Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin?

Lifespan in poikilothermic organisms, such as Caenorhabditis elegans, can be substantially increased simply by decreasing growth temperature. To gain insights into the mechanistic underpinnings of this effect, we investigated the effects of temperature in development and adulthood on C. elegans life...

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Main Authors:	Dylan Henderson, Christian Huebner, Moses Markowitz, Nicole Taube, Zachary M. Harvanek, Ursula Jakob, Daniela Knoefler
Format:	Article
Language:	English
Published:	Elsevier 2018-04-01
Series:	Redox Biology
Online Access:	http://www.sciencedirect.com/science/article/pii/S2213231717306638

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spelling	doaj-f2d98732c6ab4aee9d70220b695960152020-11-25T02:40:11ZengElsevierRedox Biology2213-23172018-04-0114386390Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin?Dylan Henderson0Christian Huebner1Moses Markowitz2Nicole Taube3Zachary M. Harvanek4Ursula Jakob5Daniela Knoefler6Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USADepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USADepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USADepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USADepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USACorrespondence to: Department of Molecular, Cellular, and Developmental Biology, Natural Science Building, Room 4041, 830 N. University, Ann Arbor, MI 48109, USA.; Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USACorrespondence to: Department of Molecular, Cellular, and Developmental Biology, Natural Science Building, Room 4041, 830 N. University, Ann Arbor, MI 48109, USA.; Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USALifespan in poikilothermic organisms, such as Caenorhabditis elegans, can be substantially increased simply by decreasing growth temperature. To gain insights into the mechanistic underpinnings of this effect, we investigated the effects of temperature in development and adulthood on C. elegans lifespan. We found that worms exposed to 25 °C during development and shifted to 15 °C in adulthood exhibited an even longer lifespan than animals constantly kept at 15 °C. Analysis of the in vivo redox status demonstrated that at 25 °C, C. elegans larvae have a more reduced redox state and higher Prdx-2 expression levels than animals raised at 15 °C. Worms lacking prdx-2 fail to show the additional lifespan extension upon shift from 25 °C to 15 °C and reveal a lifespan similar to prdx-2 worms always kept at 15 °C. These results suggest that transiently altering the in vivo redox state during development can have highly beneficial long-term consequences for organisms. Keywords: Aging, Temperature, C. elegans, Oxidants, Peroxiredoxinhttp://www.sciencedirect.com/science/article/pii/S2213231717306638
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Dylan Henderson Christian Huebner Moses Markowitz Nicole Taube Zachary M. Harvanek Ursula Jakob Daniela Knoefler
spellingShingle	Dylan Henderson Christian Huebner Moses Markowitz Nicole Taube Zachary M. Harvanek Ursula Jakob Daniela Knoefler Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin? Redox Biology
author_facet	Dylan Henderson Christian Huebner Moses Markowitz Nicole Taube Zachary M. Harvanek Ursula Jakob Daniela Knoefler
author_sort	Dylan Henderson
title	Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin?
title_short	Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin?
title_full	Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin?
title_fullStr	Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin?
title_full_unstemmed	Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin?
title_sort	do developmental temperatures affect redox level and lifespan in c. elegans through upregulation of peroxiredoxin?
publisher	Elsevier
series	Redox Biology
issn	2213-2317
publishDate	2018-04-01
description	Lifespan in poikilothermic organisms, such as Caenorhabditis elegans, can be substantially increased simply by decreasing growth temperature. To gain insights into the mechanistic underpinnings of this effect, we investigated the effects of temperature in development and adulthood on C. elegans lifespan. We found that worms exposed to 25 °C during development and shifted to 15 °C in adulthood exhibited an even longer lifespan than animals constantly kept at 15 °C. Analysis of the in vivo redox status demonstrated that at 25 °C, C. elegans larvae have a more reduced redox state and higher Prdx-2 expression levels than animals raised at 15 °C. Worms lacking prdx-2 fail to show the additional lifespan extension upon shift from 25 °C to 15 °C and reveal a lifespan similar to prdx-2 worms always kept at 15 °C. These results suggest that transiently altering the in vivo redox state during development can have highly beneficial long-term consequences for organisms. Keywords: Aging, Temperature, C. elegans, Oxidants, Peroxiredoxin
url	http://www.sciencedirect.com/science/article/pii/S2213231717306638
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Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin?

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