An improved assay of gangliosides separated by thin-layer chromatography

There were no differences in the recoveries of ganglioside sialic acid from silica gel G thin-layer chromatograms when they were sprayed with resorcinol reagent varying in normality between 3 and 10. However, both the temperature at and the time for which the plates were heated after spraying did af...

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Bibliographic Details
Main Authors:	A J Yates, D Thompson
Format:	Article
Language:	English
Published:	Elsevier 1977-09-01
Series:	Journal of Lipid Research
Subjects:	ganglioside sialic acid glycolipid
Online Access:	http://www.sciencedirect.com/science/article/pii/S0022227520416086

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spelling	doaj-f2d233f0824d4432942b2cef2d049e2a2021-04-24T05:53:49ZengElsevierJournal of Lipid Research0022-22751977-09-01185660663An improved assay of gangliosides separated by thin-layer chromatographyA J Yates0D Thompson1Division of Neuropathology, Department of Pathology, The Ohio State University, Columbus OH 43210Division of Neuropathology, Department of Pathology, The Ohio State University, Columbus OH 43210There were no differences in the recoveries of ganglioside sialic acid from silica gel G thin-layer chromatograms when they were sprayed with resorcinol reagent varying in normality between 3 and 10. However, both the temperature at and the time for which the plates were heated after spraying did affect the recoveries, which can reach 100% when the plates are heated for 6 min at 135°C.http://www.sciencedirect.com/science/article/pii/S0022227520416086gangliosidesialic acidglycolipid
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	A J Yates D Thompson
spellingShingle	A J Yates D Thompson An improved assay of gangliosides separated by thin-layer chromatography Journal of Lipid Research ganglioside sialic acid glycolipid
author_facet	A J Yates D Thompson
author_sort	A J Yates
title	An improved assay of gangliosides separated by thin-layer chromatography
title_short	An improved assay of gangliosides separated by thin-layer chromatography
title_full	An improved assay of gangliosides separated by thin-layer chromatography
title_fullStr	An improved assay of gangliosides separated by thin-layer chromatography
title_full_unstemmed	An improved assay of gangliosides separated by thin-layer chromatography
title_sort	improved assay of gangliosides separated by thin-layer chromatography
publisher	Elsevier
series	Journal of Lipid Research
issn	0022-2275
publishDate	1977-09-01
description	There were no differences in the recoveries of ganglioside sialic acid from silica gel G thin-layer chromatograms when they were sprayed with resorcinol reagent varying in normality between 3 and 10. However, both the temperature at and the time for which the plates were heated after spraying did affect the recoveries, which can reach 100% when the plates are heated for 6 min at 135°C.
topic	ganglioside sialic acid glycolipid
url	http://www.sciencedirect.com/science/article/pii/S0022227520416086
work_keys_str_mv	AT ajyates animprovedassayofgangliosidesseparatedbythinlayerchromatography AT dthompson animprovedassayofgangliosidesseparatedbythinlayerchromatography AT ajyates improvedassayofgangliosidesseparatedbythinlayerchromatography AT dthompson improvedassayofgangliosidesseparatedbythinlayerchromatography
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An improved assay of gangliosides separated by thin-layer chromatography

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