Influence of a Pulsed Electric Field on Charge Generation in a Flowing Protein Solution

• Main Authors: Yuri D. Ivanov, Andrey F. Kozlov, Rafael A. Galiullin, Vadim Yu. Tatur, Vadim S. Ziborov, Sergey A. Usanov, Tatyana O. Pleshakova
• Format: Article
• Language: English
• Published: MDPI AG 2018-05-01
• Series: Separations
• Subjects: analytical flow-based systems; flowing protein solution; charge accumulation
• Online Access: http://www.mdpi.com/2297-8739/5/2/29

It is known that a charge is generated in water and protein solutions upon their motion; this phenomenon is also observed in analytical systems for atomic force microscopy (AFM)-based fishing. At that, the efficiency of protein fishing correlates with the value of charge, generated upon the motion of the analyzed solution. Earlier, we demonstrated that a pulsed electric field can well be used for the enhancement of the efficiency of AFM-based fishing of low-abundant protein. In this paper, we have demonstrated the influence of a pulsed electric field on the stimulation of the electric charge generation in a solution of low-abundant proteins observed in the injector part of an AFM-based fishing system at 23 °C and 38 °C. Taking this effect into account is important for the development of novel highly sensitive flow-based diagnostic systems, as well as for the development of models describing the influence of a pulsed electric field on pathological processes in the body, hemodynamics, and physicochemical properties of solutions.