Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin?
Lifespan in poikilothermic organisms, such as Caenorhabditis elegans, can be substantially increased simply by decreasing growth temperature. To gain insights into the mechanistic underpinnings of this effect, we investigated the effects of temperature in development and adulthood on C. elegans life...
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doaj-f2d98732c6ab4aee9d70220b695960152020-11-25T02:40:11ZengElsevierRedox Biology2213-23172018-04-0114386390Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin?Dylan Henderson0Christian Huebner1Moses Markowitz2Nicole Taube3Zachary M. Harvanek4Ursula Jakob5Daniela Knoefler6Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USADepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USADepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USADepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USADepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USACorrespondence to: Department of Molecular, Cellular, and Developmental Biology, Natural Science Building, Room 4041, 830 N. University, Ann Arbor, MI 48109, USA.; Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USACorrespondence to: Department of Molecular, Cellular, and Developmental Biology, Natural Science Building, Room 4041, 830 N. University, Ann Arbor, MI 48109, USA.; Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USALifespan in poikilothermic organisms, such as Caenorhabditis elegans, can be substantially increased simply by decreasing growth temperature. To gain insights into the mechanistic underpinnings of this effect, we investigated the effects of temperature in development and adulthood on C. elegans lifespan. We found that worms exposed to 25 °C during development and shifted to 15 °C in adulthood exhibited an even longer lifespan than animals constantly kept at 15 °C. Analysis of the in vivo redox status demonstrated that at 25 °C, C. elegans larvae have a more reduced redox state and higher Prdx-2 expression levels than animals raised at 15 °C. Worms lacking prdx-2 fail to show the additional lifespan extension upon shift from 25 °C to 15 °C and reveal a lifespan similar to prdx-2 worms always kept at 15 °C. These results suggest that transiently altering the in vivo redox state during development can have highly beneficial long-term consequences for organisms. Keywords: Aging, Temperature, C. elegans, Oxidants, Peroxiredoxinhttp://www.sciencedirect.com/science/article/pii/S2213231717306638 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dylan Henderson Christian Huebner Moses Markowitz Nicole Taube Zachary M. Harvanek Ursula Jakob Daniela Knoefler |
spellingShingle |
Dylan Henderson Christian Huebner Moses Markowitz Nicole Taube Zachary M. Harvanek Ursula Jakob Daniela Knoefler Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin? Redox Biology |
author_facet |
Dylan Henderson Christian Huebner Moses Markowitz Nicole Taube Zachary M. Harvanek Ursula Jakob Daniela Knoefler |
author_sort |
Dylan Henderson |
title |
Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin? |
title_short |
Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin? |
title_full |
Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin? |
title_fullStr |
Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin? |
title_full_unstemmed |
Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin? |
title_sort |
do developmental temperatures affect redox level and lifespan in c. elegans through upregulation of peroxiredoxin? |
publisher |
Elsevier |
series |
Redox Biology |
issn |
2213-2317 |
publishDate |
2018-04-01 |
description |
Lifespan in poikilothermic organisms, such as Caenorhabditis elegans, can be substantially increased simply by decreasing growth temperature. To gain insights into the mechanistic underpinnings of this effect, we investigated the effects of temperature in development and adulthood on C. elegans lifespan. We found that worms exposed to 25 °C during development and shifted to 15 °C in adulthood exhibited an even longer lifespan than animals constantly kept at 15 °C. Analysis of the in vivo redox status demonstrated that at 25 °C, C. elegans larvae have a more reduced redox state and higher Prdx-2 expression levels than animals raised at 15 °C. Worms lacking prdx-2 fail to show the additional lifespan extension upon shift from 25 °C to 15 °C and reveal a lifespan similar to prdx-2 worms always kept at 15 °C. These results suggest that transiently altering the in vivo redox state during development can have highly beneficial long-term consequences for organisms. Keywords: Aging, Temperature, C. elegans, Oxidants, Peroxiredoxin |
url |
http://www.sciencedirect.com/science/article/pii/S2213231717306638 |
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