Prevalence of Antibiotic Resistome in Ready-to-Eat Salad
Ready-to-eat salad harbors microorganisms that may carry various antibiotic resistance genes (ARGs). However, few studies have focused on the prevalence of ARGs on salad, thus underestimating the risk of ARGs transferring from salad to consumers. In this small-scale study, high-throughput quantitati...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2020-03-01
|
Series: | Frontiers in Public Health |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fpubh.2020.00092/full |
id |
doaj-f331b27098e84bcaafbd5d182fbb59ff |
---|---|
record_format |
Article |
spelling |
doaj-f331b27098e84bcaafbd5d182fbb59ff2020-11-25T02:05:08ZengFrontiers Media S.A.Frontiers in Public Health2296-25652020-03-01810.3389/fpubh.2020.00092513102Prevalence of Antibiotic Resistome in Ready-to-Eat SaladShu-Yi-Dan Zhou0Shu-Yi-Dan Zhou1Meng-Yun Wei2Meng-Yun Wei3Madeline Giles4Roy Neilson5Fei Zheng6Fei Zheng7Qi Zhang8Yong-Guan Zhu9Yong-Guan Zhu10Yong-Guan Zhu11Xiao-Ru Yang12Xiao-Ru Yang13Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, ChinaUniversity of the Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, ChinaUniversity of the Chinese Academy of Sciences, Beijing, ChinaEcological Sciences, The James Hutton Institute, Dundee, United KingdomEcological Sciences, The James Hutton Institute, Dundee, United KingdomKey Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, ChinaUniversity of the Chinese Academy of Sciences, Beijing, ChinaCollege of Environment, Zhejiang University of Technology, Hangzhou, ChinaKey Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, ChinaUniversity of the Chinese Academy of Sciences, Beijing, ChinaCenter for Excellence in Regional Atmospheric Environment, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, ChinaKey Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, ChinaCenter for Excellence in Regional Atmospheric Environment, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, ChinaReady-to-eat salad harbors microorganisms that may carry various antibiotic resistance genes (ARGs). However, few studies have focused on the prevalence of ARGs on salad, thus underestimating the risk of ARGs transferring from salad to consumers. In this small-scale study, high-throughput quantitative PCR was used to explore the presence, prevalence and abundance of ARGs associated with serving salad sourced from two restaurant types, fast-food chain and independent casual dining. A total of 156 unique ARGs and nine mobile genetic elements (MGEs) were detected on the salad items assessed. The abundance of ARGs and MGEs were significantly higher in independent casual dining than fast-food chain restaurants. Absolute copies of ARGs in salad were 1.34 × 107 to 2.71 × 108 and 1.90 × 108 to 4.87 × 108 copies per g salad in fast-food and casual dining restaurants, respectively. Proteobacteria, Bacteroidetes, Actinobacteria, and Firmicutes were the dominant bacterial phyla detected from salad samples. Pseudomonas, Acinetobacter, Exiguobacterium, Weissella, Enterobacter, Leuconostoc, Pantoea, Serratia, Erwinia, and Ewingella were the 10 most dominant bacterial genera found in salad samples. A significant positive correlation between ARGs and MGEs was detected. These results integrate knowledge about the ARGs in ready-to-eat salad and highlight the potential impact of ARGs transfer to consumers.https://www.frontiersin.org/article/10.3389/fpubh.2020.00092/fullready-to-eat foodsaladantibiotic resistance genes (ARGs)high-throughput quantitative PCRhuman health |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shu-Yi-Dan Zhou Shu-Yi-Dan Zhou Meng-Yun Wei Meng-Yun Wei Madeline Giles Roy Neilson Fei Zheng Fei Zheng Qi Zhang Yong-Guan Zhu Yong-Guan Zhu Yong-Guan Zhu Xiao-Ru Yang Xiao-Ru Yang |
