Polymorphisms in , a Transcriptional Target of TNF-α, Are Associated With Inflammatory Bowel Disease in Korean
Background/AimsEmerging data indicate that polymorphic sequence variations in the tumor necrosis factor alpha (TNF-α) gene may affect its production, and be associated with the risk of inflammatory bowel disease (IBD). PRKCDBP is a putative tumor suppressor gene and a transcriptional target of TNF-α...
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Korean Association for the Study of Intestinal Diseases
2015-07-01
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doaj-f33973a29e03430889ae8c1cf07de7c72020-11-25T01:12:19ZengKorean Association for the Study of Intestinal DiseasesIntestinal Research1598-91002288-19562015-07-0113324224910.5217/ir.2015.13.3.24290Polymorphisms in , a Transcriptional Target of TNF-α, Are Associated With Inflammatory Bowel Disease in KoreanJung-Wook Kim0Chang Kyun Lee1Hyo Jong Kim2Jae-Jun Shim3Jae Young Jang4Seok Ho Dong5Byung-Ho Kim6Young Woon Chang7Sung-Gil Chi8Division of Gastroenterology, Department of Internal Medicine, Kyung Hee University School of Medicine, Seoul, Korea.Division of Gastroenterology, Department of Internal Medicine, Kyung Hee University School of Medicine, Seoul, Korea.Division of Gastroenterology, Department of Internal Medicine, Kyung Hee University School of Medicine, Seoul, Korea.Division of Gastroenterology, Department of Internal Medicine, Kyung Hee University School of Medicine, Seoul, Korea.Division of Gastroenterology, Department of Internal Medicine, Kyung Hee University School of Medicine, Seoul, Korea.Division of Gastroenterology, Department of Internal Medicine, Kyung Hee University School of Medicine, Seoul, Korea.Division of Gastroenterology, Department of Internal Medicine, Kyung Hee University School of Medicine, Seoul, Korea.Division of Gastroenterology, Department of Internal Medicine, Kyung Hee University School of Medicine, Seoul, Korea.School of Life Sciences and Biotechnology, Korea University, Seoul, Korea.Background/AimsEmerging data indicate that polymorphic sequence variations in the tumor necrosis factor alpha (TNF-α) gene may affect its production, and be associated with the risk of inflammatory bowel disease (IBD). PRKCDBP is a putative tumor suppressor gene and a transcriptional target of TNF-α. The aim of this case-control study is to explore the possible association of single nucleotide polymorphisms (SNPs) in PRKCDBP with the development of IBD in Koreans.MethodsGenotyping analysis of four SNPs of PRKCDBP [rs35301211 (G210A), rs11544766 (G237C), rs12294600 (C797T), and rs1051992 (T507C)] was performed on 170 ulcerative colitis (UC),131 Crohn's disease (CD) patients, and 100 unrelated healthy controls using polymerase chain reaction and restriction fragment length polymorphism.ResultsHeterozygous configuration of three SNPs (G210A, G237C, and C797T) was very rare in both patients and healthy controls. However, allele frequencies of the T507C SNP showed a significant difference between UC patients and controls (P=0.037). The CC genotype of the T507C SNP was identified in 46.6% (61 of 131) of CD and 49.4% (84 of 170) of UC patients, but only in 33.0% (33 of 100) of healthy controls. Furthermore, CC homozygosity was more prevalent than TC heterozygosity in both CD and UC patients versus controls (P=0.016; gender-adjusted odds ratio [aOR], 2.16; 95% confidence interval [CI], 1.16-4.04 and P=0.009; aOR, 2.09; 95% CI, 1.193.64; respectively)ConclusionsOur results suggest that the T507C SNP in PRKCDBP, a TNF-α-inducible gene, might be associated with susceptibility to IBD (particularly UC) development in Koreans.http://www.irjournal.org/upload/pdf/ir-13-242.pdfPolymorphisms, single nucleotideCrohn diseaseColitis, ulcerative |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jung-Wook Kim Chang Kyun Lee Hyo Jong Kim Jae-Jun Shim Jae Young Jang Seok Ho Dong Byung-Ho Kim Young Woon Chang Sung-Gil Chi |
