Culture condition, inoculum production and host response of a wild mushroom, Phlebopus portentosus strain CMUHH121-005

• Main Author: Saisamorn Lumyong
• Format: Article
• Language: English
• Published: Maejo University 2011-12-01
• Series: Maejo International Journal of Science and Technology
• Subjects: Phlebopus portentosus; mushroom cultivation; mycorrhizae; mushroom inoculum
• Online Access: http://www.mijst.mju.ac.th/vol5/413-425.pdf

The optimal media and growth conditions of an edible wild mushroom, Phlebopus portentosus strain CMUHH121-005, were investigated for inoculum production. The optimum temperature for mycelial growth was 30ºC and the optimum pH was 4.0. Malt extract and yeast extract as carbon and nitrogen sources respectively, with a C:N ratio of 10:1, were most suitable. The biomass production in 16 types of cereal grain media was investigated. The fungus was found to grow best in barley grain mixed with Murashige and Skoog solution over 30 days following inoculation. Further incubation at 30°C for 60 days in the dark caused numerous agglomerations of mycelia as if fruiting bodies were being formed. The data presented provide growth requirements that will be useful in a future development of P. portentosus as a cultivatable mycorrhizal mushroom.