spellingShingle |
Shu-Yi-Dan Zhou Shu-Yi-Dan Zhou Meng-Yun Wei Meng-Yun Wei Madeline Giles Roy Neilson Fei Zheng Fei Zheng Qi Zhang Yong-Guan Zhu Yong-Guan Zhu Yong-Guan Zhu Xiao-Ru Yang Xiao-Ru Yang Prevalence of Antibiotic Resistome in Ready-to-Eat Salad Frontiers in Public Health ready-to-eat food salad antibiotic resistance genes (ARGs) high-throughput quantitative PCR human health |
author_facet |
Shu-Yi-Dan Zhou Shu-Yi-Dan Zhou Meng-Yun Wei Meng-Yun Wei Madeline Giles Roy Neilson Fei Zheng Fei Zheng Qi Zhang Yong-Guan Zhu Yong-Guan Zhu Yong-Guan Zhu Xiao-Ru Yang Xiao-Ru Yang |
author_sort |
Shu-Yi-Dan Zhou |
title |
Prevalence of Antibiotic Resistome in Ready-to-Eat Salad |
title_short |
Prevalence of Antibiotic Resistome in Ready-to-Eat Salad |
title_full |
Prevalence of Antibiotic Resistome in Ready-to-Eat Salad |
title_fullStr |
Prevalence of Antibiotic Resistome in Ready-to-Eat Salad |
title_full_unstemmed |
Prevalence of Antibiotic Resistome in Ready-to-Eat Salad |
title_sort |
prevalence of antibiotic resistome in ready-to-eat salad |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Public Health |
issn |
2296-2565 |
publishDate |
2020-03-01 |
description |
Ready-to-eat salad harbors microorganisms that may carry various antibiotic resistance genes (ARGs). However, few studies have focused on the prevalence of ARGs on salad, thus underestimating the risk of ARGs transferring from salad to consumers. In this small-scale study, high-throughput quantitative PCR was used to explore the presence, prevalence and abundance of ARGs associated with serving salad sourced from two restaurant types, fast-food chain and independent casual dining. A total of 156 unique ARGs and nine mobile genetic elements (MGEs) were detected on the salad items assessed. The abundance of ARGs and MGEs were significantly higher in independent casual dining than fast-food chain restaurants. Absolute copies of ARGs in salad were 1.34 × 107 to 2.71 × 108 and 1.90 × 108 to 4.87 × 108 copies per g salad in fast-food and casual dining restaurants, respectively. Proteobacteria, Bacteroidetes, Actinobacteria, and Firmicutes were the dominant bacterial phyla detected from salad samples. Pseudomonas, Acinetobacter, Exiguobacterium, Weissella, Enterobacter, Leuconostoc, Pantoea, Serratia, Erwinia, and Ewingella were the 10 most dominant bacterial genera found in salad samples. A significant positive correlation between ARGs and MGEs was detected. These results integrate knowledge about the ARGs in ready-to-eat salad and highlight the potential impact of ARGs transfer to consumers. |
topic |
ready-to-eat food salad antibiotic resistance genes (ARGs) high-throughput quantitative PCR human health |
url |
https://www.frontiersin.org/article/10.3389/fpubh.2020.00092/full |
work_keys_str_mv |
AT shuyidanzhou prevalenceofantibioticresistomeinreadytoeatsalad AT shuyidanzhou prevalenceofantibioticresistomeinreadytoeatsalad AT mengyunwei prevalenceofantibioticresistomeinreadytoeatsalad AT mengyunwei prevalenceofantibioticresistomeinreadytoeatsalad AT madelinegiles prevalenceofantibioticresistomeinreadytoeatsalad AT royneilson prevalenceofantibioticresistomeinreadytoeatsalad AT feizheng prevalenceofantibioticresistomeinreadytoeatsalad AT feizheng prevalenceofantibioticresistomeinreadytoeatsalad AT qizhang prevalenceofantibioticresistomeinreadytoeatsalad AT yongguanzhu prevalenceofantibioticresistomeinreadytoeatsalad AT yongguanzhu prevalenceofantibioticresistomeinreadytoeatsalad AT yongguanzhu prevalenceofantibioticresistomeinreadytoeatsalad AT xiaoruyang prevalenceofantibioticresistomeinreadytoeatsalad AT xiaoruyang prevalenceofantibioticresistomeinreadytoeatsalad |
_version_ |
1724939765017477120 |