The Neuroprotective Effect of Hemin and the Related Mechanism in Sevoflurane Exposed Neonatal Rats
BackgroundMany studies have reported that sevoflurane can increase neuronal apoptosis and result in cognitive deficits in rodents. Although neurotoxicity may be associated with mitochondrial dysfunction and oxidative stress, the exact mechanism remains unclear. In order to evaluate potential treatme...
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doaj-f2b7c0e76f394a91aaca82c4f25a135e2020-11-25T01:58:50ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2019-05-011310.3389/fnins.2019.00537441281The Neuroprotective Effect of Hemin and the Related Mechanism in Sevoflurane Exposed Neonatal RatsFan Yang0Yangyang Shan1Zhiyin Tang2Xiuying Wu3Congjie Bi4Yongfang Zhang5Yan Gao6Hongtao LiuDepartment of Anesthesiology, Shengjing Hospital, China Medical University, Shenyang, ChinaDepartment of Anesthesiology, Shengjing Hospital, China Medical University, Shenyang, ChinaDepartment of Anesthesiology, Shengjing Hospital, China Medical University, Shenyang, ChinaDepartment of Anesthesiology, Shengjing Hospital, China Medical University, Shenyang, ChinaDepartment of Anesthesiology, Dalian Central Hospital, Dalian, ChinaDepartment of Anesthesiology, Shengjing Hospital, China Medical University, Shenyang, ChinaDepartment of Anesthesiology, The First Affiliated Hospital of Hebei North University, Zhangjiakou, ChinaBackgroundMany studies have reported that sevoflurane can increase neuronal apoptosis and result in cognitive deficits in rodents. Although neurotoxicity may be associated with mitochondrial dysfunction and oxidative stress, the exact mechanism remains unclear. In order to evaluate potential treatment therapies, we studied the effects of hemin on neurotoxicity of neonatal rat sevoflurane exposure.MethodsPostnatal day (P) seven rats were assigned randomly to four groups; (1) group C: non-anesthesia, (2) group H: intraperitoneal hemin (50 mg kg−1) treatment on days 5 and 6, (3) group S: 3% sevoflurane exposure for 4 h, and (4) group SH: hemin treatment + sevoflurane exposure. The expression of neuroglobin in neonatal hippocampus was determined by western blot and immunohistochemistry. Neuroglobin was localized by immunofluorescence. Western blot for the expression of cleaved caspase-3 and TUNEL were used to detect neonatal hippocampal apoptosis, and cytochrome c was used to evaluate mitochondrial function. Drp-1 and Mfn-2 immunoblotting were used to assess mitochondrial dynamics. The Morris water maze test was performed to detect cognitive function in the rats on P30.ResultsExposure to sevoflurane increased the expression of cleaved caspase-3, cytochrome c, and Drp1 in the neonatal hippocampus and resulted in cognitive deficiency but decreased expression of Mfn2. Hemin reduced apoptosis, improved mitochondrial dynamics and ameliorated the cognitive impairment caused by sevoflurane exposure.ConclusionHemin reduced neuronal apoptosis, improved mitochondrial dynamics and protected against cognitive deficits induced by sevoflurane in neonatal rats. This neuroprotective effect may be achieved by increasing the expression of neuroglobin.https://www.frontiersin.org/article/10.3389/fnins.2019.00537/fullheminmitochondrianeuroglobinneurotoxicitysevoflurane |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fan Yang Yangyang Shan Zhiyin Tang Xiuying Wu Congjie Bi Yongfang Zhang Yan Gao Hongtao Liu |
spellingShingle |
Fan Yang Yangyang Shan Zhiyin Tang Xiuying Wu Congjie Bi Yongfang Zhang Yan Gao Hongtao Liu The Neuroprotective Effect of Hemin and the Related Mechanism in Sevoflurane Exposed Neonatal Rats Frontiers in Neuroscience hemin mitochondria neuroglobin neurotoxicity sevoflurane |
author_facet |
Fan Yang Yangyang Shan Zhiyin Tang Xiuying Wu Congjie Bi Yongfang Zhang Yan Gao Hongtao Liu |
author_sort |
Fan Yang |
title |
The Neuroprotective Effect of Hemin and the Related Mechanism in Sevoflurane Exposed Neonatal Rats |
title_short |
The Neuroprotective Effect of Hemin and the Related Mechanism in Sevoflurane Exposed Neonatal Rats |
title_full |
The Neuroprotective Effect of Hemin and the Related Mechanism in Sevoflurane Exposed Neonatal Rats |
title_fullStr |
The Neuroprotective Effect of Hemin and the Related Mechanism in Sevoflurane Exposed Neonatal Rats |
title_full_unstemmed |
The Neuroprotective Effect of Hemin and the Related Mechanism in Sevoflurane Exposed Neonatal Rats |
title_sort |
neuroprotective effect of hemin and the related mechanism in sevoflurane exposed neonatal rats |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Neuroscience |
issn |
1662-453X |
publishDate |
2019-05-01 |
description |
BackgroundMany studies have reported that sevoflurane can increase neuronal apoptosis and result in cognitive deficits in rodents. Although neurotoxicity may be associated with mitochondrial dysfunction and oxidative stress, the exact mechanism remains unclear. In order to evaluate potential treatment therapies, we studied the effects of hemin on neurotoxicity of neonatal rat sevoflurane exposure.MethodsPostnatal day (P) seven rats were assigned randomly to four groups; (1) group C: non-anesthesia, (2) group H: intraperitoneal hemin (50 mg kg−1) treatment on days 5 and 6, (3) group S: 3% sevoflurane exposure for 4 h, and (4) group SH: hemin treatment + sevoflurane exposure. The expression of neuroglobin in neonatal hippocampus was determined by western blot and immunohistochemistry. Neuroglobin was localized by immunofluorescence. Western blot for the expression of cleaved caspase-3 and TUNEL were used to detect neonatal hippocampal apoptosis, and cytochrome c was used to evaluate mitochondrial function. Drp-1 and Mfn-2 immunoblotting were used to assess mitochondrial dynamics. The Morris water maze test was performed to detect cognitive function in the rats on P30.ResultsExposure to sevoflurane increased the expression of cleaved caspase-3, cytochrome c, and Drp1 in the neonatal hippocampus and resulted in cognitive deficiency but decreased expression of Mfn2. Hemin reduced apoptosis, improved mitochondrial dynamics and ameliorated the cognitive impairment caused by sevoflurane exposure.ConclusionHemin reduced neuronal apoptosis, improved mitochondrial dynamics and protected against cognitive deficits induced by sevoflurane in neonatal rats. This neuroprotective effect may be achieved by increasing the expression of neuroglobin. |
topic |
hemin mitochondria neuroglobin neurotoxicity sevoflurane |
url |
https://www.frontiersin.org/article/10.3389/fnins.2019.00537/full |
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