Epicatechins Purified from Green Tea (Camellia sinensis) Differentially Suppress Growth of Gender-Dependent Human Cancer Cell Lines
The anticancer potential of catechins derived from green tea is not well understood, in part because catechin-related growth suppression and/or apoptosis appears to vary with the type and stage of malignancy as well as with the type of catechin. This in vitro study examined the biological effects o...
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Online Access: | http://dx.doi.org/10.1093/ecam/nel003 |
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doaj-f352b41449ad4b1eb17a6eacaf75aaec2020-11-24T23:54:06ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882006-01-013223724710.1093/ecam/nel003Epicatechins Purified from Green Tea (Camellia sinensis) Differentially Suppress Growth of Gender-Dependent Human Cancer Cell LinesMepur H. Ravindranath0Thiruverkadu S. Saravanan1Clarence C. Monteclaro2Naftali Presser3Xing Ye4Senthamil R. Selvan5Stanley Brosman6Department of Glycoimmunotherapy, John Wayne Cancer Institute Santa Monica, CA, USADepartment of Glycoimmunotherapy, John Wayne Cancer Institute Santa Monica, CA, USADepartment of Glycoimmunotherapy, John Wayne Cancer Institute Santa Monica, CA, USADepartment of Glycoimmunotherapy, John Wayne Cancer Institute Santa Monica, CA, USADepartment of Glycoimmunotherapy, John Wayne Cancer Institute Santa Monica, CA, USACell Biology Laboratory, Hoag Cancer Center, Hoag Memorial Hospital Presbyterian Newport Beach, CA, USAPacific Clinical Research Santa Monica, CA, USAThe anticancer potential of catechins derived from green tea is not well understood, in part because catechin-related growth suppression and/or apoptosis appears to vary with the type and stage of malignancy as well as with the type of catechin. This in vitro study examined the biological effects of epicatechin (EC), epigallocatechin (EGC), EC 3-gallate (ECG) and EGC 3-gallate (EGCG) in cell lines from human gender-specific cancers. Cell lines developed from organ-confined (HH870) and metastatic (DU145) prostate cancer, and from moderately (HH450) and poorly differentiated (HH639) epithelial ovarian cancer were grown with or without EC, EGC, ECG or EGCG. When untreated cells reached confluency, viability and doubling time were measured for treated and untreated cells. Whereas EC treatment reduced proliferation of HH639 cells by 50%, EGCG suppressed proliferation of all cell lines by 50%. ECG was even more potent: it inhibited DU145, HH870, HH450 and HH639 cells at concentrations of 24, 27, 29 and 30 µM, whereas EGCG inhibited DU145, HH870, HH450 and HH639 cells at concentrations 89, 45, 62 and 42 µM. When compared with EGCG, ECG more effectively suppresses the growth of prostate cancer and epithelial ovarian cancer cell lines derived from tumors of patients with different stages of disease.http://dx.doi.org/10.1093/ecam/nel003 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mepur H. Ravindranath Thiruverkadu S. Saravanan Clarence C. Monteclaro Naftali Presser Xing Ye Senthamil R. Selvan Stanley Brosman |
spellingShingle |
Mepur H. Ravindranath Thiruverkadu S. Saravanan Clarence C. Monteclaro Naftali Presser Xing Ye Senthamil R. Selvan Stanley Brosman Epicatechins Purified from Green Tea (Camellia sinensis) Differentially Suppress Growth of Gender-Dependent Human Cancer Cell Lines Evidence-Based Complementary and Alternative Medicine |
author_facet |
Mepur H. Ravindranath Thiruverkadu S. Saravanan Clarence C. Monteclaro Naftali Presser Xing Ye Senthamil R. Selvan Stanley Brosman |
author_sort |
Mepur H. Ravindranath |
title |
Epicatechins Purified from Green Tea (Camellia sinensis) Differentially Suppress Growth of Gender-Dependent Human Cancer Cell Lines |
title_short |
Epicatechins Purified from Green Tea (Camellia sinensis) Differentially Suppress Growth of Gender-Dependent Human Cancer Cell Lines |
title_full |
Epicatechins Purified from Green Tea (Camellia sinensis) Differentially Suppress Growth of Gender-Dependent Human Cancer Cell Lines |
title_fullStr |
Epicatechins Purified from Green Tea (Camellia sinensis) Differentially Suppress Growth of Gender-Dependent Human Cancer Cell Lines |
title_full_unstemmed |
Epicatechins Purified from Green Tea (Camellia sinensis) Differentially Suppress Growth of Gender-Dependent Human Cancer Cell Lines |
title_sort |
epicatechins purified from green tea (camellia sinensis) differentially suppress growth of gender-dependent human cancer cell lines |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2006-01-01 |
description |
The anticancer potential of catechins derived from green tea is not well understood, in part because catechin-related growth suppression and/or apoptosis appears to vary with the type and stage of malignancy as well as with the type of catechin. This in vitro study examined the biological effects of epicatechin (EC), epigallocatechin (EGC), EC 3-gallate (ECG) and EGC 3-gallate (EGCG) in cell lines from human gender-specific cancers. Cell lines developed from organ-confined (HH870) and metastatic (DU145) prostate cancer, and from moderately (HH450) and poorly differentiated (HH639) epithelial ovarian cancer were grown with or without EC, EGC, ECG or EGCG. When untreated cells reached confluency, viability and doubling time were measured for treated and untreated cells. Whereas EC treatment reduced proliferation of HH639 cells by 50%, EGCG suppressed proliferation of all cell lines by 50%. ECG was even more potent: it inhibited DU145, HH870, HH450 and HH639 cells at concentrations of 24, 27, 29 and 30 µM, whereas EGCG inhibited DU145, HH870, HH450 and HH639 cells at concentrations 89, 45, 62 and 42 µM. When compared with EGCG, ECG more effectively suppresses the growth of prostate cancer and epithelial ovarian cancer cell lines derived from tumors of patients with different stages of disease. |
url |
http://dx.doi.org/10.1093/ecam/nel003 |
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