spellingShingle |
Jung-Wook Kim Chang Kyun Lee Hyo Jong Kim Jae-Jun Shim Jae Young Jang Seok Ho Dong Byung-Ho Kim Young Woon Chang Sung-Gil Chi Polymorphisms in , a Transcriptional Target of TNF-α, Are Associated With Inflammatory Bowel Disease in Korean Intestinal Research Polymorphisms, single nucleotide Crohn disease Colitis, ulcerative |
author_facet |
Jung-Wook Kim Chang Kyun Lee Hyo Jong Kim Jae-Jun Shim Jae Young Jang Seok Ho Dong Byung-Ho Kim Young Woon Chang Sung-Gil Chi |
author_sort |
Jung-Wook Kim |
title |
Polymorphisms in , a Transcriptional Target of TNF-α, Are Associated With Inflammatory Bowel Disease in Korean |
title_short |
Polymorphisms in , a Transcriptional Target of TNF-α, Are Associated With Inflammatory Bowel Disease in Korean |
title_full |
Polymorphisms in , a Transcriptional Target of TNF-α, Are Associated With Inflammatory Bowel Disease in Korean |
title_fullStr |
Polymorphisms in , a Transcriptional Target of TNF-α, Are Associated With Inflammatory Bowel Disease in Korean |
title_full_unstemmed |
Polymorphisms in , a Transcriptional Target of TNF-α, Are Associated With Inflammatory Bowel Disease in Korean |
title_sort |
polymorphisms in , a transcriptional target of tnf-α, are associated with inflammatory bowel disease in korean |
publisher |
Korean Association for the Study of Intestinal Diseases |
series |
Intestinal Research |
issn |
1598-9100 2288-1956 |
publishDate |
2015-07-01 |
description |
Background/AimsEmerging data indicate that polymorphic sequence variations in the tumor necrosis factor alpha (TNF-α) gene may affect its production, and be associated with the risk of inflammatory bowel disease (IBD). PRKCDBP is a putative tumor suppressor gene and a transcriptional target of TNF-α. The aim of this case-control study is to explore the possible association of single nucleotide polymorphisms (SNPs) in PRKCDBP with the development of IBD in Koreans.MethodsGenotyping analysis of four SNPs of PRKCDBP [rs35301211 (G210A), rs11544766 (G237C), rs12294600 (C797T), and rs1051992 (T507C)] was performed on 170 ulcerative colitis (UC),131 Crohn's disease (CD) patients, and 100 unrelated healthy controls using polymerase chain reaction and restriction fragment length polymorphism.ResultsHeterozygous configuration of three SNPs (G210A, G237C, and C797T) was very rare in both patients and healthy controls. However, allele frequencies of the T507C SNP showed a significant difference between UC patients and controls (P=0.037). The CC genotype of the T507C SNP was identified in 46.6% (61 of 131) of CD and 49.4% (84 of 170) of UC patients, but only in 33.0% (33 of 100) of healthy controls. Furthermore, CC homozygosity was more prevalent than TC heterozygosity in both CD and UC patients versus controls (P=0.016; gender-adjusted odds ratio [aOR], 2.16; 95% confidence interval [CI], 1.16-4.04 and P=0.009; aOR, 2.09; 95% CI, 1.193.64; respectively)ConclusionsOur results suggest that the T507C SNP in PRKCDBP, a TNF-α-inducible gene, might be associated with susceptibility to IBD (particularly UC) development in Koreans. |
topic |
Polymorphisms, single nucleotide Crohn disease Colitis, ulcerative |
url |
http://www.irjournal.org/upload/pdf/ir-13-242.pdf |
work_keys_str_mv |
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