HIV-1 Integrase-DNA Recognition Mechanisms

HIV-1 Integrase-DNA Recognition Mechanisms

Integration of a reverse transcribed DNA copy of the HIV viral genome into the host chromosome is essential for virus replication. This process is catalyzed by the virally encoded protein integrase. The catalytic activities, which involve DNA cutting and joining steps, have been recapitulated in vit...

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Bibliographic Details
Main Authors: Mamuka Kvaratskhelia, Ari Katz, Nikolozi Shkriabai, Jocelyn O. Eidahl, Christopher J. McKee, Jacques J. Kessl
Format: Article
Language:English
Published: MDPI AG 2009-11-01
Series:Viruses
Subjects:
HIV
retroviruses
DNA
integrase
crosslinking
footprinting
Online Access:http://www.mdpi.com/1999-4915/1/3/713/
Description
Summary:Integration of a reverse transcribed DNA copy of the HIV viral genome into the host chromosome is essential for virus replication. This process is catalyzed by the virally encoded protein integrase. The catalytic activities, which involve DNA cutting and joining steps, have been recapitulated in vitro using recombinant integrase and synthetic DNA substrates. Biochemical and biophysical studies of these model reactions have been pivotal in advancing our understanding of mechanistic details for how IN interacts with viral and target DNAs, and are the focus of the present review.
ISSN:1999-4915

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