Structural basis of interaction between mesenchymal stem cells and decellularized rat heart matrix

• Main Authors: A. S. Sotnichenko, A. A. Slavinsky, I. V. Gilevich, E. V. Kuevda, I. S. Gumenyuk, E. A. Gubareva, P. . Macchiarini
• Format: Article
• Language: Russian
• Published: Ministry of Healthcare of the Russian Federation. “Kuban State Medical University” 2015-04-01
• Series: Кубанский научный медицинский вестник
• Subjects: децеллюляризация; рецеллюляризация; сердце; стволовые клетки; тканевая инженерия; decellularization; recellularization; heart; stem cells; tissue engineering
• Online Access: https://ksma.elpub.ru/jour/article/view/314
• ISSN: 1608-6228; 2541-9544

The study presents data about structural interaction between decellularizedrat heart matrix and stem cells used for recellularization. The effect of decellularized scaffold influence on the potential cells ability for differentiation without growth factors was evaluated. Morphological analysis of recellularized rat heart matrix was conducted. Rat heart scaffold influence on the proliferative cells capacity during recellularization process was assessed by immunohistochemical analysis. Cell viability on the scaffold was determined by the colorimetric test with MTT-reagent.