Proteomic analysis reveals response of differential wheat (Triticum aestivum L.) genotypes to oxygen deficiency stress
Abstract Background Waterlogging is one of the main abiotic stresses that limit wheat production. Quantitative proteomics analysis has been applied in the study of crop abiotic stress as an effective way in recent years (e.g. salt stress, drought stress, heat stress and waterlogging stress). However...
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doaj-f2e80f1a8588442fb2d9899f009183362020-11-24T23:51:55ZengBMCBMC Genomics1471-21642019-01-0120111310.1186/s12864-018-5405-3Proteomic analysis reveals response of differential wheat (Triticum aestivum L.) genotypes to oxygen deficiency stressRui Pan0Dongli He1Le Xu2Meixue Zhou3Chengdao Li4Chu Wu5Yanhao Xu6Wenying Zhang7Hubei Collaborative Innovation Center for Grain Industry/ School of Agriculture, Yangtze UniversityCollege of Life Sciences, Hubei UniversityHubei Collaborative Innovation Center for Grain Industry/ School of Agriculture, Yangtze UniversityHubei Collaborative Innovation Center for Grain Industry/ School of Agriculture, Yangtze UniversityHubei Collaborative Innovation Center for Grain Industry/ School of Agriculture, Yangtze UniversityCollege of Horticulture and Gardening, Yangtze UniversityHubei Collaborative Innovation Center for Grain Industry/ School of Agriculture, Yangtze UniversityHubei Collaborative Innovation Center for Grain Industry/ School of Agriculture, Yangtze UniversityAbstract Background Waterlogging is one of the main abiotic stresses that limit wheat production. Quantitative proteomics analysis has been applied in the study of crop abiotic stress as an effective way in recent years (e.g. salt stress, drought stress, heat stress and waterlogging stress). However, only a few proteins related to primary metabolism and signal transduction, such as UDP - glucose dehydrogenase, UGP, beta glucosidases, were reported to response to waterlogging stress in wheat. The differentially expressed proteins between genotypes of wheat in response to waterlogging are less-defined. In this study, two wheat genotypes, one is sensitive to waterlogging stress (Seri M82, named as S) and the other is tolerant to waterlogging (CIGM90.863, named as T), were compared in seedling roots under hypoxia conditions to evaluate the different responses at proteomic level. Results A total of 4560 proteins were identified and the number of differentially expressed proteins (DEPs) were 361, 640, 788 in S and 33, 207, 279 in T in 1, 2, 3 days, respectively. These DEPs included 270 common proteins, 681 S-specific and 50 T-specific proteins, most of which were misc., protein processing, DNA and RNA processing, amino acid metabolism and stress related proteins induced by hypoxia. Some specific proteins related to waterlogging stress, including acid phosphatase, oxidant protective enzyme, S-adenosylmethionine synthetase 1, were significantly different between S and T. A total of 20 representative genes encoding DEPs, including 7 shared DEPs and 13 cultivar-specific DEPs, were selected for further RT-qPCR analysis. Fourteen genes showed consistent dynamic expression patterns at mRNA and protein levels. Conclusions Proteins involved in primary metabolisms and protein processing were inclined to be affected under hypoxia stress. The negative effects were more severe in the sensitive genotype. The expression patterns of some specific proteins, such as alcohol dehydrogenases and S-adenosylmethionine synthetase 1, could be applied as indexes for improving the waterlogging tolerance in wheat. Some specific proteins identified in this study will facilitate the subsequent protein function validation and biomarker development.http://link.springer.com/article/10.1186/s12864-018-5405-3Triticum aestivum L.Hypoxic stressWaterlogging toleranceProteomics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rui Pan Dongli He Le Xu Meixue Zhou Chengdao Li Chu Wu Yanhao Xu Wenying Zhang |
spellingShingle |
Rui Pan Dongli He Le Xu Meixue Zhou Chengdao Li Chu Wu Yanhao Xu Wenying Zhang Proteomic analysis reveals response of differential wheat (Triticum aestivum L.) genotypes to oxygen deficiency stress BMC Genomics Triticum aestivum L. Hypoxic stress Waterlogging tolerance Proteomics |
author_facet |
Rui Pan Dongli He Le Xu Meixue Zhou Chengdao Li Chu Wu Yanhao Xu Wenying Zhang |
author_sort |
Rui Pan |
title |
Proteomic analysis reveals response of differential wheat (Triticum aestivum L.) genotypes to oxygen deficiency stress |
title_short |
Proteomic analysis reveals response of differential wheat (Triticum aestivum L.) genotypes to oxygen deficiency stress |
title_full |
Proteomic analysis reveals response of differential wheat (Triticum aestivum L.) genotypes to oxygen deficiency stress |
title_fullStr |
Proteomic analysis reveals response of differential wheat (Triticum aestivum L.) genotypes to oxygen deficiency stress |
title_full_unstemmed |
Proteomic analysis reveals response of differential wheat (Triticum aestivum L.) genotypes to oxygen deficiency stress |
title_sort |
proteomic analysis reveals response of differential wheat (triticum aestivum l.) genotypes to oxygen deficiency stress |
publisher |
BMC |
series |
BMC Genomics |
issn |
1471-2164 |
publishDate |
2019-01-01 |
description |
Abstract Background Waterlogging is one of the main abiotic stresses that limit wheat production. Quantitative proteomics analysis has been applied in the study of crop abiotic stress as an effective way in recent years (e.g. salt stress, drought stress, heat stress and waterlogging stress). However, only a few proteins related to primary metabolism and signal transduction, such as UDP - glucose dehydrogenase, UGP, beta glucosidases, were reported to response to waterlogging stress in wheat. The differentially expressed proteins between genotypes of wheat in response to waterlogging are less-defined. In this study, two wheat genotypes, one is sensitive to waterlogging stress (Seri M82, named as S) and the other is tolerant to waterlogging (CIGM90.863, named as T), were compared in seedling roots under hypoxia conditions to evaluate the different responses at proteomic level. Results A total of 4560 proteins were identified and the number of differentially expressed proteins (DEPs) were 361, 640, 788 in S and 33, 207, 279 in T in 1, 2, 3 days, respectively. These DEPs included 270 common proteins, 681 S-specific and 50 T-specific proteins, most of which were misc., protein processing, DNA and RNA processing, amino acid metabolism and stress related proteins induced by hypoxia. Some specific proteins related to waterlogging stress, including acid phosphatase, oxidant protective enzyme, S-adenosylmethionine synthetase 1, were significantly different between S and T. A total of 20 representative genes encoding DEPs, including 7 shared DEPs and 13 cultivar-specific DEPs, were selected for further RT-qPCR analysis. Fourteen genes showed consistent dynamic expression patterns at mRNA and protein levels. Conclusions Proteins involved in primary metabolisms and protein processing were inclined to be affected under hypoxia stress. The negative effects were more severe in the sensitive genotype. The expression patterns of some specific proteins, such as alcohol dehydrogenases and S-adenosylmethionine synthetase 1, could be applied as indexes for improving the waterlogging tolerance in wheat. Some specific proteins identified in this study will facilitate the subsequent protein function validation and biomarker development. |
topic |
Triticum aestivum L. Hypoxic stress Waterlogging tolerance Proteomics |
url |
http://link.springer.com/article/10.1186/s12864-018-5405-3 |
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