Glycine Improves Ischemic Stroke Through miR-19a-3p/AMPK/GSK-3β/HO-1 Pathway
Zhong-Jun Chen, Xu-Sheng Zhao, Tie-Ping Fan, Heng-Xu Qi, Di Li Neurological Intervention Department, Dalian Municipal Central Hospital, Dalian, Liaoning Province, People’s Republic of ChinaCorrespondence: Di LiNeurological Intervention Department, Dalian Municipal Central Hospital, Dalian,...
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doaj-f358b58634a94460b93c8022205a57de2020-11-25T03:00:02ZengDove Medical PressDrug Design, Development and Therapy1177-88812020-05-01Volume 142021203154053Glycine Improves Ischemic Stroke Through miR-19a-3p/AMPK/GSK-3β/HO-1 PathwayChen ZJZhao XSFan TPQi HXLi DZhong-Jun Chen, Xu-Sheng Zhao, Tie-Ping Fan, Heng-Xu Qi, Di Li Neurological Intervention Department, Dalian Municipal Central Hospital, Dalian, Liaoning Province, People’s Republic of ChinaCorrespondence: Di LiNeurological Intervention Department, Dalian Municipal Central Hospital, Dalian, Liaoning Province, People’s Republic of ChinaTel +86 411-84412001-8481Email xiangqing14740@163.comPurpose: To explore the molecular mechanism of glycine in improving ischemic stroke.Patients and Methods: The serum samples of patients with ischemic stroke and healthy people were compared. The ischemic stroke model of PC12 cells was established by oxygen-glucose deprivation (OGD). qPCR quantified miR-19a-3p and AMPK mRNA, and protein expression was detected by Western blot. MTT was used to detect cell activity. Flow cytometry was used to detect cells. Glucose metabolism kit was used to detect glucose intake and formation amount of lactic acid.Results: Compared with the control group, OGD group (OGDG) showed lower cell activity and increased cell apoptosis. TNF-α, IL-1βI, L-6, Caspase 3, Caspase 9 and Bax were up-regulated, and Glut1, HK2, LDHA, PDK1, PKM2 and Bcl2 were down-regulated. At the same time, glucose intake, formation amount of lactic acid and cell apoptosis rate were reduced, and AMPK/GSK-3β/HO-1 pathway activity was down-regulated. Glycine could counteract the above phenomena in OGDG. miR-19a-3p and AMPK decreased and increased, respectively, during glycine therapy. AMPK was the target gene of miR-19a-3p. Rescue experiments demonstrated that glycine improved cell apoptosis, inflammatory response and glucose metabolism disorder of ischemic stroke through miR-19a-3p/AMPK/GSK-3β/HO-1 pathway.Conclusion: Glycine improves ischemic stroke through miR-19a-3p/AMPK/GSK-3β/HO-1 pathway.Keywords: ischemic stroke, AMPK/GSK-3β/HO-1 pathway, miR-19a-3p, glycinehttps://www.dovepress.com/glycine-improves-ischemic-stroke-through-mir-19a-3pampkgsk-3betaho-1-p-peer-reviewed-article-DDDTischemic strokeampk/gsk-3β/ho-1 pathwaymir-19a-3pglycine |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chen ZJ Zhao XS Fan TP Qi HX Li D |
spellingShingle |
Chen ZJ Zhao XS Fan TP Qi HX Li D Glycine Improves Ischemic Stroke Through miR-19a-3p/AMPK/GSK-3β/HO-1 Pathway Drug Design, Development and Therapy ischemic stroke ampk/gsk-3β/ho-1 pathway mir-19a-3p glycine |
author_facet |
Chen ZJ Zhao XS Fan TP Qi HX Li D |
author_sort |
Chen ZJ |
title |
Glycine Improves Ischemic Stroke Through miR-19a-3p/AMPK/GSK-3β/HO-1 Pathway |
title_short |
Glycine Improves Ischemic Stroke Through miR-19a-3p/AMPK/GSK-3β/HO-1 Pathway |
title_full |
Glycine Improves Ischemic Stroke Through miR-19a-3p/AMPK/GSK-3β/HO-1 Pathway |
title_fullStr |
Glycine Improves Ischemic Stroke Through miR-19a-3p/AMPK/GSK-3β/HO-1 Pathway |
title_full_unstemmed |
Glycine Improves Ischemic Stroke Through miR-19a-3p/AMPK/GSK-3β/HO-1 Pathway |
title_sort |
glycine improves ischemic stroke through mir-19a-3p/ampk/gsk-3β/ho-1 pathway |
publisher |
Dove Medical Press |
series |
Drug Design, Development and Therapy |
issn |
1177-8881 |
publishDate |
2020-05-01 |
description |
Zhong-Jun Chen, Xu-Sheng Zhao, Tie-Ping Fan, Heng-Xu Qi, Di Li Neurological Intervention Department, Dalian Municipal Central Hospital, Dalian, Liaoning Province, People’s Republic of ChinaCorrespondence: Di LiNeurological Intervention Department, Dalian Municipal Central Hospital, Dalian, Liaoning Province, People’s Republic of ChinaTel +86 411-84412001-8481Email xiangqing14740@163.comPurpose: To explore the molecular mechanism of glycine in improving ischemic stroke.Patients and Methods: The serum samples of patients with ischemic stroke and healthy people were compared. The ischemic stroke model of PC12 cells was established by oxygen-glucose deprivation (OGD). qPCR quantified miR-19a-3p and AMPK mRNA, and protein expression was detected by Western blot. MTT was used to detect cell activity. Flow cytometry was used to detect cells. Glucose metabolism kit was used to detect glucose intake and formation amount of lactic acid.Results: Compared with the control group, OGD group (OGDG) showed lower cell activity and increased cell apoptosis. TNF-α, IL-1βI, L-6, Caspase 3, Caspase 9 and Bax were up-regulated, and Glut1, HK2, LDHA, PDK1, PKM2 and Bcl2 were down-regulated. At the same time, glucose intake, formation amount of lactic acid and cell apoptosis rate were reduced, and AMPK/GSK-3β/HO-1 pathway activity was down-regulated. Glycine could counteract the above phenomena in OGDG. miR-19a-3p and AMPK decreased and increased, respectively, during glycine therapy. AMPK was the target gene of miR-19a-3p. Rescue experiments demonstrated that glycine improved cell apoptosis, inflammatory response and glucose metabolism disorder of ischemic stroke through miR-19a-3p/AMPK/GSK-3β/HO-1 pathway.Conclusion: Glycine improves ischemic stroke through miR-19a-3p/AMPK/GSK-3β/HO-1 pathway.Keywords: ischemic stroke, AMPK/GSK-3β/HO-1 pathway, miR-19a-3p, glycine |
topic |
ischemic stroke ampk/gsk-3β/ho-1 pathway mir-19a-3p glycine |
url |
https://www.dovepress.com/glycine-improves-ischemic-stroke-through-mir-19a-3pampkgsk-3betaho-1-p-peer-reviewed-article-DDDT |
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