Keratinocyte Growth Factor-1 Protects Radioiodine-Induced Salivary Gland Dysfunction in Mice
Background: Most patients with thyroid cancer suffer from salivary gland (SG) dysfunctions after radioiodine (RI) therapy. We investigated the effects of keratinocyte growth factor (KGF)-1 on RI-induced SG dysfunction in an animal model. Methods: Six C57BL/6 mice were assigned to each of the followi...
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doaj-f303fed5853f486d91e29e6f195f36172020-11-25T03:49:57ZengMDPI AGInternational Journal of Environmental Research and Public Health1661-78271660-46012020-08-01176322632210.3390/ijerph17176322Keratinocyte Growth Factor-1 Protects Radioiodine-Induced Salivary Gland Dysfunction in MiceJeong Mi Kim0Mi Eun Choi1Seok-Ki Kim2Ji Won Kim3Young-Mo Kim4Jeong-Seok Choi5Translational Research Center, Inha University, Incheon 22332, KoreaTranslational Research Center, Inha University, Incheon 22332, KoreaDepartment of Nuclear Medicine, National Cancer Center, Goyang 10408, KoreaTranslational Research Center, Inha University, Incheon 22332, KoreaTranslational Research Center, Inha University, Incheon 22332, KoreaTranslational Research Center, Inha University, Incheon 22332, KoreaBackground: Most patients with thyroid cancer suffer from salivary gland (SG) dysfunctions after radioiodine (RI) therapy. We investigated the effects of keratinocyte growth factor (KGF)-1 on RI-induced SG dysfunction in an animal model. Methods: Six C57BL/6 mice were assigned to each of the following groups: treatment naïve control group, RI group, and RI+KGF-1 group. Body and SG weights, salivary flow rates, salivary lag times and changes in 99mTc pertechnetate uptake and excretion were measured, and histologic changes were noted. Amylase activities and epidermal growth factor (EGF) concentrations in saliva were also measured. In addition, TUNEL assays were performed and apoptosis-related protein expressions were assessed. Results: RI-induced reductions in salivary flow rates and increases in salivary lag times observed in the RI group were not observed in RI+KGF-1 group. Mice in RI group had higher HIF1a levels than controls, but HIF1a levels in RI+KGF-1 group were similar to those in control group. Furthermore, mice in RI+KGF-1 group had more mucin stained acini and decreased periductal fibrosis than mice in RI group, and tissue remodeling of many salivary epithelial cells (AQP5) and endothelial cells (CD31) were observed in RI+KGF-1 group. Amylase activity and expression in saliva were greater in RI+KGF-1 group than in RI group, and fewer apoptotic cells were observed in RI+KGF-1 group. Furthermore, BCLxl (anti-apoptotic) expression was higher, and Bax (pro-apoptotic) expression was lower in RI+KGF-1 group than in RI group. Conclusions: Local delivery of KGF-1 might prevent RI-induced SG damage by reducing apoptosis.https://www.mdpi.com/1660-4601/17/17/6322radioiodinesalivary glandkeratinocyte growth factoranti-apoptosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jeong Mi Kim Mi Eun Choi Seok-Ki Kim Ji Won Kim Young-Mo Kim Jeong-Seok Choi |
spellingShingle |
Jeong Mi Kim Mi Eun Choi Seok-Ki Kim Ji Won Kim Young-Mo Kim Jeong-Seok Choi Keratinocyte Growth Factor-1 Protects Radioiodine-Induced Salivary Gland Dysfunction in Mice International Journal of Environmental Research and Public Health radioiodine salivary gland keratinocyte growth factor anti-apoptosis |
author_facet |
Jeong Mi Kim Mi Eun Choi Seok-Ki Kim Ji Won Kim Young-Mo Kim Jeong-Seok Choi |
author_sort |
Jeong Mi Kim |
title |
Keratinocyte Growth Factor-1 Protects Radioiodine-Induced Salivary Gland Dysfunction in Mice |
title_short |
Keratinocyte Growth Factor-1 Protects Radioiodine-Induced Salivary Gland Dysfunction in Mice |
title_full |
Keratinocyte Growth Factor-1 Protects Radioiodine-Induced Salivary Gland Dysfunction in Mice |
title_fullStr |
Keratinocyte Growth Factor-1 Protects Radioiodine-Induced Salivary Gland Dysfunction in Mice |
title_full_unstemmed |
Keratinocyte Growth Factor-1 Protects Radioiodine-Induced Salivary Gland Dysfunction in Mice |
title_sort |
keratinocyte growth factor-1 protects radioiodine-induced salivary gland dysfunction in mice |
publisher |
MDPI AG |
series |
International Journal of Environmental Research and Public Health |
issn |
1661-7827 1660-4601 |
publishDate |
2020-08-01 |
description |
Background: Most patients with thyroid cancer suffer from salivary gland (SG) dysfunctions after radioiodine (RI) therapy. We investigated the effects of keratinocyte growth factor (KGF)-1 on RI-induced SG dysfunction in an animal model. Methods: Six C57BL/6 mice were assigned to each of the following groups: treatment naïve control group, RI group, and RI+KGF-1 group. Body and SG weights, salivary flow rates, salivary lag times and changes in 99mTc pertechnetate uptake and excretion were measured, and histologic changes were noted. Amylase activities and epidermal growth factor (EGF) concentrations in saliva were also measured. In addition, TUNEL assays were performed and apoptosis-related protein expressions were assessed. Results: RI-induced reductions in salivary flow rates and increases in salivary lag times observed in the RI group were not observed in RI+KGF-1 group. Mice in RI group had higher HIF1a levels than controls, but HIF1a levels in RI+KGF-1 group were similar to those in control group. Furthermore, mice in RI+KGF-1 group had more mucin stained acini and decreased periductal fibrosis than mice in RI group, and tissue remodeling of many salivary epithelial cells (AQP5) and endothelial cells (CD31) were observed in RI+KGF-1 group. Amylase activity and expression in saliva were greater in RI+KGF-1 group than in RI group, and fewer apoptotic cells were observed in RI+KGF-1 group. Furthermore, BCLxl (anti-apoptotic) expression was higher, and Bax (pro-apoptotic) expression was lower in RI+KGF-1 group than in RI group. Conclusions: Local delivery of KGF-1 might prevent RI-induced SG damage by reducing apoptosis. |
topic |
radioiodine salivary gland keratinocyte growth factor anti-apoptosis |
url |
https://www.mdpi.com/1660-4601/17/17/6322 |
work_keys_str_mv |
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