Function of Brassica napus BnABI3 in Arabidopsis gs1, an Allele of AtABI3, in Seed Development and Stress Response
Abscisic acid (ABA) has been implicated in plant adaptation to various environmental stresses in addition to the regulation of seed dormancy and leaf senescence. ABI3 is a B3 domain-containing family protein and functions in the ABA signaling pathway during seed development. To date, the ABI3 orthol...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2019-02-01
|
Series: | Frontiers in Plant Science |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.00067/full |
id |
doaj-f28bbb141a12442488220341bb0dae46 |
---|---|
record_format |
Article |
spelling |
doaj-f28bbb141a12442488220341bb0dae462020-11-24T21:05:42ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-02-011010.3389/fpls.2019.00067426804Function of Brassica napus BnABI3 in Arabidopsis gs1, an Allele of AtABI3, in Seed Development and Stress ResponsePeipei XuWeiming CaiAbscisic acid (ABA) has been implicated in plant adaptation to various environmental stresses in addition to the regulation of seed dormancy and leaf senescence. ABI3 is a B3 domain-containing family protein and functions in the ABA signaling pathway during seed development. To date, the ABI3 orthologous have not been studied in Brassica napus. The aim of this study is to investigate the function of BnABI3 in plant development and stress response. Here, we identified an Arabidopsis line (gs1) from a population of mutagenized seeds and showed that GS1 is a new allele of AtABI3. When the Arabidopsis gs1 mutant was transformed with the BnABI3 gene, the transformed plants produced seeds that turned yellow and acquired desiccation tolerance. Moreover, BnABI3 regulates seed coat development and mucilage secretion by directly targeting the AtMUM1 and AtGATL5 genes. In addition, we showed that BnABI3 expression rescued gs1 freezing-induced green seed coloration by targeting AtSGR1/2 in transgenic Arabidopsis. BnABI3 is also involved in lateral root development and conferred a novel interaction between ABA and auxin signaling in roots. The potential role of ABI3 protein in endoplasmic reticulum homoeostasis was also tested. Altogether, our results indicated that BnABI3 mediates both plant development and the stress response.https://www.frontiersin.org/article/10.3389/fpls.2019.00067/fullBrassica napusfunctional complementationfreezing tolerancedesiccation toleranceER stressroot growth |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Peipei Xu Weiming Cai |
spellingShingle |
Peipei Xu Weiming Cai Function of Brassica napus BnABI3 in Arabidopsis gs1, an Allele of AtABI3, in Seed Development and Stress Response Frontiers in Plant Science Brassica napus functional complementation freezing tolerance desiccation tolerance ER stress root growth |
author_facet |
Peipei Xu Weiming Cai |
author_sort |
Peipei Xu |
title |
Function of Brassica napus BnABI3 in Arabidopsis gs1, an Allele of AtABI3, in Seed Development and Stress Response |
title_short |
Function of Brassica napus BnABI3 in Arabidopsis gs1, an Allele of AtABI3, in Seed Development and Stress Response |
title_full |
Function of Brassica napus BnABI3 in Arabidopsis gs1, an Allele of AtABI3, in Seed Development and Stress Response |
title_fullStr |
Function of Brassica napus BnABI3 in Arabidopsis gs1, an Allele of AtABI3, in Seed Development and Stress Response |
title_full_unstemmed |
Function of Brassica napus BnABI3 in Arabidopsis gs1, an Allele of AtABI3, in Seed Development and Stress Response |
title_sort |
function of brassica napus bnabi3 in arabidopsis gs1, an allele of atabi3, in seed development and stress response |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2019-02-01 |
description |
Abscisic acid (ABA) has been implicated in plant adaptation to various environmental stresses in addition to the regulation of seed dormancy and leaf senescence. ABI3 is a B3 domain-containing family protein and functions in the ABA signaling pathway during seed development. To date, the ABI3 orthologous have not been studied in Brassica napus. The aim of this study is to investigate the function of BnABI3 in plant development and stress response. Here, we identified an Arabidopsis line (gs1) from a population of mutagenized seeds and showed that GS1 is a new allele of AtABI3. When the Arabidopsis gs1 mutant was transformed with the BnABI3 gene, the transformed plants produced seeds that turned yellow and acquired desiccation tolerance. Moreover, BnABI3 regulates seed coat development and mucilage secretion by directly targeting the AtMUM1 and AtGATL5 genes. In addition, we showed that BnABI3 expression rescued gs1 freezing-induced green seed coloration by targeting AtSGR1/2 in transgenic Arabidopsis. BnABI3 is also involved in lateral root development and conferred a novel interaction between ABA and auxin signaling in roots. The potential role of ABI3 protein in endoplasmic reticulum homoeostasis was also tested. Altogether, our results indicated that BnABI3 mediates both plant development and the stress response. |
topic |
Brassica napus functional complementation freezing tolerance desiccation tolerance ER stress root growth |
url |
https://www.frontiersin.org/article/10.3389/fpls.2019.00067/full |
work_keys_str_mv |
AT peipeixu functionofbrassicanapusbnabi3inarabidopsisgs1analleleofatabi3inseeddevelopmentandstressresponse AT weimingcai functionofbrassicanapusbnabi3inarabidopsisgs1analleleofatabi3inseeddevelopmentandstressresponse |
_version_ |
1716767765043347456 |