Retrospective Screening and Analysis of mcr-1 and blaNDM in Gram-Negative Bacteria in China, 2010–2019
Currently, Gram-negative bacteria have developed multidrug and broad-spectrum drug resistance, and the numbers of species and strains carrying mcr or blaNDM genes are increasing. In this study, mcr-1 and blaNDM distribution of 12,858 Gram-negative bacteria isolated from wildlife, patients, livestock...
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Format: | Article |
Language: | English |
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Frontiers Media S.A.
2020-02-01
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Series: | Frontiers in Microbiology |
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Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.00121/full |
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doaj-f312b9f160f448c5a783a8f653897e8e |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rong Fan Chuchu Li Chuchu Li Ran Duan Shuai Qin Junrong Liang Meng Xiao Dongyue Lv Huaiqi Jing Xin Wang |
spellingShingle |
Rong Fan Chuchu Li Chuchu Li Ran Duan Shuai Qin Junrong Liang Meng Xiao Dongyue Lv Huaiqi Jing Xin Wang Retrospective Screening and Analysis of mcr-1 and blaNDM in Gram-Negative Bacteria in China, 2010–2019 Frontiers in Microbiology MCR NDM polymyxin carbapenem Gram-negative |
author_facet |
Rong Fan Chuchu Li Chuchu Li Ran Duan Shuai Qin Junrong Liang Meng Xiao Dongyue Lv Huaiqi Jing Xin Wang |
author_sort |
Rong Fan |
title |
Retrospective Screening and Analysis of mcr-1 and blaNDM in Gram-Negative Bacteria in China, 2010–2019 |
title_short |
Retrospective Screening and Analysis of mcr-1 and blaNDM in Gram-Negative Bacteria in China, 2010–2019 |
title_full |
Retrospective Screening and Analysis of mcr-1 and blaNDM in Gram-Negative Bacteria in China, 2010–2019 |
title_fullStr |
Retrospective Screening and Analysis of mcr-1 and blaNDM in Gram-Negative Bacteria in China, 2010–2019 |
title_full_unstemmed |
Retrospective Screening and Analysis of mcr-1 and blaNDM in Gram-Negative Bacteria in China, 2010–2019 |
title_sort |
retrospective screening and analysis of mcr-1 and blandm in gram-negative bacteria in china, 2010–2019 |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2020-02-01 |
description |
Currently, Gram-negative bacteria have developed multidrug and broad-spectrum drug resistance, and the numbers of species and strains carrying mcr or blaNDM genes are increasing. In this study, mcr-1 and blaNDM distribution of 12,858 Gram-negative bacteria isolated from wildlife, patients, livestock, poultry and environment in 14 provinces of China from 2010 to 2019 and the antibiotics resistance in regard to polymyxins (polymyxin B and colistin) and carbapenems of positive strains were investigated. A total of 70 strains of 10 species carried the mcr-1 gene, positive rates of patients, livestock and poultry, and environmental strains were 0.62% (36/5,828), 4.07% (29/712), 5.43% (5/92), respectively. Six strains of 3 species carrying the blaNDM gene all came from patients 0.10% (6/5,828). Two new mcr-1 gene variants (GenBank: MK965883, MK965884) were identified, one of which contains premature stop codon. The drug susceptibility results showed that all mcr-1 carriers were sensitive to carbapenems, among which, 66 strains were resistant and 4 were sensitive to polymyxins. The strains with the blaNDM gene had different degrees of resistance to carbapenems and were sensitive to polymyxins. The findings that species carrying mcr-1 or blaNDM genes were limited and mostly normal flora of opportunistic or low pathogenic organisms indicated that transfer of mcr-1 and blaNDM genes between bacteria was relatively limited in China. The none detection among wildlife compared with other sources supports the speculation that the emergence of and increase in polymyxins and carbapenem-resistant strains was mainly related to the selective pressure of antibiotics. |
topic |
MCR NDM polymyxin carbapenem Gram-negative |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2020.00121/full |
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AT rongfan retrospectivescreeningandanalysisofmcr1andblandmingramnegativebacteriainchina20102019 AT chuchuli retrospectivescreeningandanalysisofmcr1andblandmingramnegativebacteriainchina20102019 AT chuchuli retrospectivescreeningandanalysisofmcr1andblandmingramnegativebacteriainchina20102019 AT randuan retrospectivescreeningandanalysisofmcr1andblandmingramnegativebacteriainchina20102019 AT shuaiqin retrospectivescreeningandanalysisofmcr1andblandmingramnegativebacteriainchina20102019 AT junrongliang retrospectivescreeningandanalysisofmcr1andblandmingramnegativebacteriainchina20102019 AT mengxiao retrospectivescreeningandanalysisofmcr1andblandmingramnegativebacteriainchina20102019 AT dongyuelv retrospectivescreeningandanalysisofmcr1andblandmingramnegativebacteriainchina20102019 AT huaiqijing retrospectivescreeningandanalysisofmcr1andblandmingramnegativebacteriainchina20102019 AT xinwang retrospectivescreeningandanalysisofmcr1andblandmingramnegativebacteriainchina20102019 |
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spelling |
doaj-f312b9f160f448c5a783a8f653897e8e2020-11-25T02:11:04ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-02-011110.3389/fmicb.2020.00121495541Retrospective Screening and Analysis of mcr-1 and blaNDM in Gram-Negative Bacteria in China, 2010–2019Rong Fan0Chuchu Li1Chuchu Li2Ran Duan3Shuai Qin4Junrong Liang5Meng Xiao6Dongyue Lv7Huaiqi Jing8Xin Wang9State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases – National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaState Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases – National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaDepartment of Acute Infectious Disease Control and Prevention, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, ChinaState Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases – National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaState Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases – National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaState Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases – National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaState Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases – National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaState Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases – National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaState Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases – National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaState Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases – National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaCurrently, Gram-negative bacteria have developed multidrug and broad-spectrum drug resistance, and the numbers of species and strains carrying mcr or blaNDM genes are increasing. In this study, mcr-1 and blaNDM distribution of 12,858 Gram-negative bacteria isolated from wildlife, patients, livestock, poultry and environment in 14 provinces of China from 2010 to 2019 and the antibiotics resistance in regard to polymyxins (polymyxin B and colistin) and carbapenems of positive strains were investigated. A total of 70 strains of 10 species carried the mcr-1 gene, positive rates of patients, livestock and poultry, and environmental strains were 0.62% (36/5,828), 4.07% (29/712), 5.43% (5/92), respectively. Six strains of 3 species carrying the blaNDM gene all came from patients 0.10% (6/5,828). Two new mcr-1 gene variants (GenBank: MK965883, MK965884) were identified, one of which contains premature stop codon. The drug susceptibility results showed that all mcr-1 carriers were sensitive to carbapenems, among which, 66 strains were resistant and 4 were sensitive to polymyxins. The strains with the blaNDM gene had different degrees of resistance to carbapenems and were sensitive to polymyxins. The findings that species carrying mcr-1 or blaNDM genes were limited and mostly normal flora of opportunistic or low pathogenic organisms indicated that transfer of mcr-1 and blaNDM genes between bacteria was relatively limited in China. The none detection among wildlife compared with other sources supports the speculation that the emergence of and increase in polymyxins and carbapenem-resistant strains was mainly related to the selective pressure of antibiotics.https://www.frontiersin.org/article/10.3389/fmicb.2020.00121/fullMCRNDMpolymyxincarbapenemGram-negative |