Live-cell p53 single-molecule binding is modulated by C-terminal acetylation and correlates with transcriptional activity

Live-cell p53 single-molecule binding is modulated by C-terminal acetylation and correlates with transcriptional activity

Both transcription binding kinetics and post-translational modifications of transcription factors are thought to play a role in the modulation of transcription. Here the authors use single-molecule tracking to directly demonstrate that p53 acetylation modulates promoter residence time and transcript...

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Main Authors: Alessia Loffreda, Emanuela Jacchetti, Sofia Antunes, Paolo Rainone, Tiziana Daniele, Tatsuya Morisaki, Marco E. Bianchi, Carlo Tacchetti, Davide Mazza
Format: Article
Language:English
Published: Nature Publishing Group 2017-08-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-017-00398-7
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spelling doaj-f2aea08fe9e3479693ecf280fdffb6692021-01-31T12:39:52ZengNature Publishing GroupNature Communications2041-17232017-08-018111210.1038/s41467-017-00398-7Live-cell p53 single-molecule binding is modulated by C-terminal acetylation and correlates with transcriptional activityAlessia Loffreda0Emanuela Jacchetti1Sofia Antunes2Paolo Rainone3Tiziana Daniele4Tatsuya Morisaki5Marco E. Bianchi6Carlo Tacchetti7Davide Mazza8Istituto Scientifico Ospedale San Raffaele, Centro di Imaging SperimentaleIstituto Scientifico Ospedale San Raffaele, Centro di Imaging SperimentaleIstituto Scientifico Ospedale San Raffaele, Centro di Imaging SperimentaleIstituto Scientifico Ospedale San Raffaele, Centro di Imaging SperimentaleIstituto Scientifico Ospedale San Raffaele, Centro di Imaging SperimentaleFluorescence Imaging Group, National Cancer Institute, NIHIstituto Scientifico Ospedale San Raffaele, Chromatin Dynamics UnitIstituto Scientifico Ospedale San Raffaele, Centro di Imaging SperimentaleIstituto Scientifico Ospedale San Raffaele, Centro di Imaging SperimentaleBoth transcription binding kinetics and post-translational modifications of transcription factors are thought to play a role in the modulation of transcription. Here the authors use single-molecule tracking to directly demonstrate that p53 acetylation modulates promoter residence time and transcriptional activity.https://doi.org/10.1038/s41467-017-00398-7
collection DOAJ
language English
format Article
sources DOAJ
author Alessia Loffreda
Emanuela Jacchetti
Sofia Antunes
Paolo Rainone
Tiziana Daniele
Tatsuya Morisaki
Marco E. Bianchi
Carlo Tacchetti
Davide Mazza
spellingShingle Alessia Loffreda
Emanuela Jacchetti
Sofia Antunes
Paolo Rainone
Tiziana Daniele
Tatsuya Morisaki
Marco E. Bianchi
Carlo Tacchetti
Davide Mazza
Live-cell p53 single-molecule binding is modulated by C-terminal acetylation and correlates with transcriptional activity
Nature Communications
author_facet Alessia Loffreda
Emanuela Jacchetti
Sofia Antunes
Paolo Rainone
Tiziana Daniele
Tatsuya Morisaki
Marco E. Bianchi
Carlo Tacchetti
Davide Mazza
author_sort Alessia Loffreda
title Live-cell p53 single-molecule binding is modulated by C-terminal acetylation and correlates with transcriptional activity
title_short Live-cell p53 single-molecule binding is modulated by C-terminal acetylation and correlates with transcriptional activity
title_full Live-cell p53 single-molecule binding is modulated by C-terminal acetylation and correlates with transcriptional activity
title_fullStr Live-cell p53 single-molecule binding is modulated by C-terminal acetylation and correlates with transcriptional activity
title_full_unstemmed Live-cell p53 single-molecule binding is modulated by C-terminal acetylation and correlates with transcriptional activity
title_sort live-cell p53 single-molecule binding is modulated by c-terminal acetylation and correlates with transcriptional activity
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2017-08-01
description Both transcription binding kinetics and post-translational modifications of transcription factors are thought to play a role in the modulation of transcription. Here the authors use single-molecule tracking to directly demonstrate that p53 acetylation modulates promoter residence time and transcriptional activity.
url https://doi.org/10.1038/s41467-017-00398-7
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