Microtube Array Membrane (MTAM)-Based Encapsulated Cell Therapy for Cancer Treatment
The treatment of cancer has evolved significantly in recent years with a strong focus on immunotherapy. Encapsulated Cell Therapy (ECT) for immunotherapy-based anti-cancer treatment is a unique niche within this landscape, where molecules such as signaling factors and antibodies produced from cells...
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doaj-f2a5934ae7cc46c7a5da56f0f3ba811e2020-11-25T03:54:56ZengMDPI AGMembranes2077-03752020-04-0110808010.3390/membranes10050080Microtube Array Membrane (MTAM)-Based Encapsulated Cell Therapy for Cancer TreatmentChee Ho Chew0Chih-Wei Lee1Wan-Ting Huang2Li-Wei Cheng3Amanda Chen4Tsai-Mu Cheng5Yen-Lin Liu6Chien-Chung Chen7Graduate Institute of Biomedical Materials & Tissue Engineering, Taipei Medical University, Xinyi District, Taipei 11031, TaiwanGraduate Institute of Biomedical Materials & Tissue Engineering, Taipei Medical University, Xinyi District, Taipei 11031, TaiwanGraduate Institute of Biomedical Materials & Tissue Engineering, Taipei Medical University, Xinyi District, Taipei 11031, TaiwanGraduate Institute of Biomedical Materials & Tissue Engineering, Taipei Medical University, Xinyi District, Taipei 11031, TaiwanDepartment of Biochemistry, University of Washington, Seattle, WA 98195, USAThe PhD Program for Translational Medicine, Taipei Medical University, Taipei 11052, TaiwanDepartment of Pediatrics, Taipei Medical University Hospital, Taipei 11052, TaiwanGraduate Institute of Biomedical Materials & Tissue Engineering, Taipei Medical University, Xinyi District, Taipei 11031, TaiwanThe treatment of cancer has evolved significantly in recent years with a strong focus on immunotherapy. Encapsulated Cell Therapy (ECT) for immunotherapy-based anti-cancer treatment is a unique niche within this landscape, where molecules such as signaling factors and antibodies produced from cells are encapsulated within a vehicle, with a host amount of benefits in terms of treatment efficacy and reduced side effects. However, traditional ECTs generally lie in two extremes; either a macro scale vehicle is utilized, resulting in a retrievable system but with limited diffusion and surface area, or a micro scale vehicle is utilized, resulting in a system that has excellent diffusion and surface area but is unretrievable in the event of side effects occurring, which greatly compromises the biosafety of patients. In this study we adapted our patented and novel electrospun Polysulfone (PSF) Microtube Array Membranes (MTAMs) as a ‘middle’ approach to the above dilemma, which possess excellent diffusion and surface area while being retrievable. Hybridoma cells were encapsulated within the PSF MTAMs, where they produced CEACAM6 antibodies to be used in the suppression of cancer cell line A549, MDA-MB-468 and PC 3 (control). <i>In vitro</i> and <i>in vivo</i> studies revealed excellent cell viability of hybridoma cells with continuous secretion of CEACAM6 antibodies which suppressed the MDA-MB-468 throughout the entire 21 days of experiment. Such outcome suggested that the PSF MTAMs were not only an excellent three-dimensional (3D) cell culture substrate but potentially also an excellent vehicle for the application in ECT systems. Future research needs to include a long term <i>in vivo</i> >6 months study before it can be used in clinical applications.https://www.mdpi.com/2077-0375/10/5/80encapsulated cell therapy (ECT)hybridomacancermicrotube array membrane (MTAM)electrospinning |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chee Ho Chew Chih-Wei Lee Wan-Ting Huang Li-Wei Cheng Amanda Chen Tsai-Mu Cheng Yen-Lin Liu Chien-Chung Chen |
spellingShingle |
Chee Ho Chew Chih-Wei Lee Wan-Ting Huang Li-Wei Cheng Amanda Chen Tsai-Mu Cheng Yen-Lin Liu Chien-Chung Chen Microtube Array Membrane (MTAM)-Based Encapsulated Cell Therapy for Cancer Treatment Membranes encapsulated cell therapy (ECT) hybridoma cancer microtube array membrane (MTAM) electrospinning |
author_facet |
Chee Ho Chew Chih-Wei Lee Wan-Ting Huang Li-Wei Cheng Amanda Chen Tsai-Mu Cheng Yen-Lin Liu Chien-Chung Chen |
author_sort |
Chee Ho Chew |
title |
Microtube Array Membrane (MTAM)-Based Encapsulated Cell Therapy for Cancer Treatment |
title_short |
Microtube Array Membrane (MTAM)-Based Encapsulated Cell Therapy for Cancer Treatment |
title_full |
Microtube Array Membrane (MTAM)-Based Encapsulated Cell Therapy for Cancer Treatment |
title_fullStr |
Microtube Array Membrane (MTAM)-Based Encapsulated Cell Therapy for Cancer Treatment |
title_full_unstemmed |
Microtube Array Membrane (MTAM)-Based Encapsulated Cell Therapy for Cancer Treatment |
title_sort |
microtube array membrane (mtam)-based encapsulated cell therapy for cancer treatment |
publisher |
MDPI AG |
series |
Membranes |
issn |
2077-0375 |
publishDate |
2020-04-01 |
description |
The treatment of cancer has evolved significantly in recent years with a strong focus on immunotherapy. Encapsulated Cell Therapy (ECT) for immunotherapy-based anti-cancer treatment is a unique niche within this landscape, where molecules such as signaling factors and antibodies produced from cells are encapsulated within a vehicle, with a host amount of benefits in terms of treatment efficacy and reduced side effects. However, traditional ECTs generally lie in two extremes; either a macro scale vehicle is utilized, resulting in a retrievable system but with limited diffusion and surface area, or a micro scale vehicle is utilized, resulting in a system that has excellent diffusion and surface area but is unretrievable in the event of side effects occurring, which greatly compromises the biosafety of patients. In this study we adapted our patented and novel electrospun Polysulfone (PSF) Microtube Array Membranes (MTAMs) as a ‘middle’ approach to the above dilemma, which possess excellent diffusion and surface area while being retrievable. Hybridoma cells were encapsulated within the PSF MTAMs, where they produced CEACAM6 antibodies to be used in the suppression of cancer cell line A549, MDA-MB-468 and PC 3 (control). <i>In vitro</i> and <i>in vivo</i> studies revealed excellent cell viability of hybridoma cells with continuous secretion of CEACAM6 antibodies which suppressed the MDA-MB-468 throughout the entire 21 days of experiment. Such outcome suggested that the PSF MTAMs were not only an excellent three-dimensional (3D) cell culture substrate but potentially also an excellent vehicle for the application in ECT systems. Future research needs to include a long term <i>in vivo</i> >6 months study before it can be used in clinical applications. |
topic |
encapsulated cell therapy (ECT) hybridoma cancer microtube array membrane (MTAM) electrospinning |
url |
https://www.mdpi.com/2077-0375/10/5/80 |
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