The Effect of Pore Size Distribution and L-Lysine Modified Apatite Whiskers (HAP) on Osteoblasts Response in PLLA/HAP foam Scaffolds Obtained in the Thermally Induced Phase Separation Process

• Main Authors: Konrad Szustakiewicz, Marcin Włodarczyk, Małgorzata Gazińska, Karolina Rudnicka, Przemysław Płociński, Patrycja Szymczyk-Ziółkowska, Grzegorz Ziółkowski, Monika Biernat, Katarzyna Sieja, Michał Grzymajło, Piotr Jóźwiak, Sylwia Michlewska, Andrzej W. Trochimczuk
• Format: Article
• Language: English
• Published: MDPI AG 2021-03-01
• Series: International Journal of Molecular Sciences
• Subjects: polymers; foam scaffolds; PLLA; hydroxyapatite; cell adhesion; TIPS
• Online Access: https://www.mdpi.com/1422-0067/22/7/3607

In this research, we prepared foam scaffolds based on poly(L-lactide) (PLLA) and apatite whiskers (HAP) using thermally induced phase separation technique supported by the salt leaching process (TIPS-SL). Using sodium chloride having a size of a) 150–315 μm, b) 315–400 μm, and c) 500–600 μm, three types of foams with different pore sizes have been obtained. Internal structure of the obtained materials has been investigated using SEM as well as μCT. The materials have been studied by means of porosity, density, and compression tests. As the most promising, the composite prepared with salt size of 500–600 μm was prepared also with the L-lysine modified apatite. The osteoblast hFOB 1.19 cell response for the scaffolds was also investigated by means of cell viability, proliferation, adhesion/penetration, and biomineralization. Direct contact cytotoxicity assay showed the cytocompatibility of the scaffolds. All types of foam scaffolds containing HAP whiskers, regardless the pore size or L-lysine modification induced significant stimulatory effect on the cal-cium deposits formation in osteoblasts. The PLLA/HAP scaffolds modified with L-lysine stimulated hFOB 1.19 osteoblasts proliferation. Compared to the scaffolds with smaller pores (150–315 µm and 315–400 µm), the PLLA/HAP foams with large pores (500–600 µm) promoted more effective ad-hesion of osteoblasts to the surface of the biomaterial.