Genetic variability in Brazilian populations of Biomphalaria straminea complex detected by simple sequence repeat anchored polymerase chain reaction amplification
Biomphalaria glabrata, B. tenagophila and B. straminea are intermediate hosts of Schistosoma mansoni, in Brazil. The latter is of epidemiological importance in the northwest of Brazil and, due to morphological similarities, has been grouped with B. intermedia and B. kuhniana in a complex named B. st...
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Instituto Oswaldo Cruz, Ministério da Saúde
2001-05-01
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doaj-f2cddfa2913f4665a7639997892f61262020-11-25T00:55:10ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.0074-02761678-80602001-05-0196453554410.1590/S0074-02762001000400016Genetic variability in Brazilian populations of Biomphalaria straminea complex detected by simple sequence repeat anchored polymerase chain reaction amplificationRoberta L CaldeiraTeofânia HDA VidigalAndrew JG SimpsonOmar S CarvalhoBiomphalaria glabrata, B. tenagophila and B. straminea are intermediate hosts of Schistosoma mansoni, in Brazil. The latter is of epidemiological importance in the northwest of Brazil and, due to morphological similarities, has been grouped with B. intermedia and B. kuhniana in a complex named B. straminea. In the current work, we have standardized the simple sequence repeat anchored polymerase chain reaction (SSR-PCR) technique, using the primers (CA)8RY and K7, to study the genetic variability of these species. The similarity level was calculated using the Dice coefficient and genetic distance using the Nei and Li coefficient. The trees were obtained by the UPGMA and neighbor-joining methods. We have observed that the most related individuals belong to the same species and locality and that individuals from different localities, but of the same species, present clear heterogeneity. The trees generated using both methods showed similar topologies. The SSR-PCR technique was shown to be very efficient in intrapopulational and intraspecific studies of the B. straminea complex snails.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000400016snailsPlanorbidaeBiomphalaria stramineaBiomphalaria kuhnianaBiomphalaria intermediaSSR anchored PCR amplificationgenetic variability |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Roberta L Caldeira Teofânia HDA Vidigal Andrew JG Simpson Omar S Carvalho |
spellingShingle |
Roberta L Caldeira Teofânia HDA Vidigal Andrew JG Simpson Omar S Carvalho Genetic variability in Brazilian populations of Biomphalaria straminea complex detected by simple sequence repeat anchored polymerase chain reaction amplification Memórias do Instituto Oswaldo Cruz. snails Planorbidae Biomphalaria straminea Biomphalaria kuhniana Biomphalaria intermedia SSR anchored PCR amplification genetic variability |
author_facet |
Roberta L Caldeira Teofânia HDA Vidigal Andrew JG Simpson Omar S Carvalho |
author_sort |
Roberta L Caldeira |
title |
Genetic variability in Brazilian populations of Biomphalaria straminea complex detected by simple sequence repeat anchored polymerase chain reaction amplification |
title_short |
Genetic variability in Brazilian populations of Biomphalaria straminea complex detected by simple sequence repeat anchored polymerase chain reaction amplification |
title_full |
Genetic variability in Brazilian populations of Biomphalaria straminea complex detected by simple sequence repeat anchored polymerase chain reaction amplification |
title_fullStr |
Genetic variability in Brazilian populations of Biomphalaria straminea complex detected by simple sequence repeat anchored polymerase chain reaction amplification |
title_full_unstemmed |
Genetic variability in Brazilian populations of Biomphalaria straminea complex detected by simple sequence repeat anchored polymerase chain reaction amplification |
title_sort |
genetic variability in brazilian populations of biomphalaria straminea complex detected by simple sequence repeat anchored polymerase chain reaction amplification |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
series |
Memórias do Instituto Oswaldo Cruz. |
issn |
0074-0276 1678-8060 |
publishDate |
2001-05-01 |
description |
Biomphalaria glabrata, B. tenagophila and B. straminea are intermediate hosts of Schistosoma mansoni, in Brazil. The latter is of epidemiological importance in the northwest of Brazil and, due to morphological similarities, has been grouped with B. intermedia and B. kuhniana in a complex named B. straminea. In the current work, we have standardized the simple sequence repeat anchored polymerase chain reaction (SSR-PCR) technique, using the primers (CA)8RY and K7, to study the genetic variability of these species. The similarity level was calculated using the Dice coefficient and genetic distance using the Nei and Li coefficient. The trees were obtained by the UPGMA and neighbor-joining methods. We have observed that the most related individuals belong to the same species and locality and that individuals from different localities, but of the same species, present clear heterogeneity. The trees generated using both methods showed similar topologies. The SSR-PCR technique was shown to be very efficient in intrapopulational and intraspecific studies of the B. straminea complex snails. |
topic |
snails Planorbidae Biomphalaria straminea Biomphalaria kuhniana Biomphalaria intermedia SSR anchored PCR amplification genetic variability |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000400016 |
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