 |
id |
doaj-f27aacba9b2442a2b152d3f52b7fc9d1 |
---|---|
record_format |
Article |
spelling |
doaj-f27aacba9b2442a2b152d3f52b7fc9d12021-09-20T00:30:15ZengHindawi LimitedMediators of Inflammation1466-18612021-01-01202110.1155/2021/4544294Tomatidine Improves Pulmonary Inflammation in Mice with Acute Lung InjuryWen-Chung Huang0Shu-Ju Wu1Ya-Ling Chen2Chwan-Fwu Lin3Chian-Jiun Liou4Graduate Institute of Health Industry TechnologyDepartment of Nutrition and Health SciencesSchool of Nutrition and Health SciencesDepartment of Cosmetic ScienceDivision of AllergyTomatidine, which is isolated from green tomato, can ameliorate inflammation and oxidative stress in cells and animal experiments and has been shown to improve airway inflammation in a murine model of asthma. Here, we investigated whether tomatidine can ameliorate acute lung injury in mice. Mice were given tomatidine by intraperitoneal injection for 7 consecutive days, and then, lung injury was induced via intratracheal instillation of lipopolysaccharide (LPS). Tomatidine reduced inflammatory cytokine expressions in bronchoalveolar lavage fluid (BALF), attenuated neutrophil infiltration in the BALF and lung tissue, increased superoxide dismutase activity and glutathione levels, and alleviated myeloperoxidase expression in the lung tissue of mice with lung injury. Tomatidine also decreased inflammatory cytokine and chemokine gene expression in inflammatory lungs and attenuated the phosphorylation of mitogen-activated protein kinase and nuclear factor kappa B. Furthermore, tomatidine enhanced the production of heme oxygenase-1, decreased the secretion of inflammatory cytokines and chemokines in LPS-stimulated lung epithelial cells, and attenuated THP-1 monocyte adhesion. Our findings suggest that tomatidine attenuates oxidative stress and inflammation, improving acute lung injury in mice.http://dx.doi.org/10.1155/2021/4544294 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wen-Chung Huang Shu-Ju Wu Ya-Ling Chen Chwan-Fwu Lin Chian-Jiun Liou |
spellingShingle |
Wen-Chung Huang Shu-Ju Wu Ya-Ling Chen Chwan-Fwu Lin Chian-Jiun Liou Tomatidine Improves Pulmonary Inflammation in Mice with Acute Lung Injury Mediators of Inflammation |
author_facet |
Wen-Chung Huang Shu-Ju Wu Ya-Ling Chen Chwan-Fwu Lin Chian-Jiun Liou |
author_sort |
Wen-Chung Huang |
title |
Tomatidine Improves Pulmonary Inflammation in Mice with Acute Lung Injury |
title_short |
Tomatidine Improves Pulmonary Inflammation in Mice with Acute Lung Injury |
title_full |
Tomatidine Improves Pulmonary Inflammation in Mice with Acute Lung Injury |
title_fullStr |
Tomatidine Improves Pulmonary Inflammation in Mice with Acute Lung Injury |
title_full_unstemmed |
Tomatidine Improves Pulmonary Inflammation in Mice with Acute Lung Injury |
title_sort |
tomatidine improves pulmonary inflammation in mice with acute lung injury |
publisher |
Hindawi Limited |
series |
Mediators of Inflammation |
issn |
1466-1861 |
publishDate |
2021-01-01 |
description |
Tomatidine, which is isolated from green tomato, can ameliorate inflammation and oxidative stress in cells and animal experiments and has been shown to improve airway inflammation in a murine model of asthma. Here, we investigated whether tomatidine can ameliorate acute lung injury in mice. Mice were given tomatidine by intraperitoneal injection for 7 consecutive days, and then, lung injury was induced via intratracheal instillation of lipopolysaccharide (LPS). Tomatidine reduced inflammatory cytokine expressions in bronchoalveolar lavage fluid (BALF), attenuated neutrophil infiltration in the BALF and lung tissue, increased superoxide dismutase activity and glutathione levels, and alleviated myeloperoxidase expression in the lung tissue of mice with lung injury. Tomatidine also decreased inflammatory cytokine and chemokine gene expression in inflammatory lungs and attenuated the phosphorylation of mitogen-activated protein kinase and nuclear factor kappa B. Furthermore, tomatidine enhanced the production of heme oxygenase-1, decreased the secretion of inflammatory cytokines and chemokines in LPS-stimulated lung epithelial cells, and attenuated THP-1 monocyte adhesion. Our findings suggest that tomatidine attenuates oxidative stress and inflammation, improving acute lung injury in mice. |
url |
http://dx.doi.org/10.1155/2021/4544294 |
work_keys_str_mv |
AT wenchunghuang tomatidineimprovespulmonaryinflammationinmicewithacutelunginjury AT shujuwu tomatidineimprovespulmonaryinflammationinmicewithacutelunginjury AT yalingchen tomatidineimprovespulmonaryinflammationinmicewithacutelunginjury AT chwanfwulin tomatidineimprovespulmonaryinflammationinmicewithacutelunginjury AT chianjiunliou tomatidineimprovespulmonaryinflammationinmicewithacutelunginjury |
_version_ |
1717375100849750016 |